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G Grosse

Publications and source records attributed to G Grosse.

At least 73 records · Page 4Linked to original sources
PubMed1986

[Scanning electron microscopy and light microscopy studies on the retina of chick embryos in cell culture].

Histological and scanning electron microscopy studies (SEM) suggest a classification of neural elements into five main types: receptor cells, bipolar cells, horizontal, amacrine, and ganglion cells in dissociated cell cultures of the retina from 7 to 10 days old chick embryos. By use of SEM the development of the receptor cell inner segments was observed. At the same time many protrusions were noticed at the receptor cell surface. Specific synaptic contacts between the axons of the receptor cells and the dendrites of the bipolar neurons, as well as unspecific contacts between the pericarya of the receptor cells and other dendrites were demonstrated. The bipolar neurons showed smooth cell surfaces, however, the horizontal cells appeared rough on the cell surfaces. Müller cells were always found adjacent to the photoreceptor cells.

Animals↗
PubMed1985

[Peracute disseminated course of fatal Aspergillus fumigatus infection in liver failure and corticoid therapy. A case report on the epidemiology, pathogenesis and diagnosis of the systemic course of Aspergillus infections].

Cytostatic-treated persons and cases of severe hepatic failure under corticoid therapy are predisposed to disseminating Aspergillus infections. Constant exposure to Aspergillus spores may result in a fatal Aspergillus infection. The triad of hepatic failure, corticoid therapy and constant exposure to Aspergillus spores is described in a 70-year-old female patient. A painless icterus was clinically diagnosed as non-A non-B hepatitis, with a protracted cholestatic course. She had been treated with an oral corticoid preparation. After leaving the hospital at her own insistance when still in the icteric stage, severe pneumonia due to Aspergillus developed within 14 days; this was confirmed radiologically. The autopsy results showed unexpected infarction, similar to pneumonic foci, in all lobes and dissemination in the myocardium, stomach, kidneys and brain. The liver showed subacute dystrophy. Constant exposure to the conidia of A fumigatus came about as a result of the soil of potted ornamental plants in the patient's living room. The fungus could only be successfully cultured by putting infected tissue particles on Sabouraud dextrose agar; it was not possible by the common method of fractionated streaking.

Aged↗
PubMed1985

[Diagnostic problems of virus-induced atypical squamous cell proliferations in the area of the anus and genitalia. A case report on Bowenoid papulosis].

Papillomatous proliferations of squamous epithelium of the genital and anal region termed Bowenoid Papulosis (BP) macroscopically resemble condylomata acuminata, whereas histologically they show in part severe dysplasias. Despite the similarity of these lesions to Bowen's disease they are - like condylomas - induced by papilloma viruses and may regress spontaneously. Condyloma-like but predominantly flat proliferations of squamous epithelium of the transitional zone between exocervix and endocervix, which are likewise caused by viruses and have a prognosis similar to that of BP, are even more problematic in differential diagnostic differentiation from cervical carcinoma in situ. Both these atypical proliferations of squamous epithelium of the exocervix referred to as "flat condylomas" and BP contain koilocytes as a sign of cell regression relatively typical of virus-induced cell change. In a reported case of a 38-year old woman with widespread BP of the ano-genital region intranuclear papilloma virus antigen could be demonstrated immunohistochemically in the papillomatous proliferations of squamous epithelium and furthermore in sections made from the filed paraffin blocks of a cone biopsy taken five years ago. In two of altogether seven cases of BP presented in tables papilloma virus antigen could be shown as well. In one woman of already 54 years of age widespread BP is described more comprehensively, because due to the feature of inverted papillomatosis carcinoma was suspected clinically. BP and flat condylomas have been defined as terms only in recent years and - as virus-induced lesions - been differentiated from carcinoma in situ on account of a more favourable prognosis.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1985

[Experimental ocular cryptococcosis under the influence of time-limited kidney failure--a pathohistological study].

