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Biomedical subjects

M Ogino

Publications and source records attributed to M Ogino.

At least 127 records · Page 7Linked to original sources
PubMed1985

Productivity of estrogens by human placental organ culture at different stages of gestation.

The productivity of estrogens from 6 to 39 weeks of pregnancy was assessed by using human placental organ culture. It was revealed that the placenta gains the capacity to generate a significant amount of estrogens at around 8 weeks of pregnancy. Therefore, it was suggested that the luteoplacental shift takes place between 6 and 8 weeks of gestation (or 4 to 6 weeks after fertilization). The productivity of estrogens showed a distinct difference before and after 10 weeks of pregnancy when the placental localization is established. The amount of estrone formed by the placenta before 10 weeks of pregnancy was 0.043 +/- 0.005 nanomoles/mg protein/hour (mean +/- s.e, n = 6), while it was 0.034 +/- 0.002 nanomoles/mg protein/hour from 10 weeks to term (mean +/- s.e, n = 18 ; not significantly different) during which no marked change was observed, however that of 17 beta-estradiol was 0.099 +/- 0.002 nanomoles/mg protein/hour (mean +/- s.e, n = 6) before 10 weeks of pregnancy and 0.072 +/- 0.002 nanomoles/mg protein/hour (mean +/- s.e, n = 18) from 10 weeks to term (significant difference with p less than 0.005). The 17 beta-estradiol/estrone ratio remained almost constant throughout pregnancy, which ranged from 2.00 to 3.00.

Androstenedione↗
PubMed1985

[Study on the histopathological abnormalities of the umbilical cord].

There have been very few reports of studies on histological abnormalities of the umbilical cord. In the present study, we have investigated various abnormalities of the cord with special emphasis on the cord vasculature and the vestigial remnants based on developmental abberations. The incidence of single umbilical artery was 1/509 (0.2%), by which no special relation was signified between the single artery and the occurrence of congenital malformations of the neonate. Supernumerary umbilical vessels were found in 5/509 (1.0%) of the cases examined. Three out of five revealed the umbilical cord with two arteries and two veins, while two of them showed the umbilical cord with the normal 3 vessels associated with an accessory fourth vessel. It is of a great interest to note that fetal distress with or without fetal acidosis was demonstrated in all cases with the accessory fourth vessel, whereas the presence of supernumrary umbilical vessels was least related to the occurrence of congenital malformations of the neonates. The incidence of vestigial remnants was 30/509 (5.9%). The vestigial remnants were classified into two groups according to their epithelial pattern, i.e. flat epithelium and cuboidal or columnar epithelium. The vestigial remnants with flat epithelium type, considered to originate in the allantoic duct, were found in 2/3 (66.7%) of all cases with vestigial remnants. The vestigial remnants with cuboidal or columnar epithelium, considered to originate in the omphalomesenteric duct, were found in 1/3 (33.3%) of all cases with vestigial remnants, one of which case was associated with congenital malformation of omphalocele of the neonate.

Arteriovenous Malformations↗
PubMed1984

Difference of bond bonding behavior among surface active glasses and sintered apatite.

Three types of surface active glasses and sintered apatite were implanted in femurs of rabbits for 8 and 16 weeks and subjected to the push-out test to measure the bone bonding strength. The apatite surface layers of these materials, which were formed under in vitro treatment, were studied using IR, NMR, and AES. The difference in the bonding strength among these materials was shown statistically. The results of in vitro experiments explained the differences. It was concluded that the crystal chemistry and formation rate of the surface apatite layer significantly influenced the bonding strength. Materials that formed a surface apatite layer having a bone-like crystal chemistry and fast formation rate showed high bone bonding strength.

Animals↗
PubMed1984

Viability of the human placental villi in organ culture.

The viability of the placental villi in organ culture was investigated both from the morphological and functional points of view. Morphological viability was judged on the appearance of the villi with special emphasis on the trophoblastic cells and the morphological integrity of the placental villi could be maintained for at least 96 h in organ culture. Functional viability was judged by analyses of delta 5-3 beta-hydroxysteroid dehydrogenase; delta 5,4-isomerase (3 beta-HSD) activity with 14C-pregnenolone as the substrate and by the suppressive effect of the cultivated villi on the secretion of plasminogen activator (PA) obtained from mouse peritoneal macrophages stimulated by OK-432. It was found that the 3 beta-HSD activity and suppressive effect on PA secretion could be preserved for at least 120 h in organ culture.

