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J Musil

Publications and source records attributed to J Musil.

At least 37 records · Page 2Linked to original sources
PubMed1985

[Detection of lymphocyte subpopulations in EDTA whole blood samples using flow cytometry. Stability and normal values].

Series of T-cell-specific monoclonal antibodies (OKT 11 for peripheral T-lymphocytes, OKT 4 for helper/inducer T-cells, OKT 8 for suppressor/cytotoxic T-cells), one B-cell-specific antibody (anti-LEU-10) and the antibody anti-LEU-7 for LGL/NK-cells were used to detect the distribution of different lymphocyte subpopulations in the peripheral blood of 60 healthy, clinically examined test persons. The flow cytometry was performed by means of direct immunofluorescence method. The statistical evaluation showed that the results in all three age groups studied did not essentially differ with the exception of the so-called natural killer cells which increase significantly with advancing years. The described method allows the dispatch of lymphocytes samples even from places far away and the analysis of the specimen within 36 to 48 h.

Antibodies, Monoclonal↗
PubMed1984

Cytoprotection by somatostatin of normal and malignant clonogenic cells against the in vitro cytotoxicity of bischloroethylnitrosourea (BCNU).

The various pharmacological effects of somatostatin may be explained by the hypothesis that the paracrine peptide, by "stabilizing" cell membranes, inhibits the secretion of hormones as well as protects other cells (vascular endothelium, parenchyma) from different lesions (vasculo-, organo-, cytoprotection). This hypothesis was tested in vitro, using bischloroethyl-nitrosourea (BCNU)-intoxicated stem cells of normal mouse granulopoiesis and of the L 1210 leukemia. Clonogenic mouse bone marrow and L 1210 cells were grown in agar-containing glass capillaries. Using these colony assays and a ID90 of BCNU, cyclic somatostatin influenced the BCNU-cytotoxicity neither at simultaneous nor at subsequent application. However, when given 2 h prior to BCNU, the inhibition of colony growth was almost totally abolished. This cytoprotective effect was seen with normal granulopoietic as well as with leukemic cells. The effect did not show up, if the inactive linear somatostatin was used. N-acetyl-cysteine, a SH-compound applied as a chemoprotective adjunct, did not reveal a cytoprotective effect under identical experimental conditions, either. The results were discussed in view of common efforts to reduce the toxicity of cancer chemotherapy.

Acetylcysteine↗
PubMed1979

Metabolic turnover of pyridine nucleotides in ascites cells of sarcoma Sa 180 and in the liver tissue of rats before and after ionizing radiation.

Metabolic turnover of NADP+ labeled with 14C in the ribose moiety of their molecules was determined in the ascites cells of sarcoma Sa 180 and in the rat liver tissue. The half-lives of NAD+ and NADP+ in the Sa 180 sarcoma cells were 60 min (NAD+) and 90 min (NADP+) and 120 min (NADP+). Experiments were done on animals to study the time-dependent effect of ionizing radiation on the values of pooled NAD+ and NADP+ after 0.129 C/kg (500 R) and 0.387 C/kg (1500 R) whole-body irradiation, and the metabolic turnover of these nucleotides 5 h after whole-body irradiation with 0.387 C/kg (1500 R). Exposure to 0.129 C/kg (500 R) whole-body irradiation induced no apparent changes compared to the controls. Within 5 h of irradiation the whole-body dose of 0.387 C/kg (1500 R) produced changes in rat liver cells characterized by a reduction of the nucleotide biological half-lives (NAD+ from 80 to 60 min and NADP+ from 120 to 70 min). No such changes in the pyridine nucleotide turnover were detected in Sa 180 ascites sarcoma cells.

Animals↗