This animal experiment is characterized by a missing virulence of Cryptococcus neoformans strain A94 (isolated from bird manure) for the immunocompetent mouse after intraperitoneal injection and following hematogenous dissemination into all organs including the central nervous system (CNS) and by the induction of a renal failure of short duration by intramuscular injection of glycerine. In 50% of the group of 80 animals damaged in such a way ocular cryptococcosis was found in association with the selective involvement of the CNS. The involvement of the eyes was dependent on a time-limited interval between the date of infection and the date of induction of the renal failure. The partly extensive pathohistological findings in the eye, with a predominance of mycotic processes in the retina and with some cases of endophthalmitis, were found to be similar to those described in man. The animal experiment described is proposed as a model for experimental studies to resolve problems of the immunology and therapy of the ocular cryptococcosis which are of current interest.

Acute Kidney Injury↗
PubMed1983

[Functional evaluation of islet cells following pancreatic duct occlusion--a long-term study].

The transduodenal occlusion of the pancreatic duct with Prolamin (Ethibloc) was carried out in an experiment with 38 Göttinger minipigs. Occlusion of the pancreatic duct leads to selective atrophy of the excretory pancreatic parenchyma. Up until now it has not been clear whether the endocrine functional capacity of the gland stays intact in a long-term experiment or whether the islands of Langerhans are also altered by the pancreatic duct occlusion. The following tests were carried out by us to determine the endocrine status: 1. Intravenous glucose tolerance test (i.v. GTT); 2. Glucose assimilation analysis after CONARD (K-value); 3. Insulin analysis during i.v. GTT; 4. Intravenous Tolbutamid test. Twelve months after the occlusion, reduced glucose tolerance and a reduction in insulin secretion were detected in the intravenous glucose tolerance test. Glucose assimilation (K-value) was not changed significantly. Fasting blood sugar and basic insulin level remained unchanged. Only a small blood-sugar decrease was measured after selective B-cell stimulation with Tolbutamid. The fibrosclerosis produced by the occlusion of the pancreatic duct with Prolamin also seemed to attack the cells of the islands and lead to their partial destruction.

Animals↗
PubMed1983

[Morphological studies of the effect of substance P partial sequences on nerve tissue culture].

Explants of the telencephalon and of the ganglion trigeminale from chick embryos were cultivated in the presence of 10(-7) M of substance P partial sequences SP 1-2 (Arg-Pro . 22HCl) and SP3-4 (Lys(Z)-Pro . HCl or Lys-Pro . 2HBr). The morphology of the living outgrowth in fact the growth of nerve fibers, cell migration and proliferation was observed. SP1-2 and SP3-4 influenced particularly the morphology of pns cultures.

Animals↗
PubMed1983

Pulmonary and extrapulmonary manifestations of L. pneumophila.

In three of five fatal cases of Legionnaires' Disease (LD) bacteria have been detected by DFA in extrapulmonary organs including spleen, liver, kidney, heart and brain. Typical fluorescent pleomorphic rods were seen mostly intravascularly and occasionally in phagocytes of the mononuclear phagocytic system. Legionellae were found extravascularly in the myocard and in the brain tissue together with single cell necrosis of either muscle fibres or ganglion cells in the respective organ. It was of special interest in this context that the clinical data and functional abnormalities reported for the respective cases might well be explained by the histopathological findings.

Adult↗
PubMed1983

Isolation of sporothrix schenckii from the floor of an indoor swimming pool.

In a routine mycological examination of an indoor swimming pool during the winter season (January 1981) in Berlin (West), Sporothrix schenckii was isolated from the floor near a shower. The strain was characterized by cultural, morphological and biochemical properties typical of strains which are causative agents of sporotrichosis. It is suggested that in routine mycological examinations of swimming pools attention should not be limited to dermatophytes and yeast-like fungi.

Animals↗
PubMed1982

Successful treatment of pulmonary and cerebral aspergillosis in an immunosuppressed child.

A favourable outcome was observed in a 12 year-old boy who developed invasive pulmonary and cerebral aspergillosis during antineoplastic treatment for central nervous system relapse of acute lymphoblastic leukemia. Combination therapy with amphotericin B and 5-Fluorocytosine led to complete regression of pulmonary infiltrates. Despite enlargement of the cerebral lesion monitored by computerized tomography, no viable fungi were found in the completely resected abscess after a 4 weeks' course of antifungal treatment preceding neurosurgery. Histological examination confirmed the diagnosis of an aspergillotic abscess. The initially severe neurological symptoms disappeared after successful surgery. Aspergillus fumigatus was detected in the soil of a potted ornamental plant in the mother's living room, suggesting that this might have been the source of the infectious agent.