3-Hydroxysteroid Dehydrogenases↗
PubMed1984

[Leukocyte migration inhibitory factor of synovial fluid lymphocytes in rheumatoid arthritis].

Synovial fluid lymphocytes from patients with rheumatoid arthritis were examined for the presence of leukocyte migration inhibitory factor by the agarose technique of Clausen. T and B lymphocytes were stimulated with phytohemagglutinin, concanavalin A, and pokeweed mitogen, and the culture supernatants were used for the migration inhibitory test. Migration index of the culture supernatants of T cells from rheumatoid synovial fluids was significantly greater than those of peripheral blood from rheumatoid patients and healthy controls. The results indicated that the production of leukocyte migration inhibitory factor following stimulation by mitogens was decreased in the T cells of rheumatoid synovial fluid. In the rheumatoid synovial fluid there was a positive correlation between the migration index and polymorphonuclear leukocyte counts. A similar result was also obtained in the study of B cells from rheumatoid synovial fluid. These results suggest that the rheumatoid synovial lymphocytes have been already activated in the synovial fluid. On the other hand, the supernatants of rheumatoid synovial fluid showed an elevated migration index, exceeded 1.0 and higher than those of osteoarthritic synovial fluid. This suggests that chemotactic factors play an important part in the rheumatoid synovial fluid, though the leukocyte migration inhibitory factor is present in the rheumatoid fluid.

Adult↗
PubMed1984

[Differential diagnosis of pleural effusions].

Pleural effusion is an important and common clinical finding. Pleural effusions can be readily obtained for analysis, and the examination of the cells therein is considered to be one of the most important diagnostic tools available for differentiating between malignant and non-malignant effusions. The present study was undertaken to test the diagnostic value of the determination of CEA in pleural fluid for a variety of diseases. Sixteen patients with pleural effusions were studied. Seven patients had carcinoma of the cervix, 7 of the ovary and 2 of the corpus. The positive rate of malignant cells was 81%. Among malignant effusions, only 44% of patients showed a CEA value above 10 mg/ml. This investigation suggests that cytological examination of pleural fluid is of considerable clinical significance for diagnosing the nature of pleural effusions, and effusion fluid CEA assay may provide a useful adjunct in the evaluation of effusion fluids for malignancy.

Adenocarcinoma↗
PubMed1984

[Studies on the relationship between quantitative histometry and obstetric complications].

Quantitative histometry of the placenta seems to make it possible to correlate the histopathological changes with various obstetric complications and disorders more precisely. In the present paper, the relationship between the histopathological changes in the placenta and the obstetric complications was investigated by quantitative histometry based on the standardized categories with uncomplicated normal cases as the controls. Among the obstetric complications, special emphasis has been placed on the pregnancies complicated by toxemia of pregnancy and diabetes mellitus. The obstetric disorders of postmature pregnancy and prolonged delivery have also been related to the quantitative histometry. It was revealed that the cytotrophoblast proliferation associated with the formation of syncytial knots was a very common finding both in toxemia and in these obstetric disorders. However, there was a great difference between the two in that impairment of the vasculature was highly specific in the placentae in the case of toxemia. The most conspicuous histopathological change recognized in the placentae due to diabetes was villous edema, which would be due to the osmotic imbalance. However, it is so unusual to find cases complicated by severe diabetes that typical villous edema is rarely observed. The quantitative approaches in the field of histopathology may leave some problems in that the specimens taken from the organ could not always be representative of the whole organ. However, quantitative histometry based on the fairy standardized categories has been shown to offer more promising information on the relationship between the histopathological changes in the placentae and the obstetric disorders.

Female↗
PubMed1983

[Viability of human chorionic villi in organ culture].

