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Biomedical subjects

G Müller

Publications and source records attributed to G Müller.

At least 577 records · Page 32Linked to original sources

[Adenosine deaminase activity in Hodgkin's disease (author's transl)].

The activity of adenosine deaminase (ADA) (EC3.5.4.4.) was determined in the blood plasma, erythrocytes, and lymphocytes of 23 patients with Hodgkin's disease and partly also in 99 control subjects. The enzyme activities were measured using adenosine as substrate and by analysis of ammonia. No correlation was found between the ADA activities in lymphocytes, erythrocytes, and blood plasma. The lymphocytes of the patients revealed lower ADA activities (U/g protein) than the lymphocytes of control subjects. The ADA activity is not reduced in plasma or erythrocytes. The lower activities of ADA in the lymphocytes of patients may be related to the impaired cell-mediated immunity of the Hodgkin's disease.

Adenosine Deaminase↗

Monoclonal antibody (Tü48) defining alloantigenic class I determinants specific for HLA-Bw4 and HLA-Aw23,-Aw24 as well as -Aw32.

A monoclonal antibody (Tü48) was prepared against human lymphocytes. Immunochemical analysis indicated that Tü48 binds to a fraction of HLA molecules. Tü48 was tested on 398 HLA-A,B,C/DR-typed normal blood donors in the microcytotoxicity assay and was found to detect a "supertypic" determinant on molecules bearing the HLA specificities -Bw4, Aw23(9), -Aw24(9), and -Aw32. Among the HLA-Bw4-positive individuals, negative or weak reactivity of Tü48 was found with about one-fourth of normal donors heterozygous for the HLA antigen Bw44. This points to the possibility that the antigenic determinant detected by Tü48 is not expressed on all HLA-molecules carring the HLA-Bw4 specificity on the cell surface. Alternatively, weak or no expression of the Tü48 antigenic determinant on some HLA-Bw44 bearing molecules might be explained by the existence of molecular variants of glycoproteins with this HLA specificity. The concept of "supertypic" HLA specificities is discussed with regard to the expression of a monoclonal antibody-defined epitope on both certain HLA-A and B molecules.

Animals↗

Fascioliasis problems in ruminants in Rio Grande do Sul, Brazil.

The economic importance of fascioliasis in the livestock industry in the state of Rio Grande do Sul was assessed through the Federal meat inspection at major slaughter houses. Annual average rates of the livers condemned by Fasciola hepatica infection at the houses in the state have been recorded as 12-13% in cattle and 7% in sheep, respectively. Many farms in the provinces of the frontier regions of Brazil with Uruguay are contaminated with F. hepatica and acute fascioliasis with a high mortality among sheep flocks frequently occurs during the wet and cold winter season in the lowland areas of the regions. The two Lymnaeid snails, Lymnaea viatrix and L. columella are distributed in the state and act as intermediate hosts of F. hepatica. The former species, called the "mud snail", is more predominant than the latter: "pond snail".

Animals↗

[Special indications for the use of the Grüntzig balloon catheter in surgical problem cases].

The excellent experiences with the intravascular application of Grüntzig's double-lumen balloon catheter shows his potential use in thin-walled organs. We report our experiences with the dilatation of bile-duct stenoses (end-to-end anastomosis of the Ductus choledochus, sclerosis of the Papilla Vateri, intra- and extrahepatic fibrotic stenosis), stenosis of the esophagus after acid corrosion and stenosis of the ureter anastomosis after Anderson-Hynes operation. These results demonstrate, that in selected cases the balloon dilatation may be an alternative to surgical intervention.

Aged↗

Albumin in chicken skeletal muscle.

Albumin was found to be a structural constituent of chicken myofibrils. Its subcellular localization as shown by electron microscopy demonstrated highest concentrations of albumin in the region where the thin and thick filaments overlap. In addition, evidence is presented in favour of albumin synthesis in muscle. These results suggest a physiological role of an albumin in muscle, most probably in the fatty acid metabolism.

Albumins↗

Histological, proliferative, and biochemical alterations in dorsal epidermis of the Syrian golden hamster induced by 12-O-tetradecanoylphorbol-13-acetate and the calcium ionophore A 23187.

Long-term treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) of dorsal skin of 7,12-dimethylbenz(a)anthracene-initiated Syrian golden hamsters does not lead to the formation of epithelial tumors and leaves the epidermis essentially unchanged. However, previous histological studies by others have shown that hamster epidermis can be hyperplastically transformed by a single application of TPA but that the tissue is capable of gradually adapting to the drug after extended TPA exposure. We have investigated the response of hamster back epidermis to single and multiple treatments with increasing doses of TPA with regard to histological, proliferative, and biochemical alterations, and we show that in our animal strain the dorsal epidermis is resistant to even a single exposure to TPA, although the clearance rate of TPA is comparable to that in mouse epidermis and the metabolism of the substance is negligible. In contrast, the epidermis could be moderately stimulated by a single application of the nonpromoting calcium ionophore A 23187 and exhibited a strong proliferate and hyperplastic response following the simultaneous exposure to the calcium ionophore and TPA. Both types of hyperproliferation did not reveal an initial depression of the proliferative activity and were accompanied by typical alterations of the keratin polypeptide pattern, which was not detectable after treatment with TPA alone.

