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Biomedical subjects

M Mukherjee

Publications and source records attributed to M Mukherjee.

At least 109 records · Page 6Linked to original sources
PubMed1983

Studies on TI-2 trypsin inhibitor of Streptomyces griseus.

The trypsin inhibitor TI-2 produced by Streptomyces griseus Cal in a medium, containing beef extract and peptone as the nitrogen source, was purified by ammonium sulfate precipitation and gel filtration. Amino acid analysis showed that it contained hydroxyproline and carbohydrate whereas tyrosine, tryptophan, and cystine were absent. The composition of the inhibitor protein thus showed a similarity to collagen. Growth of the organism in a medium with gelatin as the sole nitrogen source resulted in the production of the same TI-2 inhibitor. Incubation of gelatin with either concentrated culture filtrate, trypsin, or chymotrypsin also yielded trypsin inhibitors. The product isolated from the in vitro incubation of gelatin with trypsin appeared to be the same as TI-2. To the author's knowledge, this is the first report that peptides obtained by enzymic hydrolyses of gelatin are trypsin inhibitors.

Animals↗
PubMed1982

Effect of operation on peripheral lymphocyte counts and production of adenosine triphosphate (ATP) in cancer patients.

Lymphocyte bioenergetics in cancer patients undergoing surgery is largely unexplored. The effect of surgery on total lymphocyte counts and adenosine triphosphate (ATP) production is reported. Lymphocyte ATP was assayed by the coupled enzymatic method using hexokinase and glucose 6-phosphate dehydrogenase and lymphocyte counts were calculated from total leukocyte counts and differential counts. Lymphocyte ATP assay and counts were carried out in 13 patients pre- and postoperatively, and lymphocyte counts were scored in six more patients similarly. In 69% of the patients lymphocyte ATP production was depressed postoperatively, and this condition appeared to be associated with cancer spread and immunosuppressive tumors. Postoperative increase in lymphocyte ATP was observed following hysterectomy, colostomy, and also after blood transfusion. The significance of the findings are discussed. Changes in postoperative lymphocyte counts correlated with lymphocyte ATP in 62% of the cases.

Adenosine Triphosphate↗
PubMed1982

Trypsin inhibitor of Streptomyces griseus grown in a carbon nutrition medium.

Streptomyces griseus grown in beef extractpeptone medium produces trypsin inhibitors which are both peptide and protein in nature. When the strain was grown in carbon nutrition medium, only one trypsin inhibitor was obtained. Similar elution patterns of this inhibitor and the TI-3a inhibitor obtained from the beef extract-peptone medium on Sephadex G-15 and ion-exchange columns and also the RF values indicate that the inhibitors from both the sources are the same. So, TI-3a is not the breakdown product of the protein inhibitor of S. griseus.

Culture Media↗
PubMed1975

Erythropoiesis and erythropoietin in hypo- and hyperthyroidism.

Qualitative and quantitative studies of erythropoiesis in 23 patients with hypothyroidism and 21 patients with hyperthryoidism included routine hematologic evaluation, bone marrow morphology, status of serum iron, B12 and folate red blood cell mass and plasma volume by radioisotope methods, erythrokinetics and radiobioassay of plasma erythropoietin. A majority of patients with the hypothyroid state had significant reduction in red blood cell mas per kg of body weight. The presence of anemia in many of these patients was not evident from hemoglobin and hematocrit values due to concomitant reduction of plasma volume. The erythrokinetic data in hypothyroid patients provided evidence of significant decline of the erythropoietic activity of the bone marrow. Erythroid cells in the marrow were depleted and also showed reduced proliferative activity as indicated by lower 3H-thymidine labeling index. Plasma erythropoietin levels were reduced, often being immeasurable by the polycythemic mouse bioassay technique. These changes in erythropoiesis in the hypothyroid state appear to be a part of physiological adjustment to the reduced oxygen requirement of the tissues due to diminished basal metabolic rate. Similar investigations revealed mild erythrocytosis in a significant proportion of patients with hyperthyroidism. Failure of erythrocytosis to occur in other patients of this group was associated with impaired erythropoiesis due to a deficiency of hemopoietic nutrients such as iron, vitamin B12 and folate. The mean plasma erythropoietin level of these patients was significantly elevated; in 4 patients the levels were in the upper normal range whereas in the rest, the values were above the normal range. The bone marrow showed erythyroid hyperplasia in all patients with hyperthyroidism. The mean 3H-thymidine labeling index of the erythroblasts was also significantly higher than normal in hyperthyroidism; in 8 patients the index was within the normal range whereas in the remaining 13 it was above the normal range. Erythrokinetic studies also provided evidences of increased erythropoietic activity in the bone marrow. It is postulated that thyroid hormones stimulate erythropoiesis, sometimes leading to erythrocytosis provided there is no deficiency of hemopoietic nutrients. Stimulation of erythropoiesis by thryoid hormones appears to be mediated through erythropoietin.

Biological Assay↗
PubMed1973

Primary biliary cirrhosis in India.

In a prospective search to study the occurrence of primary biliary cirrhosis, only one patient with this disease was encountered during the last six years. This is also the first documented case of primary biliary cirrhosis from the Indian subcontinent.

Bile Ducts↗
PubMedDate not supplied

Study of 1,26,266 consecutive births for major congenital defects.

Retrospective study was carried out in 1,15,851 consecutive births in five hospitals, and prospective study of 10,415 consecutive births in one hospital of West Bengal. The overall frequency of congenital defects was 4.42/1000 live births. Incidence of NTD and talipes was predominant in rainy season, whereas cleft palate with/without hare lip had highest frequency during winter. Incidence of these anomalies decreased with increasing birth order. The frequency was noted to decline with increasing maternal age, 21-25 years age group had maximum number of malformed babies. Sex ratio and religion, however, did not have any correlation with the incidence of these malformations. When comparison was done between urban and rural population no statistically significant variation was observed.

Congenital Abnormalities↗