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G D Pegrum

Publications and source records attributed to G D Pegrum.

At least 73 records · Page 4Linked to original sources
PubMed1971

Platelet adhesiveness after blood donation.

Platelet adhesiveness to glass was measured in healthy blood donors at the time of and eight days after donating 500 ml of blood. By a whole blood method a highly significant increase was found whereas by a method using platelet-rich plasma with added adenosine diphosphate there was only a slightly significant increase. The discrepancy suggested that changes in the red cell population might influence the results. Packed red cells from 19 blood donors obtained at the time of donation and eight days later were mixed with fresh pooled platelets from the same independent persons on each occasion. The whole blood platelet adhesiveness on this mixture showed an increase in every case after blood donation. It is postulated that the increased adhesiveness is influenced by the presence of young red cells.

Adenine Nucleotides↗
PubMed1970

Estimation of platelet adhesiveness on whole blood and platelet-rich plasma.

Platelet adhesive tests were carried out in parallel on whole blood and platelet-rich plasma. A column of glass microspherules was used and adenosine diphosphate (ADP) was added to the platelet-rich plasma before passage through the column. Preliminary observations were made to show the effect of the containers and the anticoagulant used. The influence on platelets when stored at 4 degrees C and standing or mixing at room temperature was also assessed. The apparatus used to measure adhesiveness was designed to produce a constant rate of flow through a standard column of glass microspherules. Using both methods, adhesiveness was studied on 70 healthy blood donors. The result showed a significant correlation between the level of adhesiveness found with each technique.

Adenine Nucleotides↗
PubMed1968

Value of estimating methylamalonic acid excretion in anaemia.

A rapid technique suitable for routine pathology laboratories has been used to estimate methylmalonic acid excretion in a 24-hour urine collection following a 10g. valine load. Levels above 40 mg./24 hours were found only in patients with vitamin B(12) deficiency. Patients with pernicious anaemia treated more than 24 hours before urine collection and patients with other types of anaemia had methylmalonic acid levels below 25 mg./24 hours.This method of demonstrating vitamin B(12) deficiency can be applied rapidly in debilitated patients so that specific treatment can be instituted within 36 hours of admission.

Adult↗