PubMed Health⌕ Search

Biomedical subjects

B Vandvik

Publications and source records attributed to B Vandvik.

At least 55 records · Page 3Linked to original sources
PubMed1978

Mumps meningitis: prolonged pleocytosis and occurrence of mumps virus-specific oligoclonal IgG in the cerebrospinal fluid.

A pleocytosis of the CSF occurred in all of 10 children with mumps meningitis and persisted for weeks and months in some patients. Oligoclonal IgG proteins were detected in the cerebrospinal fluid (CSF) during the 2nd week after onset of meningitis or later in 4 out of 10 patients, and could be detected as late as 11 and 12 months after meningitis in 2 patients. Evidence is presented that the oligoclonal IgG represents mumps virus-specific antibody synthesized locally in the brain. Oligoclonal virus antibodies were demonstrated also in serum samples. The persistence for weeks and moths of pleocytosis and oligoclonal IgG virus antibody in the CSF may imply that a virus infection in some cases persists in the brain in spite of apparently complete clinical recovery.

Adolescent↗
PubMed1978

Local synthesis in the central nervous system of diclonal IgM-kappa and homogeneous free kappa light chain proteins in a case of chronic meningoencephalitis.

A 60-year-old man with chronic meningoencephalitis and monuclear pleocytosis of the CSF was observed for 22 months. About one half of the CSF cells were concluded to represent IgM-synthesizing lymphocytes and plasma cells. Electrophoretic analysis disclosed two abnormal protein bands in the CSF. The bands were shown to be composed of a diclonal IgMk protein and a homogeneous k free light chain protein. No abnormal immunoglobulins were detected in serum or urine. The pleocytosis and immunoglobulin abnormalities of the CSF persisted during the observation period. Specific antibody activity of the diclonal IgM of the CSF was not demonstrated and the cause of the illness remains unknown.

Central Nervous System↗
PubMed1977

HLA antigens in multiple sclerosis.

The frequency of the MLC activating HLA-Dw2 determinant was found significantly increased to 68% among 37 patients with long-standing multiple sclerosis, compared to 30.2% among healthy controls. A similar (or stronger) degree of association was found with an "Ia-like" B cell specificity, SOW, while the association with HLA-B7 was less apparent. No correlation between HLA-Dw2 and signs of local synthesis of oligoclonal IgG or measles or rubella virus antibodies in the CNS was observed.

B-Lymphocytes↗
PubMed1977

Idiotypy of measles virus nucleocapsid-specific IgGkappa antibody in serum and cerebrospinal fluid in subacute sclerosing panencephalitis.

A gel-precipitating anti-idiotypic antiserum was prepared in a rabbit by immunization with measles virus-specific IgG isolated from the serum of a patient with subacute sclerosing pancencephalitis (SSPE). The idiotypic determinant was associated with an electrophoretically restricted and cathodally migrating IgGkappa protein in serum and cerebrospinal fluid (CSF) with antibody activity to measles nucleocapsids. The anti-idiotypic antiserum did not precipitate with other oligoclonal populations of measles-specific IgG in the patients serum or CSF, nor with serum or CSF from 4 other patients with SSPE and 10 patients with multiple sclerosis.

Antibodies, Viral↗
PubMed1977

Evidence of local synthesis of smooth-muscle antibodies in the central nervous system in isolated cases of multiple sclerosis and chronic lymphocytic meningoencephalitis.

Paired samples of serum and concentrated cerebrospinal fluid (CSF) from 53 patients with multiple sclerosis (MS) and from 36 patients with other neurological diseases were adjusted to an equal concentration of IgG and examined for occurrence of auto-antibodies. Anti-nuclear antibodies, anti-mitochondrial antibodies, and rheumatoid factor were not detected with increased frequency in the materials studied. Smooth-muscle antibody (SMA) was, however, detected in CSF and serum from three patients with MS and one patient with chronic lymphocytic meningoencephalitis, and in the CSF only from one additional patient with MS. The SMA activities of the CSF were 4 to 16 times higher than in the matching sera, suggesting a local SMA synthesis within the central nervous system. The SMA was associated with electrophoretically restricted fractions of IgG, but an association between SMA and oligoclonal IgG of the CSF could not be demonstrated in the SMA-positive patients by the methods used.

Adult↗
PubMed1977

Oligoclonal IgG and free light chains in the cerebrospinal fluid of patients with multiple sclerosis and infectious diseases of the central nervous system.

Conventional and crossed immunoelectrophoresis were used to characterize oligoclonal gamma-globulin bands of the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) or subacute sclerosing panencephalitis (SSPE) or other infections of the central nervous system. Most gamma-globulin bands were identified as IgG, but some bands were identified as kappa or lambda, or both, free light chains, Bands of IgG showed various degrees of light-chain diversity, and individual bands appeared in many instances to be derived from more than one clone of cells. Sequential changes of the oligoclonal IgG were observed in SSPE but not in MS. Oligoclonal IgG was detected in sera from most patients with SSPE and some patients with MS.

