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Biomedical subjects

J Prokopowicz

Publications and source records attributed to J Prokopowicz.

At least 37 records · Page 2Linked to original sources
PubMed1977

Antihaemophilic factor (factor VIII) from human granulocytes.

Factor VIII has been isolated and purified from human granulocytes using chromatography on Sephadex G-200 AND DEAE-Sephadex A-50. The final preparation was purified 60-fold. Some properties of this partly purified factor VIII were compared with the human plasma AHF preparation. Optimum pH activity and the highest activity after incubation at different pH values was found in buffer at pH 7.0. The preparation was thermolabile and had a molecular weight of about 214,000. Absorption curves indicated that it is a protein exhibiting four fractions in polyacrylamide gel disk electrophoresis. AHF preparations from plasma and granulocytes were almost identical in the characteristics tested.

Chromatography, Ion Exchange↗
PubMed1977

Serum lysozyme activity in some myeloproliferative diseases.

Serum lysozyme activity has been determined in patients suffering from myeloproliferative diseases, chronic myelogenous leukaemia (CML), acute myelogenous leukaemia (AML), chronic lymphatic leukaemia (CLL) and pancytopenia (P). Lysozyme activity was tested in undiluted and tenfold diluted sera. Increased lysozyme activity was found in patients with CML and CLI, whereas there was no change in patients with AML and P. Dilution of sera enhanced lysozyme activity. These data may indicate the presence of inhibitor in the sera tested. The diagnostic significance of the presented findings is discussed.

Humans↗
PubMed1977

The inhibitory effect of heparin on trypsinogen activation with enterokinase.

The effect of heparin on the activation of trypsinogen by enterokinase and on trypsin activity has been investigated. Proteolytic activity of trypsin has been determined using caseinolytic method. It has been found that heparin inhibits trypsinogen activation only when it is preincubated with proenzyme before introduction of enterokinase to the tested system. In these experimental conditions heparin in final concentrations from 1 to 20 U/ml exhibits inhibitory effect diminishing trypsinogen activation to about 65% initial activity and in higher concentrations only to 50%. Heparin introduced to the tested system simultaneously with enterokinase or to the active trypsin did not appear evident inhibitory effect.

Endopeptidases↗
PubMedDate not supplied

[Human serum colony-stimulating factor in mouse bone marrow culture].

Activity of CSF was measured in human serum after chromatography on Sephadex G-200. Agar culture of bone marrow cells was done in Petri dishes with agar in Eagle'a medium containing fetal calf serum and antibiotics. This agar-medium containing mouse bone marrow cells, was allowed to gel and the dishes were incubated at 37 degrees C in a fully humidified atmosphere of 10% CO2 in air. Our data indicate that chromatography procedure allows to get CSF free from its inhibitors. All activity was localized in one peak, in the region of low molecular weight proteins.

Bone Marrow Cells↗
PubMedDate not supplied

[Colony-stimulating factor (CSF) and granulopoietic activity (GA) in various mouse organs].

The colony stimulating factor and granulopoietic activity were determined in the homogenates of mouse kidney, liver, spleen and bone marrow. The activity of CSF and GA were highest in the kidney. The homogenate of this organ was fractionated then by ultracentrifugation. The distribution of CSF and GA in subcellular fractions and homogenates may suggest that kidney cells are the source of a substance causing, both, colony stimulation and granulopoietic activity.

Animals↗