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Biomedical subjects

H Tchórzewski

Publications and source records attributed to H Tchórzewski.

At least 109 records · Page 6Linked to original sources
PubMed1987

Interaction of lysosomal enzymes and their natural inhibitors in bronchoalveolar lavage fluid and serum in atopic bronchial asthma.

To evaluate the role of proteolytic balance in atopic bronchial asthma, the activity of lysosomal enzymes and the concentration of their natural inhibitors were determined in bronchoalveolar lavage fluid and serum in 28 patients with asthma. The results were statistically analyzed. A significant increase of lysosomal enzyme activities as compared with healthy volunteers was detected mainly in bronchoalveolar lavage fluid. Similarly, a selective increase in the concentration of the natural inhibitors of proteolytic enzymes, especially alpha-2-macroglobulin, was noted. Moreover, the concentrations of alpha-1-antitrypsin and the activities of neutral protease, alpha-1-antitrypsin, alpha-2-macroglobulin and acid protease were correlated. These findings suggest the participation of lysosomal enzymes and their natural inhibitors in immune response during atopic bronchial asthma. The protease antiprotease balance control may be used in prognostic and therapeutic procedure in diseases of the respiratory tract.

Acid Phosphatase↗
PubMed1987

Disorders of the lymphatic homing and MIF production by the lymphocytes of thymus, spleen and lymph nodes after liver injury caused by portocaval shunt in rats.

This paper presents the quantitative changes and disorders in migratory inhibition factor (MIF) production by thymus, lymph node and spleen cells after liver injury provoked by the porto-caval shunt (PCS) in rats. The great depopulation of the lymphatic organs, particularly the thymus, was demonstrated. The reduction of MIF generation after PCS was also noticed. The severe liver injury reflected by high activity of GOT and GPT was observed on the second day after surgical procedure. Our investigations bring into light the multifold interrelationships between the functionally efficient liver and lymphatic organs in rat.

Animals↗
PubMed1985

Changes in the interleukin-1 and interleukin-2 generation in duodenal ulcer patients during cimetidine treatment.

The effect of cimetidine treatment on the generation of interleukin-1 (IL-1) and interleukin-2 (IL-2) was studied in 11 duodenal ulcer patients. The results obtained were compared with those for untreated healthy subjects. The drug was administered intravenously in a dose of 200 mg four times a day for 8 days. The investigations were performed before, during and 1 wk after cimetidine therapy. IL-1 generation was determined by the ability of supernatants from 2-day cultured adherent cells stimulated by lipopolysaccharide to enhance proliferation of PHA-stimulated mice thymocytes. IL-2 generation was determined by the ability of supernatants from 2-day cultured, PHA-stimulated mononuclear cells to proliferate autologous 17-day cultured T cells. In all ulcer patients IL-1 generation diminished during cimetidine treatment (P less than 0.005). It continued to decrease in 4 subjects and increased in the other 7 ones following drug withdrawal. All the values were higher than those in healthy controls. IL-2 activity in ulcer patients was similar to that in healthy subjects and it increased significantly in all ulcer patients following the onset of the treatment (P less than 0.005) and decreased nearly to the initial values 1 wk after termination of the treatment (P less than 0.005). The present studies indicate that cimetidine, a selective histamine H2-receptor antagonist, deeply changes mechanisms of immunoregulation in patients with duodenal peptic ulcer.

Adult↗
PubMed1985

Analysis of proteolytic enzymes and their natural inhibitors in serum and bronchial lavage fluid in atopic bronchial asthma, chronic bronchitis and pneumonia.

The protease-antiprotease balance was evaluated in some respiratory tract diseases. Analysis of the protease activity and the natural inhibitors of proteolytic enzymes was carried out on 10 patients with atopic bronchial asthma, 21 with chronic bronchitis, 12 with pneumonia and 11 health volunteers. Paralelly, the inflammatory potency of bronchial lavage fluid was determined in guinea-pig skin test. In our studies of bronchial lavage fluids some selective changes of proteolytic enzyme activities were documented as follows: in pneumonia patients--the increase of acid and neutral proteases activity; in chronic bronchitis--the moderate increase of activities of all examined proteases and in atopic bronchial asthma--the increase of acid protease activity. Similarly, levels of the natural inhibitors of proteolytic enzymes were selectively elevated: alpha-1-antitrypsin in pneumonia and chronic bronchitis patients and alpha-2-macroglobulin in asthmatics. This finding may suggest that the proteolytic enzymes and their inhibitors play an important role in the respiratory tract pathology. This concept is supported by the high inflammatory response observed with bronchial lavage fluids obtained from pneumonia patients in guinea-pig skin test. The authors suggest that the determination of protease activity and level of the natural inhibitors of proteolytic enzymes in bronchial lavage fluids may be useful for clinical prognosis and pharmacological treatment.

Adolescent↗
PubMed1984

The effect of cimetidine treatment on suppressor T cells in duodenal ulcer patients.

Changes in the suppressor T-lymphocyte activity were studied in 11 patients with duodenal ulcer during treatment with cimetidine. The drug was administered intravenously in a dose of 200 mg four times a day for a fortnight. Suppressor T-cell activity was determined by the Shou et al. method using two-stage culture before treatment, after 4 days of the treatment, just before drug withdrawal, and 2 days and 2 wk after the treatment. Suppressor T-cell activity significantly decreased soon after starting the treatment, remained low throughout the treatment, and rapidly and significantly increased following drug withdrawal.

Adult↗
PubMed1984

Modulation of immune response in vitro and in vivo by human granulocyte factors.