Central Nervous System Diseases↗
PubMed1977

Oligoclonal measles virus-specific IgG antibodies isolated from sera of patients with subacute sclerosing panencephalitis.

Measles virus-specific antibodies isolated from sera of six patients with subacute sclerosing panencephalitis (SSPE) were all composed of oligoclonal IgG proteins, in contrast to the polyclonal IgG antibodies isolated from sera of healthy individuals. Periods of disease progression were associated with an increase in number and amount of oligoclonal IgG antibody proteins, and a decrease was observed in disease remission. A correspondence between the oligloclonal measles-specific IgG of serum and the oligoclonal IgG proteins of cerebrospinal fluid (CSF) occurred in each case. It is concluded that sera from patients with SSPE may be used instead of CSF as a source for the isolation of measles antibodies of restricted heterogeneity.

Antibodies, Viral↗
PubMed1977

IgG1 subclass restriction of oligoclonal measles virus-specific IgG antibodies in patients with subacute sclerosing panencephalitis and in a patient with multiple sclerosis.

A restriction to the IgG1 subclass was demonstrated for measles virus-specific IgG antibodies isolated from the sera of five patients with subacute sclerosing panencephalitis, from the cerebrospinal fluid of one of the patients, and from brain extract from a sixth patient. A predominance of IgG1 was also observed in measles antibodies isolated from the serum and brain extract of a patient with multiple sclerosis. Evidence is presented that the IgG1 restriction is associated with the occurrence of oligoclonal measles antibodies in these patients. A similar restriction to the IgG1 subclass was not observed in measles antibodies isolated from the serum of a measles-convalescent child or from pooled normal serum and IgG.

Antibodies, Viral↗
PubMed1977

Gel-precipitating measles virus antibodies in subacute sclerosing panencephalitis: relation to oligoclonal IgG proteins in the cerebrospinal fluid and serum, and the occurrence of two separate fractions of antibody to virus nucleocapsids.

Two to five separate gel-precipitating (GP) antibody activities to sonicated measles virus cell pack antigen were detected by double immunodiffusion and immunoelectrophoresis of sera and cerebrospinal fluids (CSF) from patients with subacute sclerosing panencephalitis (SSPE). The major GP activity was identified as antibody to measles virus nucleocapsids, and a weaker activity as antibody to the virus haemagglutinin. The specificities of the other activities were not determined. Evidence is presented that measles GP antibody activities of both serum and CSF from patients with SSPE are carried by IgG proteins of restricted heterogeneity. Two separate and differently charged homogeneous IgG proteins with antibody activities to partially different measles nucleocapsid antigens were detected in about half the patients. The combined use of agarose electrophoresis and measles GP antibody immunoelectrophoresis of serum and CSF proved helpful in establishing rapidly the specific diagnosis of SSPE.

Antibodies, Viral↗
PubMed1977

Local synovial synthesis of oligoclonal measles virus antibodies and of smooth muscle antibodies in a case of atypical rheumatoid arthritis.

A patient with atypical rheumatoid arthritis (RA) and local synovial synthesis of oligoclonal IgG in an arthritic knee joint is described. Measles virus-specific antibodies isolated from the synovial fluid (SF) were carried by oligoclonal IgG proteins but constituted only a fraction of the total oligoclonal IgG of the SF. Smooth muscle antibodies were markedly increased in the SF compared with the serum and were associated with an electrophoretically restricted fraction of IgG. The results indicate that a local synovial synthesis of measles virus-specific antibodies and of smooth muscle antibodies occurred within the affected joint in our patient.

Antibodies, Viral↗
PubMed1976

IgG1 subclass restriction of oligoclonal IgG from cerebrospinal fluids and brain extracts in patients with multiple sclerosis and subacute encephalitides.

Oligoclonal-IgG-containing cerebrospinal fluids (CSF) from patients with multiple sclerosis and subacute encephalitides were studied for IgG subclass distribution by immunoelectrophoretic and hemagglutination inhibition methods. The immunoelectrophoretic results indicated the presence of electrophoretically restricted IgG1 proteins in a number of CSF, compatible with an association between this IgG subclass and oligoclonal IgG proteins. The combined results indicated a greater dominance of IgG1 over other IgG subclass proteins in CSF than in matching sera. Similar results were obtained in experiments with brain extracts from patients with subacute sclerosing panencephalitis. The results differed from those obtained with normal CSF, where the distribution of IgG subclasses resembled that of the matching sera. It is concluded that the oligoclonal IgG of the CSF and brain in the patients studied belong mainly to the IgG1 subclass.