The method and technique of organ culture of human chorionic villi was elaborated in our laboratory. In this report, placental specimens obtained at 15 to 18 weeks of gestation were studied in organ culture for 7 days in terms of the maintenance of morphological integrity and the preservation of functions. The morphological aspect of the viability of the various villous elements with special emphasis on the trophoblast cells was described histologically and ultrastructurally. The functional aspect of the viability was discussed by analysis of the suppressive effect of the cultivated villi on plasminogen activator (PA) secretion by OK-432 elicited mouse peritoneal macrophages, by analyses of the activity of delta 5-3 beta-hydroxysteroid dehydrogenase coupled with delta 5-delta 4-isomerase (HSD) and of the radioactivity of [125I]-Iododeoxyuridine ( [125I]-IUdR) retained in the DNA of the trophoblast cells. Specimens of normal placenta were obtained at the time of induced abortion. The gestational ages were 15, 16 and 18 weeks. Specimens for organ culture were prepared under sterile conditions within one hour after expulsion of the placenta. Through gross dissection, the villi were isolated and minced into fragments of approximately 1 to 2 mm3. Incubation was carried out at 37 degrees C in a conventional static chamber with a gas mixture of 95% air, 5% CO2. The culture dishes and culture media were renewed every day. Data are the mean values of the duplicate incubations. In the first series of organ culture, placental fragments were removed at the end of each day of incubation, washed thoroughly and transferred to the new dishes with a culture medium freed from fetal bovine serum, where further incubation was performed for 24 hours. After this, placental fragments were fixed in 4% neutral buffered formalin, processed through paraffin embedding, serially sectioned at 5 micron and stained by periodic-acid Schiff (PAS) procedure. Culture medium obtained in this series at each end of incubation was put into a "macrophage plate" with the addition of plasminogen in the concentration of 2 IU/m1, in which OK-432 elicited mouse peritoneal macrophages with the capacity to secrete PA were cultured. Reaction was terminated at 24 hours unless otherwise indicated. Ten microliters of the medium in the "macrophage plate" was applied to the fibrin plate, and reaction was performed for 18 hours. PA activity was expressed by plasmin activity converted from plasminogen measured by the single radial immuno-diffusion method.(ABSTRACT TRUNCATED AT 400 WORDS)

Autoradiography↗
PubMed1983

Metabolism of 14C-pregnenolone in the placenta throughout pregnancy in organ culture.

The present study was undertaken to assess the metabolism of radiolabeled pregnenolone in the placenta throughout pregnancy in organ culture. The product generated from pregnenolone was only progesterone regardless of the weeks of gestation. The amount of progesterone formed by the placenta was 5.60 +/- 0.15 x 10(-11) moles/mg protein/hour in the 1st trimester (n = 4), 6.47 +/- 0.09 x 10(-11) moles/mg protein/hour in the 2nd trimester (n = 3) and 7.72 +/- 0.25 x 10(-11) moles/mg protein/hour in the 3rd trimester (n = 6). The increase in the formation of progesterone as gestation advanced was statistically significant. The increase in the placental weight throughout pregnancy was in proportion to the rise in maternal plasma levels of progesterone. These data indicate that the manifold increase in progesterone in the maternal circulation as gestation advances mainly reflects the increase in the functional mass of the placenta rather than the increased rate of production of progesterone in the placenta.

Carbon Radioisotopes↗
PubMed1982

Some biochemical properties of the preneoplastic antigen in rat liver hyperplastic nodules.

The biochemical properties of preneoplastic antigen (PN antigen) were investigated in order to resolve inconsistencies in previous reports. First of all, epoxide hydrase was purified biochemically from the liver of phenobarbital-treated rats and anti epoxide hydrase serum was prepared in rabbits. Immunochemically, in Ouchterlony double diffusion, PN antigen had the same antigenicity as epoxide hydrase. From an immunoprecipitation study using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and crossed immunoelectrophoresis, it was found that PN antigen may be composed of epoxide hydrase and at least one more protein (mol. wt. 27,000). However, the biochemical and biological properties of the protein binding to epoxide hydrase were not determined. The existence of PN antigen (epoxide hydrase and its binding protein) may be associated with microsomal membrane alterations during chemical carcinogenesis.

Animals↗