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Biomedical subjects

D Richard-Lenoble

Publications and source records attributed to D Richard-Lenoble.

At least 109 records · Page 6Linked to original sources
PubMed1979

Circulating immune complexes and C3d in human parasitosis.

Using the Raji cell radioimmune assay, we found low levels of circulating immune complexes (IC) in a small percentage of patients with schistosomiasis and filariasis. C3d levels, measured by immunoprecipitation, were elevated in a large number of these patients, whereas complement levels were within normal limits. Proteinuria was not found in any of the 55 patients studied. Circulating IC or elevated C3d levels were not found in any of the 19 patients with hydatidosis. The increased C3d levels, apparently not related to circulating IC, may be due to direct complement activation by parasite antigens or to sequestered IC. The latter hypothesis appears more attractive because the highest levels of C3d were found in schistosomiasis whereas schistosome antigens were unable to activate complement in vitro.

Antigen-Antibody Complex↗
PubMed1978

Human hydatidosis: evaluation of three serodiagnostic methods, the principal subclass of specific immunoglobulin and the detection of circulating immune complexes.

Three serological methods were examined for the diagnosis of hydatid disease: indirect haemagglutination, immuno-electrodiffusion and radioimmunoassay. The sensitivity of the three methods was similar. The principal subclass of specific anti-hydatid immunoglobulin was IgG and high levels of specific anti-IgE were found in two out of the five patients studied. The hypothesis that false negative reactions seen in patients with clinically confirmed hydatidosis may be due to the presence of circulating immune complexes was explored. Immune complexes were demonstrated in only two out of 13 patients studied and these patients did not have negative sera. It is concluded that for a satisfactory serodiagnosis of hydatid disease the radioimmunoassay and immuno-electrodiffusion should both be used and that further work should be done on the purification of hydatid antigens to improve the sensitivity of the radioimmunoassay without loss of specificity.

Antibody Specificity↗
PubMed1976

[Trial routine dections of malaria. Experimentation of 1,500 blood donors].

The aim of our research was to look for specific malaria antibodies in a randomized sample of 1.500 blood donors. We used the indirect immunofluorescence technique. During this project we have noticed the lack of validity of the "the five years rule" usually followed in France to prevent transfusion malaria. We have also evaluated the help brought by an "oriented investigation" allowing firstly a selection of potentially dangerous donors, secondly, the use of their blood bottles according to serology results. This work finally shows that total security can only be achieved by systematic research on all blood donors.

Blood Donors↗
PubMed1975

[Recrudescence of imported malaria (apropos of 30 recent cases)].

30 cases of imported malaria were diagnosed in the Laboratoire Central de Parasitologie of the Pitié-Salpêtrière C.H.U. in less than five months. The circumstances of appearance and the conditions of the patients' stay, the difficulties of the clinical diagnosis of the primary invasion, the gravity of P. falciparum attacks and the therapeutic schedules are presented. The accent is placed on the deficiencies of correctly prescribed anti-malaria chemotherapy and on the importance of information and sensitization of travellers.

Adolescent↗
PubMedDate not supplied

Reduced Loa loa microfilaria count ten to twelve months after a single dose of ivermectin.

The action of a single dose of ivermectin (200 micrograms/kg) on 71 Gabonese patients with Loa loa microfilariae in the peripheral blood, and living in areas highly endemic for loiasis, has been evaluated. Ten to 12 months after treatment, 43 patients (63%) had no circulating microfilaria and the geometric mean peripheral blood microfilaria count had decreased by 88.6% (P < 0.02). Thus, a single annual dose of ivermectin can markedly reduce loiasis transmission.

Adolescent↗
PubMedDate not supplied

Specificity of the solid phase alkaline phosphatase immunocapture assay for the diagnosis of human Schistosoma mansoni infection.

The species specificity of the solid phase alkaline phosphatase immunocapture assay (APIA) for the immunological detection of human immunoglobulin G antibodies to the alkaline phosphatase of adult Schistosoma mansoni was evaluated. Sera from schistosomiasis patients from South America, West Africa, south-east Asia and uninfected control subjects were compared. Only the sera of patients infected with S. mansoni gave positive results. There was no apparent difference between 2 populations infected with S. mansoni, one from South America and the other from West Africa. The results with sera from various regions of West Africa were also indistinguishable. Although the APIA was not able to discriminate the geographical origin of the S. mansoni-infected subjects, the method appeared to be specific for S. mansoni and suitable for use in the immunodiagnosis of schistosomiasis mansoni, particularly in endemic areas where mixed infections of Schistosoma spp. occur.

Alkaline Phosphatase↗
PubMedDate not supplied

White piedra and Trichosporon species in equatorial Africa. I. History and clinical aspects: an analysis of 449 superficial inguinal specimens.

White piedra is a hair infection characterized by nodules composed of fungal elements which envelop the hair shaft. Classically, this infection was considered to be produced by an asexual yeast-like fungus, Trichosporon beigelii. At present, in accordance with studies carried out previously, this species is subdivided into six newly defined distinct species (T. asahii, T. ovoides, T. inkin, T. mucoides, T. asteroides and T. cutaneum), all belonging to the class Basidiomycetes. Although widespread, white piedra has not previously been described in tropical regions of Africa. The present study, carried out in Libreville (Gabon), an equatorial region of Africa, shows that the incidence of this infection is quite high (18% of 449 inguinal specimens) in the female population aged 15-60 years, with a predominance in young patients (15-44 years). The relationship between the clinical manifestations and the specific hair lesions is also detailed. Three species belonging to the genus Trichosporon were identified: T. mucoides, T. asahii and T. inkin.

Adolescent↗
PubMedDate not supplied

White piedra and Trichosporon species in equatorial Africa. II. Clinical and mycological associations: an analysis of 449 superficial inguinal specimens.

Eighty-one of 449 Gabonese female patients examined were found to be positive for genitopubic white piedra. The association with trichobacteriosis is frequent (53 cases), and mostly seen with inguinal intertrigo. Fifty-two strains belonging to the genus Trichosporon were isolated from genital hairs as well as from inguinal intertrigo lesions. These strains were identified in accordance with previously defined morphological and biochemical criteria. Three species were recognized: T. mucoides (25 strains), T. inkin (20 strains) and T. asahii (seven strains). Their macroscopic and microscopic morphological properties, as well as their ability to reduce tetrazolium, were determined. In addition, the study of the clinical and pathogenic associations in which each of these strains was involved revealed some of their particular properties.

Adolescent↗