Brain↗
PubMed1976

Measles virus-induced migration inhibition in vitro of leukocytes from patients with multiple sclerosis.

The migration inhibition in vitro induced by measles virus material on leukocytes from 17 patients with multiple sclerosis (MS) was compared with that of 8 patients with various other central nervous system diseases and that of 12 healthy blood donors. Significant differences could not be demonstrated. The results do not indicate that MS patients' leukocytes show an altered behaviour to measles virus material in the migration inhibition test.

Adult↗
PubMed1976

Measles virus-induced migration inhibition of human leukocytes in vitro: an expression of cell-mediated immunity?

Attempts were made to establish measles virus-induced migration inhibition of human leukocytes as an in vitro test of cell-mediated immunity to the virus. Crude material from cell cultures infected with two different strains of measles virus was used as antigen in the capillary modification of the test. Both virus preparations induced migration inhibition. Incubation with puromycin indicated that the inhibition was dependent on protein synthesis, which has been regarded as a characteristic feature of an immunologically specific inhibition. However, no difference was found when the migration inhibition of leukocytes from donors without clinical and serological evidence of previous measles infection was compared with that of leukocytes from donors with such evidence. It is concluded that the migration inhibition induced by crude measles virus material does not necessarily measure cell-mediated immunity to the virus.

Adult↗
PubMed1976

Oligoclonal measles virus-specific IgG antibodies isolated from cerebrospinal fluids, brain extracts, and sera from patients with subacute sclerosing panencephalitis and multiple sclerosis.

Measles virus-specific antibodies were isolated from sera, cerebrospinal fluids (CSF), and brain extracts of patients with subacute sclerosing panencephalitis (SSPE) and multiple sclerosis (MS) by absorption with measles antigens and subsequent acid elution of the antigen-antibody precipitates. Electrophoretically homogeneous measles antibodies were isolated from CSF or brain extracts in five patients with SSPE and in five out of seven patients with MS. Homogeneous IgG antibodies were also demonstrated in the sera from all SSPE patients and from three of the MS patients. The antibodies isolated from various control sera and from pooled CSF were electrophoretically heterogeneous. The results support the concept of a local synthesis in the nervous system of oligoclonal IgG antibodies to measles virus in all patients with SSPE and in some patients with MS. In SSPE, most or all oligoclonal IgG proteins of the CSF or brain carry measles antibody activities. In MS, only part of the oligoclonal IgG appears to be associated with measles antibody activity.

Antibodies, Viral↗
PubMed1976

Interferon in the serum and cerebrospinal fluid in patients with multiple sclerosis and other neurological disorders.

The presence of interferon (IF) was investigated in serum and cerebrospinal fluid (CSF) from neurological patients. Significant titres of IF were found both in the serum and in the CSF in about half of the patients suffering from acute encephalitis and from multiple sclerosis (MS), but not in patients suffering from various non-inflammatory disorders in the central nervous system (CNS) or in the peripheral nervous system (PNS), and not in the serum of healthy blood donors. Significant IF titres in the CSF were regularly associated with significant titres in the serum, but the converse was not true. Interferon levels were not correlated to cell counts in the CSF; nor to concentration of IgG and albumin in serum and CSF; nor to presence of electrophoretically oligoclonal IgG patterns; nor to hemagglutinating and gel-precipitation antibodies against measles. IF levels were lower in the serum of patients having reduced serum/CSF ratios for measles antibody than those with normal ratios. The data may indicate that MS is linked to factors which induce IF production in the CNS.

Antibodies↗
PubMed1975

Relationship between measles virus-specific antibody activities and oligoclonal IgG in the central nervous system of patients with subacute sclerosing panencephalitis and multiple sclerosis.

The association between oligoclonal IgG and measles virus antibodies present in cerebrospinal fluid samples or extracts of brain materials from 9 patients with subacute sclerosing panencephalitis was studied after electrophoretic separation. In all samples there was a good correlation between presence of bands of IgG and occurrence of specific antibody activities. The dominating band of oligoclonal IgG appeared in all cases to represent antibodies against the nucleocapsid antigen. Bands of oligoclonal IgG with a certain antibody activity displayed a tendency to occupy a preferential position in the electropherogram. Antibodies against the hemolysin and the nucleocapsids had a relatively more cathodic position than antibodies against the hemagglutinin. A clear correlation between bands of oligoclonal IgG and measles virus-specific antibody activity was not found in tests with materials from 4 patients with multiple sclerosis and 1 patient with myelopathy of unknown cause. However, a heterogeneous distribution of different antibody activities in separated material suggested that some electrophoretically restricted IgG proteins might carry these activities.

Antibodies, Viral↗