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Biomedical subjects

K Fujiwara

Publications and source records attributed to K Fujiwara.

At least 613 records · Page 34Linked to original sources
PubMed1989

[Contact thermography as a prognostic indicator of breast cancer].

Ninety-seven primary, operable cancer patients were followed after surgery with a median follow-up of 44 months. Thermograms were classified into 3 categories: normal, equivocal and abnormal. There has been no recurrence in patients with normal thermographic pattern. Patients with abnormal thermographic pattern showed poor prognosis: 88% survival and 80% recurrence-free survival rate at 3 years. Normal thermographic pattern favored the survival curves and recurrence-free survival curves, even if stratified by nodal states. There seems to be no correlation between thermographic patterns and sites of recurrence, but patients with abnormal thermographic patterns tend to have recurrences at more than one site.

Breast Neoplasms↗
PubMed1989

[High resolution computed tomography of diffuse interstitial pneumonia during treatment].

HRCT was carried out in twenty patients with diffuse interstitial pneumonia: 13 cases of IIP, 3 of BOOP, 2 of drug-induced pneumonia, 1 of rheumatoid lung and acute interstitial pneumonia of unknown origin. With special attention to inflammatory activity, the patients underwent HRCT periodically during the treatment. Correlative investigation between HRCT image and grade of accumulation in 67Ga scintigraphy was also performed. Response to steroid therapy was clearly reflected on HRCT image, that was shown as decreasing pulmonary density or thinning of honeycomb wall. HRCT is considered to be useful in assessing the activity of diffuse interstitial pneumonia.

Aged↗
PubMed1989

[Follow-up study of precancerous lesions of the breast].

A follow-up study of 105 patients with microdochectomy for nipple discharge during the ten year period from 1965 to 1974 revealed that cancer developed in seven of the cases. The histology of the seven cases consisted of atypical hyperplasia in the terminal duct or lobular neoplasia. Accordingly, patients with either of the above histological findings were regarded as high risks for the development of breast cancer. Twenty such high risk patients were selected from 1850 patients biopsied for benign breast disease during the eleven year period from 1975 to 1985. This follow-up study revealed that five of the 13 patients (38%) with atypical hyperplasia in the terminal duct and two of the seven patients (28%) with lobular neoplasia developed breast cancer. Microphotocytometry using TV image analyzed system showed that the mean +/- standard deviation of the nuclear area and the absorbance were, respectively, 46.79 +/- 2.84 and 0.42 +/- 0.01 in fibroadenoma, 62.93 +/- 12.15 and 0.32 +/- 0.02 in precancerous lesion and 86.16 +/- 14.38 and 0.44 +/- 0.07 in breast cancer.

Breast Neoplasms↗
PubMed1989

Production of Tyzzer's disease in rats by ingestion of bacterial spores.

Tyzzer's disease was produced in rats by peroral inoculation with spores of Bacillus piliformis (Tyzzer's organism) of rat origin. After ingestion of 10(6) spores necrotized lesions with intracellular bacterial propagation were seen in the intestines, liver and heart on days 2 to 14 postinoculation (p.i.). A number of B. piliformis were present within enterocytes of the cecum and colon. Infected cells were also seen in the liver, myocardium and intestinal muscle layers on days 3 to 7 p.i. Infective spores were found to be shed in feces during 3 to 10 days p.i.

Animals↗
PubMed1989

[A case report of compression of right pulmonary artery and bronchus by aneurysmal dilated ascending aorta in tetralogy of Fallot--suspension of ascending aorta].

The patient was a 63-days-old boy who was admitted to our hospital because of moderate cyanosis and tachypnea. After admission, severe respiratory distress and emphysematous change of the right lung on the chest X-ray developed progressively. Echocardiogram and angiocardiogram demonstrated that a tetralogy of Fallot associated with right aortic arch and absence of pulmonary valve, and revealed remarkably dilated ascending aorta which compressed the right pulmonary artery and bronchus. Therefore, the emergency operation in that the ascending aorta was suspended to the 2nd rib was performed through a right thoracotomy. After surgery, his respiratory distress and emphysema of the right lung completely disappeared. To our knowledge, this is the 2nd reported case in which suspension of ascending aorta was successfully performed for pulmonary complication in congenital cardiovascular anomalies as this patient.

Airway Obstruction↗
PubMed1989

[Surgical experiences of Cor triatriatum in infancy].

