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Biomedical subjects

L V Beletskaia

Publications and source records attributed to L V Beletskaia.

At least 73 records · Page 4Linked to original sources
PubMed1980

[Morphological studies in delayed hypersensitivity to streptococcal antigens using film preparations of the mesentery].

Film preparations of the mesentery were used for morphological study of cell reactions, connected with the delayed type hypersensitivity (DTH). Twenty-four hours after intraperitoneal injection of small doses of the streptococcal thermostable fraction (TST fraction) the guinea-pigs sensitized with streptococcal culture developed a more intensive, as compared with control animals, diffuse infiltration of the peritoneum with mixed type cells (lymphocytes, monocytes and polynuclear leukocytes) abundant in mononuclear elements. With TST fraction dose build up the infiltration demonstrated an abrupt increase in polynuclear leukocytes completely masking the monocytic reaction in some cases. Large aggregations of lymphocytes were seen in the prelymph sinuses accompanying blood capillaries and nervous stemlets. Detection in the peritoneum of cell reactions characteristic of DTH following the injection of certain doses of specific antigens and comparative simplicity of obtaining film preparations allow recommending the technique described for disclosing and experimental study of DTH to microbial antigens.

Animals↗
PubMed1980

[Reaction between myasthenia patients' sera and the antigenic structure of the surface of thymic lymphocytes of healthy subjects and myasthenia gravis patients].

It has been established that sera from myasthenia patients contain in 42% of cases IgG antibodies to the point antigenic structure on the lymphocyte surface of the human thymus, spleen and lymph nodes. This antigenic structure is less pronounced on the lymphocytes from the thymus of normal subjects as compared with lymphocytes from the spleen and lymph nodes. On the contrary, the thymocytes from myasthenia patients show an elevated content of the antigen in question and do not differ in this respect from lymphocytes of the peripheral lymphoid organs of normal man.

Adolescent↗
PubMed1980

[Distribution of a cross-reacting antigen common to group A streptococcal polysaccharide and multilayer squamous epithelium in different human organs].

Indirect immunofluorescence with the use of antibodies) to group A streptococcal polysaccharide (A-polysaccharide has shown cross-reacting antigen (CRA) common for A-polysaccharide and the squamous epithelium to be localized, apart from the skin and thymus, in basal layer cells of oral mucosa epithelium, esophagus, inferior portion of the rectum, in salivary and mammary ducts. These tissue carriers of CRA are united by common ectodermal origin. The evidence obtained may be of use in the devising of a method for differential diagnosis of tumors of different genesis. In the course of organogenesis CRA appears early in the monolayer embryonic epithelium. The data presented correlate with those obtained before during study of animal tissues.

Adolescent↗
PubMed1980

[Immunofluorescence study of lactoferrin-synthesizing thymus cells].

Lactoferrin-synthesizing cells were detected in the human thymus by immunofluorescence. LF cells in the thymus were shown to be more numerous, to possess greater secretory activity, and to appear earlier in the process of embryonal development as compared to the organ producing this protein (salivary gland). The data obtained suggest that in the thymus, lactoferrin apart from its known functions, bacteriostatic and ferrous iron-binding ones, acts as a heteroorgan antigen, i.e. provides lymphocytes of the thymus with information on this protein structure during natural immunologic tolerance induction.

Adolescent↗
PubMed1980

[Study of the argyrophilic structures of thymus connective tissue following exposure of the animal to x-rays].

It was shown that the argyrophil structures of animal thymus connective tissues (reticulum fibers, reticulum cells, histocytes) are resistant to x-rays, preserving their structure and argyrophil properties when exposed to large doses (1000-3000 rad). In irradiated animals the structures enumerated being demasked because of emigration and destruction of lymphocytes are identified more adequately than those in intact animals. In the cortical zone, the reticulin fibers were largely contained by vascular walls. In the medullar zone, the thin fibers formed the network reaching the Hassal bodies. Argyrophil reticular cells and histocytes were spread through the whole lobule, the largest groups of histocytes being located around the Hassal bodies.

Animals↗
PubMed1978

[Bound class M, A and G immunoglobulins in the thymus of myasthenia gravis patients].

Deposits of granular material containing IgM, IgA, and IgG were revealed in the thymus of patients with myasthenia by direct immunofluorescence. Treatment of the thymus sections by unlabeled preparations against individual classes of human immunoglobulins inhibited the reaction of the granular material with the homologous labeled preparations. Disappearance of fluorescence of these deposits was also seen in treatment of the sections with glycine-HCl-buffer, pH 2.8. These data permitted a suggestion that granular material represented immune complexes where IgM, IgA, and IgG served as antibody, and thymus tissue components--as an antigen. The presence of bound immunoglobulin in the thymus indicated that an autoimmune process directed against the tissues of this organ occurred in myasthenia.

Autoantibodies↗
PubMed1978

[Common antigens of stable group A streptococcal L forms and muscle fibers of human myocardium].

The immunofluorescence method was used to demonstrate that the antigens of stable streptococcus L-forms and of the cytoplasmic membrane of human myocardium muscle fibers were common. The common antigen was included into the composition of the surface membrane of the muscle cell adjacent to the sarcolemma, and of the transverse tubule membranes of the sarcoplasma reticulum passing in the Z-band region of the muscle fiber sarcomeres. The reactions is completely prevented by the exhaustion of the anti-serum to the antigen of the L-forms with the homogenate of human myocardium tissues or a suspension of the L-form cultures grown both on meat and on casein media. Exhaustion with tissue homogenate of other organs (the liver) or with concentrated nutrient medium practically failed to influence the extent of this reaction. The authors believe that the commonnes of the antigens of stable L-forms of streptococcus, group A, cultures and of the myocardium could serve as one of the causes of prolonged persistence of L-forms in human and animal organism.

