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Biomedical subjects

L V Beletskaia

Publications and source records attributed to L V Beletskaia.

At least 55 records · Page 3Linked to original sources
PubMed1982

[Bound immunoglobulins and immune complexes in the thymus of myopathy patients].

Profound changes in epithelial and lymphoid tissues were detected by the immunofluorescent technique in the thymus of myopathy patients. The characteristic immunomorphological manifestation indicating injuries to the thymic epithelial tissue is the sedimentation of IgG, IgA and complement in basal membranes and in the cytoplasm of adjacent poorly differentiated epithelial cells. Another remarkable sign of the changes in the thymic epithelial tissue during the disease is a sharply marked cystic degeneration of Hassall bodies, eventuating in the sedimentation of keratinized material in the internal environment of the organ. The changes in the thymic lymphoid tissue during myopathy are confirmed by the sedimentation of immune complex granules, containing IgM, IgA, IgG, and complement, in the intercellular space bordering individual groups of corticomedullar zone lymphocytes. The data obtained suggest that the thymic changes play an important part in the development and course of progressing muscle dystrophy.

Adolescent↗
PubMed1982

[Basal cell cross-reacting antigen in normal and neoplastic human tissues].

The method of immunofluorescence was used to study location of the basal cell cross-reacting antigen shared by group A streptococcus, and multilayer epithelium of the mucous membrane of the oral cavity, oesophagus, vagina, cervix, urinary bladder, eye conjunctiva, epithelial cells of the mammary and salivary gland ducts of man. The cross-reacting antigen possessing tissue specificity was found in human tumor cells histogenetically associated with the tissues carrying the antigen (basal cell carcinoma, squamous cell carcinoma of the skin, larynx, cervix) but not in the cells of tumors of different genesis (infiltrative mammary gland carcinoma, adenocarcinomas of the stomach and intestines). The results obtained will serve as the basis for the development of an immunological method for differential diagnosis of poorly-differentiated human tumors and for some problems of histogenesis of neoplasias.

Adenocarcinoma↗
PubMed1982

[Myoid cells in the thymus of patients with myopathy].

Immunofluorescence was used to demonstrate that in progressive muscular dystrophy, myoid cells in the patients' thymus undergo profound changes which affect the processes of their formation, differentiation and maturation. Pronounced changes in the myoid cells were disclosed in two forms of myopathy. These changes lie in the increased number of myoid antigens in Erb's myopathy, whereas in Duchenne's myopathy, they manifest in the diminution of the number of myoid antigens in the internal environment of the thymus. It is suggested that the differences in the pattern of changes in the myoid cells, apart from other factors, apparently govern the peculiarity of the pathological process in the two forms of muscular dystrophy.

Adolescent↗
PubMed1982

[Characteristics of the reaction of healthy human blood serum with the epidermis].

Indirect immunofluorescence has shown that the blood serum of normal subjects reacts with cytoplasm antigens of epidermis differentiated cells in 100% of the cases. The level of antibodies and the immunomorphological picture of the reaction are marked by high constancy and intense fluorescence of the cytoplasm of epidermis differentiated cells, while the reaction with basal layer cell antigens is observed comparatively seldom and little pronounced. The authors discuss possible participation of antibodies contained by the normal blood serum and of their complexes with tissue antigens in the regulation of vital activity of the cells and immune response to the host antigens.

Adult↗
PubMed1981

[Cross-reacting antigens of human thymus myoid cells and stable L-forms of group A streptococci].

Indirect immunofluorescence has shown a similarity between the antigen components of group A streptococcus L-forms and human thymus myoid cells. An analogous antigen (or antigens) is present in the cytoplasmic membrane of human myocardial cell fibers. The depletion of antiserum to the streptococcal L-forms both by the culture of L-forms grown in meat or casein media and by the homogenate of the cardiac muscle leads to the inhibition of immunofluorescence. The depletion of serum by the homogenate of other tissues (liver) or by L-form culture does not virtually affect the immunofluorescence intensity. According to the authors' opinion, the similarity of antigens of group A streptococcus L-forms to the antigenic components of organ tissues is likely to be responsible for long-term persistence of the microorganisms under consideration and to favour, in some cases, the occurrence of autoantibodies. The latter circumstance might lead to pathological changes in organs containing cross-reacting antigens.

Antigens↗
PubMed1981

[Detection of a cross-reacting antigen common to multilaminar epithelia and group A streptococcus in cells of human epidermal tumors].

The tissue-specific antigen of basal cells of human squamous epithelium which cross-reacts with streptococcal group A antigen has been detected in the cytoplasm of cells of human basal cell and squamous cells carcinomas histogenetically related to the skin epithelium. The antigen has not been detected in cells of tumors of endodermal origin (gastric and intestinal adenocarcinomas). These findings together with previous reports on the existence of cross-reactivity in case of animal tumors of ectodermal genesis may form the basis for an additional method of differential diagnosis of human tumors arising from the ectoderm-derived epithelium.

Antigens↗
PubMed1981

[Collector system of the thymus].