Three operative cases with Cor triatriatum, age ranged from 30 days to 16 months, were presented. All types were IB1 of Lucas-Schmidt's classification and one patient was associated with right upper PAPVC. One patient died of low output syndrome due to preoperative shock. Communication of abnormal diaphragm in left atrium varied from small and multiple to 5 mm in diameter. Their preoperative diagnoses were established by two-dimensional echocardiogram prior to angiocardiogram and the intracardiac communications were well evaluated by color doppler echocardiogram which was superior to angiocardiogram in this evaluation. Postoperatively, no abnormal diaphragm were detected in two survivors. The diagnosis and operative procedure for this anomaly were discussed on.

Angiocardiography↗
PubMed1989

Enterohepatitis in Mongolian gerbils (Meriones unguiculatus) inoculated perorally with Tyzzer's organism (Bacillus piliformis).

Enterohepatitis was produced in Mongolian gerbils by intragastric inoculation with Tyzzer's organism from natural infection of a gerbil. Death occurred in 50 to 60% animals 5 to 7 days postinoculation (p.i.). On day 3 p.i., when a few necrotic foci appeared in the liver, a large amount of bacterial antigen was present within ileocecal enterocytes and reticuloendothelial cells of the Peyer's patches. Neutrophil and monocyte infiltration was found in the lamina propria. On day 5 or 6 p.i. there was severe necrotizing and hemorrhagic ileotyphlocolitis. Bacterial antigen was abundant within not only enterocytes, but also smooth muscle cells of the ileum and jejunum as well as reticular cells of the mesenteric lymph nodes. On day 7 p.i. the intestinal lesions subsided in the presence of fewer bacteria, while necrotizing hepatitis became well developed. The results indicated that Mongolian gerbils were highly susceptible to the oral route of infection with the Tyzzer's organism.

Animals↗
PubMed1988

Quantitative analysis of the distribution of serotonin-immunoreactive cell bodies in the mouse brain.

The distribution of serotonin neurons in the mouse brain was studied using a peroxidase-antiperoxidase (PAP) immunohistochemical method to obtain quantitative data on serotonin-containing cell bodies. The average total number of perikarya of serotonin neurons was about 26,000, of which about 17,000 cells (66.9%) were situated in the raphe system. The nucleus raphe dorsalis was the main region where a large number of serotonin neurons were present, and it contained about 9000 cells. Other than the raphe system, serotonin-immunoreactive perikarya were seen in the following regions: the reticular formation (18.9%), the lemniscus medialis (8.5%), the nucleus interpeduncularis (1.5%), the subpyramidal region (4.0%) and the periaqueductal gray (0.2%).

Animals↗
PubMed1988

Enzyme-linked immunosorbent assay for the quantification of actinomycin D using beta-D-galactosidase as a label.

An enzyme-linked immunosorbent assay (ELISA) for actinomycin D (AMD) has been developed, which allowed us to measure accurately as little as 50 pg of AMD per assay well. Anti-AMD sera was obtained by immunizing rabbits with an AMD derivative, 7-aminoactinomycin D (7AMD), conjugated with mercaptosuccinyl bovine serum albumin via N-maleoylaminobutyric acid chloride as a coupling agent. An enzyme marker was similarly prepared by coupling 7AMD with beta-D-galactosidase (EC 3.2.1.23) via N-maleoylaminobutyric acid. The ELISA with anti-AMD immunoglobulin G fraction as a solid phase and 7-aminoactino-mycin beta-D-galactosidase conjugate was specific to AMD as well as 7 AMD and showed 30% cross-reaction with actinomycin V, while no cross-reactivity was seen with drugs commonly used with AMD in combination chemotherapy for cancer treatment. The sensitivity of the ELISA was about 1000 times higher than high performance liquid chromatography in detecting AMD in lower concentrations. Using this assay, drug levels were easily measured in the blood and urine of rats following administration of AMD in a single dose of 0.25 mg/kg i.v. These results indicate that the ELISA provides a nonradioactive, inexpensive, sensitive, and rapid method applicable for pharmacological analyses of the drug.

Animals↗
PubMed1988

Sandwich enzyme immunoassay of tumor-associated antigen sialosylated Lewisx using beta-D-galactosidase coupled to a monoclonal antibody of IgM isotype.

