Biomedical subjects
Publications and source records attributed to I Mikó.
For preservation of the pancreas, extracellular and intracellular solutions with aprotinin light microscopy showed both solutions to be suitable for a 3-hour preservation, though the intracellular solution appeared more suitable. Preservations for 6 and 24 hours have also been attempted, but these specimens showed fairly severe histological lesions.
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Autologous and allogenic transplantations of the caudal part of the pancreas into the omentum and the spleen were performed in 18 dogs. The fate of the transplants was followed histologically. In the grafts the Langerhans-islands preserved their integrity longer than in the other parts of the organ, but in 4 to 6 weeks they, too, necrotised.
After surveying the problem of pancreas transplantation the results of pancreatico-duodenal transplantation attempts performed on dogs and 20 pigs are reported. Surgically, the major pancreatic duct is anastomosed to a Roux' loop using a preserved duodenal patch around the papilla. The graft is sited unto the retroperitoneal space. The animals survived for 6 to 9 months. In some cases an increasing fibrosis of the transplantate was observed. Most often, the animals died from pneumonia. The authors want to continue their research in order to obtain better results.
Segment resection was performed in 15 dogs. The resected surface was covered with absorbable cellulose bioplast (Surgicel)R and fixed by the tissue adhesive Histoacryl-N-Blau. The results were checked by gross and microscopic examinations which showed that the gradually absorbed material had been replaced by a layer of connective tissue. The method is recommended for use in human operations.
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Forty experimental anastomoses have been prepared with the help of polyethylene tubes on the hearts of 10 human corpes, 2 pigs and 16 dogs. In dogs the left internal mammary artery was anastomized with one of the coronary branches of the moving heart without extracorporal circulation. The plastic tube was moved in the vessel by means of a thread attached to the end of the tube and the tube was retrieved by the same thread before the closure of one of the suture lines. The method is illustrated on schemiatic diagrams and its advantages are pointed out.
Large nephrotomy incisions made in 15 dogs were closed by sewing up the cavity system with stitches of 5/0 catgut and by sealing the renal parenchyma with tissue adhesive being pinpointed at the opened vessels. The incision was covered with a preparation of oxidized cellulose which was then likewise impregnated with tissue adhesive. This technique was found rapid and convenient and provided for adequate haemostasis. Its essential advantage lies in eliminating the deep through-and-through sutures involving a major loss of functioning renal parenchyma. All substances used were absorbed in due time.
Both kidneys of 12 dogs were injured and the wounds were covered with Surgicel which was fixed by means of adhesive. The results were checked by visual inspection and histological tests. The method seems to be suitable for use in appropriate human cases.
Experiments were carried out on 15 dogs to repair the parietal peritoneum with an auto-alloplastic structure whose internal smooth surface was turned towards the abdominal cavity. Gross and histologic examinations have confirmed adaptation of the implant to the new environment without the formation of adhesions.
End-to-end small intestinal anastomoses were performed in 20 dogs. In four dogs, the anastomosis was secured by distant single line stitches. These animals died of peritonitis. In the other animals the suture was covered with Surgicel and fixed by means of Histoacryl-N-Blau adhesive. In these cases, no insufficiency developed and in two cases were only adhesions and intestinal obstruction, respectively, observed. All the animals survived the operation. The method is recommended for securing intestinal anastomoses and stumps in patients.
Skin defects of full thickness were produced in 15 rabbits. The wounds were covered with Surgicel soaked in the animal's own blood and fixed at the edges with Histoacryl-N-Blau adhesive. A gradual epithelization took place under the covering layer. The method is suggested as an emergency measure in case of serious burns or other extensive skin injuries.
In 15 rabbits skin defects of various size were induced. The defects were covered with blood-soaked Surgicel which at the margin was attached with Histoacryl adhesive. Gradually under this cover the epithelization proceeds. The authors recommend this emergency procedure in severe cases of burns of other extended skin injuries.
A new method has been worked out for the treatment of experimental pancreas injuries and resections. The surfaces were coated with SurgicelR and the coating was fixed with the adhesive Histoacryl-N-Blau. Use of these two completely absorbable substances prevented the complications caused by sutures. The results confirmed by histological and laboratory tests have been satisfactory, hence the method appears to be clinically applicable.
Gastric, intestinal and hepatic injuries induced in dogs were treated with a collagen preparation and an adhesive. The animals survived the intervention and absorption of the materials applied proceeded parallel to the healing process.
Treatment of 28 stabbed heart wounds in 15 dogs was done with traditional suturing or by means of an adhesive and Surgicel. The combination of Surgicel with adhesive ensured adequate closure of the wound and healing. The method is recommended for the treatment of human heart injuries in certain cases.
Oesophageal rupture was induced in 18 dogs and the animals were divided into three groups according to the treatment of the wound. In the first group the wound was closed with the adhesive Histoacryl-N Blau and Surgicel, in the second group with the same adhesive and a collagen preparation, while the wounds of the third group was sutured. Because of the inadequacy of suturing half of the animals in the third group died, while in the other groups every animal survived the operation. The adhesive plus Surgicel provided the most satisfactory closure of the wound.
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