A method of silver impregnation of the microcirculatory bed was used to disclose in thick sections (30-80 micrometers) of the guinea-pig thymus cortical zone a vascular network consisting of arteriolo-capillaro-venular loops resembling those seen in the derma papillary layer. The arteriolar and venular loops belong to the cortical zone, whereas the capillary loops lie in the corticomedullary zone. Since the cortical layer demonstrates a local distinctly marked histohematic barrier, poorly accessible for lymphocytes with the capillary loop wall being readily permeable, the lymphoid cells, before egress from the organ, travel across the entire cortical layer where they undergo influences of numerous differentiation factors over a certain period of time. It is assumed that the microcirculatory network disclosed is the basis for the thymus collector system responsible for egress of mature T cells from the organ.

Animals↗
PubMed1981

[Immunofluorescence study of changes in myoid cells in the thymus of myasthenia patients].

It has been established that the mean number of myoid cells in one visual field in the thymus from myasthenia patients exceeds normal approximately twofold, with significant individual fluctuations being recorded. The observation data allow one to distinguish two basic forms of changes in myoid cells in myasthenia: (1) virtually complete absence of myoid cells leading to deficiency of myoid antigens; (2) more frequently seen hypertrophy and the increased number of myoid cells responsible for excess myoid antigens in the thymus. Basing on the fact that hypertrophy of myoid elements is seen, in most cases, in the presence of a pronounced hypoplasia of the lymphoid tissue, a suggestion is made about derangement in myasthenia of a mechanism by which the ratio of lymphoid and myoid cells in the thymus is controlled.

Adolescent↗
PubMed1981

[Cross-reacting antigen common to stratified squamous epithelium and Streptococcus group A in tumors of epidermal origin].

A tissue-specific basal-cell antigen of stratified squamous epithelium which enters into cross reaction with group A streptococcus polysaccharide was detected in the cytoplasm of human basal- and squamous-cell tumor cells which are associated histogenetically with skin epithelium. However, it was not found in the cells of entodermal tumors (stomach and intestinal adenocarcinoma). Considering earlier reports on the detection of cross reacting antigen in experimental ectodermal tumors, these results suggest the development of an additional histogenetical method of differential diagnosis of human tumors which arise in ectodermal tegumental epithelium.

Adenocarcinoma↗
PubMed1981

[Use of whole loose connective tissue preparations in immunofluorescence studies].

Evidence has been obtained for potential use of total loose connective tissue film preparations during immunofluorescence studies of antinuclear antibodies of serum from patients with systemic lupus erythematosus, antibodies to cytoplasmic components of fibroblasts of serum from patients with rheumatoid arthritis, antibodies to the surface fibroblast antigen fibronectin, and of certain regularities in IgG binding with mast cell components. As compared with other preparations (cryostat, paraffin sections) used in immunomorphology while applying the methods of labeled antibodies, the total preparations have the following basic advantages: simplicity of preparation and possibility to eliminate the denaturative effects on tissue structures.

Animals↗
PubMed1981

[Venous immunomorphology in acute experimental thrombosis and human recurrent migrating thrombophlebitis].

The direct immunofluorescence procedure was used to demonstrate immune complexes (fixed immunoglobulins) in the internal and external sheath of veins of a dog with experimental acute thrombosis caused by partial ligation of the ileofemoral vein in the presence of immunization of the animal with extracts of auto- and homologous vein tissue. The results suggest the participation of auto-immune processes in the pathogenesis of acute thrombosis of the main veins.

Acute Disease↗
PubMed1980

[Immunofluorescence study of reactions between the serum of patients with rheumatoid arthritis and components of connective tissue cellular elements].

By means of immunofluorescence using sera against three classes of human immunoglobulins (IgG, IgM, IgA), localization of the components (or one of the components) responsible for reaction of sera from patients with rheumatoid arthritis (RA) with connective tissue antigens was found to be in the cytoplasm of different kinds of cell elements of the connective tissue present in joints. Sera from RA patients collected at different stages of the disease reacted with connective tissue cell elements in titers of 1 : 64 to 1 : 1024, whereas sera from normal subjects (in 33% of specimens) reacted only to 1 : 8--1 : 16.

Animals↗
PubMed1980

[Cross-reacting antigen common to cells of the basal layer of monolayer epithelium and the polysaccharide of group A streptococci during embryogenesis].

Indirect immunofluorescence with the use of antibodies to the cross-reactive antigen (CRA) common to the group A streptococcus as well as to human and animal cover epithelia of ectodermal origin has shown that CRA appears in the Wistar rat fetal skin on the 15th day of pregnancy in the early period of stratified squamous epithelium formation. The antigen is diffusely spreading in the cytoplasm of large cells of the embryonal skin epithelium. By the end of pregnancy and at the moment of birth (days 21--23) the pattern of CRA localization in fetal skin is the same as the skin of adult species: it is localized only in the peripheral zone of basal layer cell cytoplasm of the stratified squamous epithelium.

Animals↗