Enzyme conjugates with antibody of IgG type have been used extensively in immunohistochemistry, but conjugates with antibody of IgM type have not been reported. This paper describes the beta-D-galactosidase (Gal) labeling of a monoclonal IgM antibody designated CSLEX1 (for cytotoxic sialosylated Lewisx), which is directed against a tumor-associated antigen sialosylated Lewisx (S-Lex). The antibody was first acylated with a heterobifunctional agent N-(gamma-maleimidobutyryloxy)succinimide (GMBS) to introduce the maleimide groups into the molecule; excess reagent was removed by gel filtration and then the activated antibodies were crosslinked to the thiol groups of Gal. The conjugates were partially purified of free Gal by DEAE-Toyopearl column chromatography with an increasing linear concentration of NaCl. The conjugates thus prepared retained almost full enzyme activity and were demonstrated to be free of CSLEX1 by affinity chromatography using anti-galactosidase antibody bound to Sepharose 4B. The conjugates were used as a label in a sandwich enzyme immunoassay (SEIA) to detect the antigen at concentrations as low as 0.2 U/well. The SEIA was used to measure serum S-Lex levels in both healthy subjects and lung cancer patients and mean concentrations of 70 U/ml and 198.6 U/ml were detected respectively.

Animals↗
PubMed1988

The use of N-[beta-(4-diazophenyl)ethyl]maleimide as a coupling agent in the preparation of enzyme-antibody conjugates.

The present study was undertaken to develop a novel method for the enzyme labeling of antibodies. Goat anti-rabbit IgG was used as a prototype and coupled to beta-D-galactosidase (Gal) with a new heterobifunctional cross-linking agent N-[beta-(4-diazophenyl)ethyl]maleimide (DPEM). The antibody was first azo-coupled with DPEM to introduce the maleimide groups into the molecule; excess reagent was removed by gel filtration and then the activated antibodies were crosslinked to the thiol groups of Gal. The conjugates were purified by DEAE-cellulose column chromatography, being completely separated from non-reacted antibodies but remaining mixed with free Gal, and showing approximately 30% enzyme activity bound to antibody. This method is simple, reproducible and so mild that almost full enzyme and antibody activity can be retained. The conjugates were used as a label in an immunoassay and were able to detect first antibody at concentrations as low as 2 ng/tube. Furthermore, the present method was compared with the method using N-(gamma-maleimidobutyryloxy)succinimide (GMBS), and it was found that both conjugates produced comparable results.

Animals↗
PubMed1988

Serotonin reduction in the mouse neostriatum during hyperthermia-induced convulsions studied by immunohistochemistry.

Changes occurring in serotonin neurons during hyperthermia-induced convulsions were examined by means of a modified immunohistochemical method. All mice (8-12 weeks of age) exposed to the temperature of 50 degrees C had convulsions, showing a generalized tonic and/or clonic pattern. Immediately after the convulsions, the animals were perfused transcardially with a fixative. A significant reduction in serotonin immunoreactivity was observed in the neostriatum (caudate-putamen complex) of the mice which had hyperthermia-induced seizures, while the serotonin immunoreactivity remained unchanged in the neocortex and paleostriatum. These results suggest that serotonin may be an important mediator in the mechanism of hyperthermia-induced convulsions or that the susceptibility of serotonin neurons to a convulsive state is greatest in the neostriatum.

Animals↗
PubMed1988

Epidermal growth factor in the mouse kidney: developmental changes and intranephron localizations.

The developmental changes in epidermal growth factor (EGF) have been studied in tissue homogenates, kidney slices, and microdissected nephron segments of the mouse. Immunoreactive EGF concentration per milligram of protein increased in the kidney by about 20-fold from 1 week to 3 weeks of life, reaching the highest levels between 5 and 7 weeks, and decreasing by 10 weeks of life. The time course of the changes was different from those of submaxillary and urinary EGF. Above 7 weeks of age, kidney EGF was higher in female than in male mice. Among various zones of the kidney (outer cortex, inner cortex, outer medulla I, II and papilla), the outer medulla I and II contained the highest quantities of EGF per gram of wet tissue. The highest EGF content per millimeter length was observed in the medullary and cortical thick ascending limbs of Henle's loop. The amounts exceeded by about 4.5-fold those found in glomeruli and in proximal convoluted and proximal straight tubules, and by about 3-fold those present in distal and collecting tubules. Unilateral nephrectomy resulted in no significant changes in EGF levels in the contralateral kidney. The results suggest that the ontogeny of kidney EGF is different from that of the EGF found in the submaxillary gland, and that there is nephron heterogeneity in EGF content.

Animals↗