PubMed Health⌕ Search

Biomedical subjects

C Desaive

Publications and source records attributed to C Desaive.

At least 55 records · Page 3Linked to original sources
PubMed1982

[Relation between immunogenicity and differentiation of metastasis in an experimental murine melanoma].

The graft into the footpad of Jackson mice of B16 melanoma cells which are only slightly pigmented, induces the simultaneous appearance of achromatic and well-pigmented metastatics. We have analysed histologically both types of metastases from the 8th to the 20th day after the resection of the primary tumor. The percentages of mitoses are higher in the achromatic metastases than in the pigmented ones. Both are infiltrated by lymphocytes and by a small number of macrophages and polymorphonuclears. A statistical analysis demonstrates however that the percentage of lymphocytes is not related to the importance of the metastase pigmentation. The discussion is devoted to the relations between the infiltration by lymphocytes, the immunogenicity and the differentiation of the tumor.

Animals↗
PubMed1980

FPi analysis. II. Use of the method to monitor the in vivo kinetics of cell populations perturbed by hydroxyurea.

In a previous report, we described a new method called FPi analysis to analyze time sequences of DNA histograms taken from a perturbed population of cells. In this paper we utilize the method to analyze the in vivo kinetic response of bone marrow and of lung metastases of the B16 tumor to various chemotherapeutic agents. We show that the technique allows useful kinetic data to be obtained with minimal processing of the raw histograms, thus allowing fast analysis of the data. We also show that, in order to monitor the kinetic response of living tissues, it is essential to collect multiparameter distributions; to monitor only the one dimensional fluorescence histogram can give rise to misleading results. Using these multiparameter histograms, we are also able to monitor the growth fraction of the lung metastases during treatment, allowing discrimination between cell synchrony and cell recruitment from the resting compartment.

Animals↗
PubMed1979

Early detection of micrometastases via flow microfluorimetry.

We show that by measuring the joint distribution of fluorescent and scattered light in a suspension of lung taken from the C57B1/6J mouse, we are able to discriminate in vivo between the lung metastases of the B16 melanoma and the normal lung tissue. We can in such a way detect metastatic cells in a very early stage of growth and also obtain growth curves for the metastatic population. We analyze the sensitivity of the method of detection and speculate how it might be used in aiding in human diagnosis.

Animals↗
PubMed1978

Size dependence of cell cycle parameters for lung metastases from B16 melanoma.

Data presented show that the cell cycle time of the lung metastases from a B16 melanoma tumor growing in the foot pad of C57 BL/6J mice increases as the size of the metastases increases. This increased cell phase durations of the small metastases being closer to those of the primary tumor. The growth fractions of the primary tumor and of the lung metastases of different sizes are analyzed.

Animals↗
PubMed1977

[Comparison of the effects of ethidium bromide and of the ethidium bromide-deoxyribonucleic acid complex in Ehrlich tumor cells].

When injected into the peritoneal cavity, ethidium bromide can strongly inhibit the multiplication of mouse Ehrlich ascites tumour cells. This antitumour effect is increased when ethidium bromide is linked to DNA and also injected into the peritoneal cavity. The cellular alterations are identical after a treatment with E.B. either free or bound to DNA. However, when the cells are treated with E.B.-DNA they contain E.B. for a longer period than after a treatment with E.B.

Animals↗
PubMed1977

Experimental results on mammalian cells growing in vitro in deuterated medium for neutron-scattering studies.

SV-40 virus-transfromed human diploid fibroblasts (2RA) were grown in a monolayer on plastic Petri dishes in an aqueous medium deuterated to different concentrations of deuterium oxide: 10, 20, 30, up to 60%. The cells must be acclimatized to concentrations higher than 20% D2O by stepping them from a lower initial concentration during their exponential growth. The increase of cell doubling time with increasing deuterium concentrations seems to correlate, at least at 20% D20, with an initial period of suspended cell growth (lag-phase), and is qualitatively similar to that previously reported for Escherichia coli.

Cell Division↗
PubMed1977

[Cancer of the breast: Interrelations betwpen the primary tumor and the axillary and internal mammary nodes].

In a series of 300 patients operated for a cancer of the breast the internal and external mammary chain have been removed. The extemporaneous analysis has enable us to verify and complete the data collected at the clincial exmination. By this mean, we have selected on objective criteria the most favorables cases for conservative surgery. The histological examination of the lymph nodes shows a stricking relation between the T. and the N. This confirms the clinical conclusions. Finally we have shown that the appreciation of the T is of the outmost importance to predict an eventual envolvement of the internal mammary chain.

Axilla↗
PubMed1976

[Effects of the cis-dichloro-diammino-platinum(II)-deoxyribonucleic acid on normal and cancerous cells].

The effects of cis-dichloro-diammino-platinum(II) (cis-Pt) bound to DNA have been compared to those of free cis-Pt in mouse Ehrlich tumour cells, in peritoneal macrophages and in chick embryo fibroblasts cultivated in vitro. Cis-Pt has no antimitotic activity anymore when linked to DNA. This would be due to the fact that free cis-Pt is not released from cis-Pt-DNA complex inside lysosomes.

Animals↗
PubMed1976

Physical-chemical characterization of living cells by laser-flow microfluorometry.

A rapid method for the laser-flow microfluorometry determination of nucleic-acid content per cell is presented. A frequency distribution of fluorescence is obtained from suspensions of living cells treated with ethidium bromide directly in their own medium (or calcium-magnesium-free Hanks' balanced solution). For a fixed number of cells, a frequency distribution of fluorescence is obtained as a function of the amount of ethidium bromide progressively added to the suspension until staturation. At any ratio of added dye per unit of DNA, histograms generated from cells stained with this method give results similar to those generated after fixation and staining by the Feulgen technique, both in terms of cell-cycle phases and ploidy-level determination. The present technique requires a minimal amount of material, is instantaneous, and is conducted directly on living cells. Furthermore, dye concentration-dependence studies of mean fluorescence per cell allow determination of association constant and binding process (primary and secondary) between the intact cell and ethidium bromide. Cells which have the same amount of DNA but vary in the amount of RNA and/or chromatin conformation (like G0 and G1) can then be distinguished.

Animals↗