Adaptation, Physiological↗

[Drug induced nephropathy and enzymes of renal origin in the urine].

A report is given on the excretion of the renal enzymes alaninaminopeptidasis (AAP), N-acetyl-beta-D-glucosaminidasis (NAG), beta-glucoronidasis (GBC) and alpha-galactosidasis (GAC) in urine after administration of Gentamycin, Cephalotin, Dextran, Carbencillin or combinations of these medicaments. Moreover the effective level of Gentamycin in the urine was measured after isolated administration of Gentamycin. A rhythmic excretion of ciliated-border and lysomal enzymes was observed. Gentamycin was also rhythmically excreted with the urine. It is assumed that this reflects cyclic changes in the cells of the renal tubule. Under long-term administration of Gentamycin or Dextran the excretion of enzymes already reduced during therapy. This was interpreted as an adaptation of the kidney to the medicament. On the other hand there are combinations of medicaments whose effect on the epithelium of the renal tubule is potentiated.

Acetylglucosaminidase↗

[Economic problems and contradictions in mineral fertilizer application for solving nutrition problems in the developing countries].

The optimum application of mineral fertilizers in the agriculture of the developing countries is inhibited by the monopolization of the mineral fertilizer industry in the capitalist economic world system and the numerous contradictions in the developing countries themselves. Thus it requires the full state sovereignty and economic independence of the developing countries, and the creation of such social conditions which enable the further extension and better utilization of the fertilizer capacities as well as the optimum fertilizer application. This means applying mineral fertilizers in the best possible way in order to ensure the nutrition of their own population and aiming at agrarian structures which promote mineral fertilization, restructuring the system of mineral fertilizer prices, and developing the mineral fertilizer service.

Developing Countries↗

[The intravenous fat tolerance test in patients with peripheral arteriosclerotic disorders of the blood supply].

Latent metabolic disturbances become evident only by specific loads. As functional test of the fat metabolism the intravenous fat tolerance test is suited in which after an injection of 0.1 g fat/kg body weight via the decreasing plasma turbidity the degradation rate K2 (%/min) may be calculated. In 29 patients with peripheral arteriosclerotic disturbances of the blood supply we did not find any changed degradation rates in comparison to 29 reference persons, so that a disturbance of the clarification system is not to be supposed in these patients. It is reported on the behaviour of other metabolic parameters. At the same time by the slight changes in the intermediary metabolism after fat injection the good tolerability of the emulsion of soya bean oil lipofundin S which is known from clinical experience is supported.

Arterial Occlusive Diseases↗

[Is the intraoperative electrical stimulation test of Burge indispensable in order to ensure complete denervation in selective proximal vagotomy? (author's transl)].

The success of selective proximal vagotomy in the treatment of chronic duodenal ulcer is dependent on complete denervation of the proximal part of the stomach, as well as on the preservation of antral innervation. Inadequate nerve dissection results in therapeutic failure. The most frequent cause of recurrent ulcers is incomplete vagal denervation of the acid-secreting cells. Therefore, it would seem desirable to use an intraoperative test to check completeness of vagotomy, giving the surgeon the possibility of rectifying an inadequate nerve section still at the time of operation. Among several methods the electrical stimulation test described by Burge and Vane has gained general acceptance. However, the question of whether the intraoperative test actually improves the results of vagotomy remains equivocal. In an initial series of 39 vagotomies we ensured completeness of denervation by the electrical stimulation test. In a subsequent series of 44 operations vagotomy was performed without the test. The patients were re-examined one and again two years following operation (clinical evaluation, gastroscopy and/or X-ray examination). The results of this retrospective analysis using the classification of Visick showed no difference between the two series. Hence, we have no reason to suppose that omission of the intraoperative test will result in an increased number of incomplete vagotomies, provided the surgeon has gained his experience in this operative procedure by using the test to check his surgical technique, which thereby becomes standardized. Thereafter, the Burge test becomes dispensible.

Duodenal Ulcer↗

[Clinical value of N-acetyl-beta-D-glucosaminidase and alpha-galactosidase in liver diseases].

N-acetyl-beta-D-glucosaminidase is a lysosomal glycosidase, which participates in the catabolism of the mucopolysaccharides and of the glycoproteins. For the determination of this enzyme the production of the substrate and a modified methodology are described. Measurements of the activity were performed by 4-nitrophenyl-alpha-D-galactopyranoside and 4-nitrophenyl-N-acetyl-beta-D-glucosaminide as substrates. Examinations of this enzyme were performed in patients with acute virus hepatitis, chronic hepatitis, liver cirrhosis and healthy test persons. In acute hepatitis and liver cirrhosis and significant increase of enzyme is provable, but not in protracted hepatitis.

Acetylglucosaminidase↗