Animals↗
PubMed1978

[Comparative study of the myoid cells of human embryonal and adult thymus by the indirect immunofluorescence method].

The indirect immunofluorescence method showed that decreased (in comparison with myoid cells of adult human thymus) content of muscle antigens in the myoid elements of the embryonic organ caused a greater secretory activity of these elements at the early embryogenesis. Due to increased secretory activity of the myoid cells internal medium of the embryonic thymus contained more antigens common to the muscle tissue than the adult human thymus. The fact that during the ontogenesis the functional activity of the myoid cells correlated with the rate of lymphoid tissue formation favours a suggestion that heteroorganic antigens provide the thymus lymphocytes with information concerning the autoantigen structure necessary to induce natural immunological tolerance.

Adult↗
PubMed1977

[Antibodies to an antigen of human thymus epithelial tissue common to the epidermis of the skin in malignant myasthenia].

It has been demonstrated by the indirect immunofluorecent technique that many of the sera of patients with myasthenia gravis react with the anticells of the human thymus epithelial tissue. Sorption of the sera with the suspension of the epidermis cells and the homogenates of the tissues of other human organs showed that the epithelial cell antigen with which the sera of patients with myasthenia reacted were epidermal heteroorganic thymus antigens, i.e. common for the thymus epithelium and skin epidermis. The presence of antibodies to the cells of the epithelial tissue of the thymus in the sera of patients suffering from myasthenia gravis permits to suppose the existence of an immunopathological process against the thymus tissue antigens (including the heteroorganic structures of its epithelium) in this disease.

Antibodies↗
PubMed1977

[Study of the myoid cells of the thymus by the immunofluorescent method].

Muscular proteins were found in the cytoplasm of myoid cells of the thymus in all investigated species (chicken, mouse, rat, guinea pig, rabbit, cattle, human) by the method of immunofluorescence using the human serum containing antibodies to antigens of muscular fibres of striated muscles and fluorescent antibodies to human immunoglobulins. Under study were the characteristic features of the content and localization of the myoid antigen in the sarcolytes of the thymus of animals and man. The obtained data show a permanent presence of muscular antigens in the internal medium of the thymus and speak in favour of their possible participation, in addition to other heteroorganic antigens of the thymus, in the formation of natural immunological tolerance to the proper antigens of the organism in the process of differentiation of thymocytes.

Animals↗
PubMed1977

[Permeability of cutaneous macrophage lysosomal membrane in delayed hypersensitivity to streptococcal antigens].

The acid phosphatase (APh) was studied in the skin macrophage lysosomes of animals with delayed-type hypersensitivity (DTH) to the antigens of group A streptococci or tuberculoproteins. The histochemical procedure for the APh determination was employed in the skin sections without any preliminary tissue fixation. Intradermal injection of specific antigens in DTH proved to lead to the increase of the lysosomal membrane permeability in the skin macrophages. These data support the supposition that the lysosome enzymes of macrophages can act as factors causing tissue destruction in DTH.

Acid Phosphatase↗
PubMed1977

[Cross-reacting epithelial antigen of ectodermal origin in mouse tumors].

Cross-reacting antigen (CR-antigen) previously found by indirect immunofluorescence in cell elements of murine multilayer epithelial cells (except corneal epithelium) to play the role of antigenic label of cover epithelia of ectodermal origin was found in mice tumor cells, related histogenetically with multilayer epithelium (transplantable proventricular cancer, two strains of squamous-cell cervical cancer in mice). CR-antigen was observed in transplantable breast adenocarcinoma and in transplantable mice teratocarcinoma.

Adenocarcinoma↗
PubMed1976

[Autoantibodies to the common antigenic determinant of group A streptococcal polysaccharide and epithelial cells of mammalian thymus and skin].

A cross-reactive (CR) antigenic determinant in the polysaccharide of group A streptococus and the mammalian thymus and skin was studied. The CR antigen was found in adult humans and human embryos irrespective of the blood group, as well as in the tissues of all the animal species studied, including rabbits immunized with streptococcus and producing antibodies to A polysaccharide. Evidence was obtained that the antibodies elaborated to the CR determinant of polysaccharide A and of the tissue epithelium should be classed as autoantibodies. It is suggested that reaction of these antobodies with the CR antigen in the thymus could serve as one of the causes of autoimmune thymitis during rheumatic fever.

Adult↗
PubMed1976

[Distribution of a cross-reacting antigen common to epithelium and group A streptococci in human and animal tissues].

With the aid of pure antibodies to polysaccharide of streptococcus, of group A, there was revealed by the method of indirect immunofluorescence with the use of pure antibodies to rabbit immunoglobulins (labeled with fluoresceine isothiocianate) localization of the cross-reacting antigen common for streptococcus and tissues of man and animals in the cells of surface epithelium of the ectodermal origin (the skin, salivary ducts, oral mucosa, esophagus, inferior portion of the rectum, uterine cervix, and the elements of the thymus epithelium). Detection of the cross-reacting antigen was of interest in connection with the study of the mechanisms of development of chronic pathological processes in man associated with streptococcus infection and investigation of the structure, function and pathology of the thymus.

Animals↗