Amphotericin B↗
PubMed1982

[Electron microscopic and morphometric studies on the in vitro differentiation of mitochondria in neurons of hippocampus explant cultures as affected by orotic acid and sodium orotate].

The influence of orotic acid and sodium orotate on the differentiation of mitochondria of the neurons within explantate cultures of the hippocampus of 18 day old rat embryos was investigated by means of morphometrical and stereological methods. The ultrastructure of the mitochondria in controls and pharmacologically influenced cultures was analysed on electronmicrographs after 3, 13 and 20 days in vitro. According to their morphological appearance and state of preservation the mitochondria were classified in 3 types: juvenile mitochondria (stage I), active mitochondria (stage II) and destroyed mitochondria (stage III). After 3 days of cultivation the neurons in cultures treated with orotic acid and sodium orotate showed significantly higher rates in the numerical density of mitochondria (ratio of the number of all mitochondria per unit volume of cytoplasm) in comparison with controls. Moreover, sodium orotate treatment lead to a significant increase especially in the numerical density of the mitochondria of stage II (active mitochondria) on the third day in vitro. However, the mitochondrial volume per unit volume of cytoplasma of the neurons in drug influenced cultures is lower than in controls for each of these dates--3., 13., and 20. day in vitro. The volume of the mitochondria increased under treatment with sodium orotate from the 3. up to the 20. day of cultivation but never reached the values of the uninfluenced controls. The plastic changes of the mitochondria due to pharmacological treatment with RNA--precursors demonstrate the possibility of a stimulating effect of the drug on the differentiation of neuronal mitochondria in vitro.

Animals↗
PubMed1982

[The effect of substance P (SP) and SP partial sequences on nerve fiber growth in tissue culture].

Explants of the ganglion trigeminale (PNS) and of the telencephalon (CNS) from chick embryos were cultivated in MAXIMOW chambers in semisynthetic media in the presence of dipeptide fragments (Lys(Z)-Pro . HCl, Lys-Pro-2HBr, Arg-Pro-2HCl) and the heptapeptide (SP5-11) of substance P as well as the complete substance P (SP1-11). 1. Histological examination of the dipeptide-treated CNS explants indicates that the structure of outgrowth in vitro is changed. Fascicel were observed. A stimulation of nerve fibre extension did not take place. 2.1. In dipeptide-treated PNS cultures the index of areas covered by the explants increased. 2.2. The index of nerve fibre growth increased significantly. The stimulation was caused in multiplication of fibres. Only Lys(Z)-Pro . HCl presents a prolongation of neurites. 2.3. SP5-11 effects in no case the growth of nerve fibres. SP1-11 stimulated significantly the fibre regeneration. 3. The possible role of SP1-11 with different effects under in vitro conditions is discussed. Only the N-terminal dipeptides stimulate the growth of nerve fibres. The C-terminal SP5-11 is without effect. Finally it is stated that the best results in neuritic enlargement and neurogenesis can only be obtained by cultivation with SP1-11.

Animals↗
PubMed1982

[Morphometric studies of the rat hippocampus after static and dynamic cultivation].

Explants of the hippocampus (rat) were cultivated either in maximow chambers without movement (static) or in movement (dynamic) by rotation in roller tubes. The growth of nerve fibres, the density of cells (matrix, neurons) and the area of cell nuclei was observed. The determination of the index of nerve fibre growth, the procedure for counting cells in a tissue with different densities and the value of the two methodological approaches of cultivation is discussed. Finally it is stated that best results in morphology of explants can only be obtained by cultivation without movement.

Animals↗
PubMed1982

[Effect of the culture technic on the development of the hippocampus (rat) in vitro].

150 hippocampal explants from fetal and neonatal rats were cultivated with semisynthetic media in maximow chambers without movement and in roller tubes by rotation in the virutor up to 3 days. The cultures were fixed in Bouin, cuted 15 microns and coloured with the method of Klüver-Barrera or haemalaun. Histological examination of the explants revealed that hippocampi cultured in maximow chambers without movement possess an organisation similar to that observed in situ. Experiments with rotated explants of hippocampus indicate that the structure of hippocampus in vitro is changed. Especially neurons are degenerated and the migration of cells is altered. Finally the value of these two methodological cultivations for neurobiology is discussed.

Animals↗