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Biomedical subjects

M M Gapparov

Publications and source records attributed to M M Gapparov.

At least 55 records · Page 3Linked to original sources
PubMed1989

[Effects of sodium oxybutyrate on the level of catecholamines and serotonin and monoamine oxidase activity in patients with alcoholism].

Sodium salt of gamma-hydroxybutyric acid (Na-GHB) was administered to 37 drug-free alcoholic men in placebo controlled study. The psychotropic effect was evaluated using psychometric scale of 4 ranges. Changes in plasma levels of epinephrine, norepinephrine, dopamine, serotonin and platelet MAO-B activity were assessed. The psychotropic effect was observed in 27 (76%) patients. The baseline of dopamine concentration was significantly correlated with the expression of psychotropic effect. Na-GHB decreased dopamine concentration in responders. The possible involvement of dopaminergic system in the realization of psychotropic effect of Na-GHB was discussed.

Adult↗
PubMed1985

[Turnover of the amino sugars of erythrocyte and thrombocyte glycoproteins in rats maintained on different carbohydrate diets].

The turnover of erythrocyte and platelet glycoprotein amino sugars in rats fed carbohydrates has been studied. Partial replacement of starch by sucrose results in an almost 3-fold increase in the half-life of erythrocyte glycoprotein amino sugars and in a 2-fold increase in that of platelets as early as 14 days after keeping the animals on carbohydrates. It is concluded that study of dynamic parameters of cell amino sugars can be used for evaluation of the role played by food in metabolic and adaptive reactions both in experimental animals and man.

Amino Sugars↗
PubMed1984

[Effect of thyroid state on the phospholipid regeneration rate of rat liver mitochondria].

The rate of phospholipid renewal in mitochondria of normal, hypothyroid, hyperthyroid and thyrotoxic rats was studied. Mitochondria were isolated from rat livers 24 and 48 hrs after administration of 3H-glycerol and 14C-palmitic acid. In mitochondria of hypothyroid animals, practically no phospholipid renewal is observed. In mitochondria of hyperthyroid and thyrotoxic rats, the rate of degradation of phospholipids labelled with glycerol, was approximately 1.5 times as low as that in controls. The decrease in the rate of renewal of the fatty acid residues in mitochondrial phospholipids was still more pronounced in hyperthyroid and thyrotoxic animals. Possible reasons for these changes connected with the thyroid state are discussed.

Animals↗
PubMed1982

[Effect of protein on the induction and renewal of a cytochrome P-450 complex in the rat liver].

It was disclosed that the effect on the content of cytochrome P450 and renewal of the protein portion of the cytochrome P450 complex in rat liver microsomes is determined by the protein amount in the diet. Protein deficiency in the diet leads to the reduced rate of protein fraction renewal in the cytochrome P450 complex. The degree of cytochrome P450 induction with phenobarbital was found to be dependent on the diet. In the animals fed low-protein diet (4.5%), the degree of cytochrome P450 induction was decreased which was likely to be a consequence of the increased semi-renewal period.

Animals↗
PubMed1982

[Protein turnover of subcellular rat liver fractions in a varying protein supply of the body].

Experiments on rats were made to study turnover and the content of total proteins of rat liver subcellular fractions under isocaloric diets with different amounts of protein. As a result of receiving low-protein diets (9 and particularly 4.5% as regards protein), the half-life of 14 C-lysine decreases apart from a certain reduction in the protein content in all the fractions. Upon unchanged protein content in respect of the control (18% protein), high-protein diet also leads to the half-life diminution. An increase in protein turnover by 4.5% as regards protein in the diet correlates with overall and specific activities of cytochromooxidase in the inner membranes of mitochondria.

Animals↗
PubMed1980

[Metabolism of lipid-carrying proteins of rat liver in normal rats and after phenobarbital injection].

A protein fraction possessing a 209 times higher lipid-carrying activity in comparison with the original supernatant (X 105 000 g) was isolated from the livers of Wistar rats. The purification procedure included precipitation at pH 5,1, desalting with ammonium sulfate and gel-filtration through Sephadex G-50. Evidence from polyacrylamide gel electrophoresis suggest that the fraction obtained consists of two components with molecular weights of 18 000 and 30 000. The half-life time of the proteins as determined by double radioactive labelling is 3,7 days. The microsomal oxidation inducer phenobarbital increases the half-life time of lipid-carrying proteins by decreasing their degradation rate.

Animals↗
PubMed1976

[A method of studying hydrochloric acid secretion in isolated gastric mucous membrane using a two-channel pH-meter].

A new method of hydrochloric acid secretion recording on two symmetrical parts of the same isolated gastric mucosa is described. A two-channel system which had a number of important advantages over a single-channel system was used. The advantage of this method lay in a possibility of a more exact differentiation of the influence of different substances on the rate of H+ secretion and in a considerable decrease in the number of animals required for the experiments. The apparatus scheme for a two-channel recording of H+ secretion, and design of the four-chamber cuvette are described.

Gastric Acidity Determination↗
PubMed1975

[Morphologic, biochemical and functional properties of the mitochondria of the rabbit gastric mucosa].

Purified fractions of the rabbit gastric mucosa and liver mitochondria were isolated. Electrone microscopy and functional studies of mitochondria showed the most intact mitochondria of gastric mucosa to be localized in gradient fractions of p=1.16-1.18, and those of the liver in fractions with p=1.19-1.20. The gastric mitochondria differed from the liver mitochondria by specific localization in oxyntic cells, abundance of crists, small matrix volume, presence of high respiratory control at lesser values of pH, high respiratory activity, great amount of cytochromes of electron transport chain in inner mitochondria membrane, two-fold excess of cytochroma a in relation to b and c, absence of b5 and by presence of great amount of phosphathydilaethanolamine and unsaturated fatty acids.

Acid Phosphatase↗
PubMedDate not supplied

[Non-enzymatic glycosylation of dietary proteins by glucose and fructose].

Preliminary evaluation of glucose and fructose as glycosylation agents of some animal and vegetable food proteins has been presented. The study was conducted using the method of radioactive indicators. It has been shown that fructose binds proteins to a higher degree than glucose, and it can make for increasing glucose glycosylating activity in their combination in the product.

Dietary Proteins↗