The adherence of granulocytes induces secretion of specific granule contents. The secreted proteins were termed granulocyte factors (GF). The experiments in vivo provide evidence that GF play an essential role in the stimulation of PFC in BALB/c mice immunized with SRBC when applied before challenge three times (5 micrograms per mouse), but 50 micrograms per mouse given in the same way diminishes the response. To elucidate this discrepancy, the effect of GF on the generation of suppressor cells (SC) and helper cells (HC) in vitro has been investigated. Antigen specific nonadherent SC or HC were induced in vitro using CBA mice spleen cells incubated with 100 micrograms/ml or 0.1 mg/ml of TNP-KLH, respectively, for 4 days. GF in concentrations of 0.1 to 1 microgram/ml abolish antigen specific SC generation. SC and HC activity was tested in cooperative cultures. Antigen specific SC in delayed hypersensitivity (DTH) to BCG were induced in an in vitro system as above using normal BALB/c spleen cells and 100 micrograms/ml PPD. Nonadherent suppressor cells were transferred intravenously into cyclophosphamide (CY)-treated syngeneic recipients. The recipients were immunized to BCG immediately after the cell transfer. DTH was measured by foot-pad reaction. This reaction was positive to PPD in CY treated mice immunized to BCG, while it was suppressed by the transfer of in vitro induced SC. When the SC were induced in the presence of 1 microgram/ml GF, the suppression was abrogated. The higher GF concentrations stimulated SC activities when they were measured in response to a nonrelated antigen and in specific anti-PPD response, but the HC inhibition could not be excluded.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1984

Release of neutrophil granule factors during early period of hemodialysis: a possible cause of hemodialysis neutropenia.

In the early phase of hemodialysis progressive decreases in some enzyme activities in the leukocyte homogenate, neutrophil granule fraction and postgranular supernatant were found with concomitant rise in plasma beta-glucuronidase activity, which is indicative of the release of neutrophil granule factors into the extracellular environment. Intravenous infusion of human neutrophil granule products to rabbits induced profound transient neutropenia. The results suggest that the release of neutrophil granule factors in the early period of hemodialysis may be a possible cause of hemodialysis neutropenia.

Adult↗
PubMed1983

Factors influencing human leukocyte adherence in vitro.

Studies were performed on factors influencing leucocyte adherence in vitro. Blood condensation was found to increase leukocyte adherence. Addition of heparin, dextran or ethanol caused a significant reduction of white blood cell count in blood samples in comparison with blood mixed with sodium EDTA or ACD solution. This suggests the existence of two granulocyte subpopulations; viz, rapidly adhering and slowly adhering. Heparin enhanced granulocyte adherence, while dextran and ethanol decreased it. Five-day storage of ACD blood led to a decrease in granulocyte adherence, while addition of heparin or histamine to ACD blood prevented this change to occur. The glucose concentration of 1,000 mg/dl augmented granulocyte adherence, while higher glucose concentrations induced its progressive fall below the control values. There was no significant change of lymphocyte adherence during the experiments.

Adult↗
PubMed1983

Factors influencing human leukocyte adherence in vivo.

The effects of different factors on leukocyte adherence was evaluated by using human peripheral blood before and after treatment (application). Magnesium ions, anti-inflammatory drugs and dextran have decreased neutrophil adherence. Calcium ions, propranolol, blood transfusion caused a significant decrease in adherence. These findings support the conclusion that the adherence test, which is influenced by numerous factors and whose results had a wide scatter, is not a useful tool in clinical diagnosis.

Adult↗
PubMed1983

Comparative study on the immunosuppressive and lympholytic activity of optical isomers of cyclophosphamide.

The influence of chirality of cyclophosphamide (Cy) on the reactivity of lymphoid cells in vivo was studied in Balb/c mice. It has been shown that (+) Cy inhibits the formation of direct PFC in Balb/c mice more effectively than (-) Cy. The augmentation of DTH--reaction against HuRBC in mice, measured by the foot-pad test and the elongation of graft survival time were noted when racemic or (-) Cy were applied. Investigated forms of Cy decreased the number of nucleated cells in mice spleens, but lympholytic effect of (+) Cy was lower than that of racemic and (-) Cy. The possible consequences of stereo-differentiated immunosuppressive activity of enantiomeric forms of cyclophosphamide in the experimental procedures and the therapy of "autoimmune" and malignant diseases are considered.

Animals↗
PubMed1981

The effect of lysosome factors derived from PMNL of multiple sclerosis and systemic lupus erythematosus patients on suppressor cell activity in vitro.

The lysosomal factors obtained from PMNL of healthy persons and patients with multiple sclerosis, systemic lupus erythematosus and other neurological diseases inhibited in vitro the generation of Con A-induced suppressor cell activity. The results obtained proved that these factors from granulocytes of MS-relapse and active LE have a stronger inhibitory effect on suppressor cell activity than on healthy ones.

Adult↗
PubMed1981

The effect of polymorphonuclear leukocyte (PMNL) factors on T suppressor cell function in in vitro studies in healthy persons and some autoimmune disorders.

Lysosome proteins derived from peripheral blood granulocytes of patients with multiple sclerosis (MS), lupus erythematosus (LE), rheumatoid arthritis (RA) and recurrent uveitis cause the impairment of Con A-induced suppressor cell activity in two-step culture in vitro. This effect is independent of lysosome protease activity. Such a phenomenon, observed in vitro, may cause disturbances in suppressor cell function in the course of the above diseases.

Adult↗