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Biomedical subjects

M Rust

Publications and source records attributed to M Rust.

At least 55 records · Page 3Linked to original sources
PubMed1983

Immunohistochemical studies on opioid peptides in the human placenta.

The demonstration of the immunoreactive proopiomelanocortin (POMC) peptides beta-endorphin (beta-E), beta-lipotropin (beta-LPH), alpha-melanotropin (alpha-MSH), and corticotropin (ACTH) in the human placenta or in tissue cultures of placental origin using radioimmuno-, bio-, or radioreceptor assay suggested that opioid peptide hormones or their precursors are synthesized in the placenta. In order to test this hypothesis, we tried to localize opioid peptides immunohistochemically. On neighbouring sections of the placenta which were incubated with antisera against beta-E, beta-LPH, alpha-MSH, ACTH, BAM-12 P, BAM-22 P, Heptapeptide, alpha-Neoendorphin and Dynorphin 1-17, the immunoperoxidase method was used. The placenta and the pituitary of perfusion-fixed rats were used for controls. The negative immunohistochemical findings in the human and rat placenta do not support the view that beta-E, beta-LPH, alpha-MSH and ACTH are synthesized in the syncytiotrophoblast of this organ. The site of origin of proopiomelanocortin peptides which are present in the maternal and the fetal plasma most likely is the maternal and fetal pituitary.

Animals↗
PubMed1981

Ion beam tritium labeling of proteins and peptides.

A general method for tritiating proteins, peptides, and other nonvolatile organic compounds has been developed. A carefully controlled particle beam composed of T3+ and T2+ ions and fast T2 molecules is accelerated into a sample target within a vacuum chamber. This beam method has been used to tritiate ribonuclease A, porcine pancreatic elastase, thermolysin, soybean trypsin inhibitor, alpha 1-protease inhibitor, and the peptide aldehydes leupeptin and antipain. After removal of all readily exchangeable tritium, the products were obtained in 32-83% yields with specific radioactivities of 18-856 Ci/mol. The products were carefully characterized, shown to be chemically pure, and to have complete biological activity. Simple tritium hydrogen exchange accounts for at least 82% of the reaction pathway with proteins and for 100% of the reaction with the peptide aldehydes. The ion beam method is a mild procedure for general tritium labeling of fragile protein macromolecules and other sensitive biological molecules.

Isotope Labeling↗
PubMed1981

[Treatment of pain and anesthesia in emergency and catastrophe medicine (author's transl)].

Catastrophe conditions for anesthesia and the treatment of pain arise when otherwise conventional techniques and procedures, including supervision of the patient, can only be carried out incompletely or not at all. Pain must be controlled with analgesics which have the least possible side-effects. When supplies of energy and gas fail, inhalation anesthesia can only be carried out under certain circumstances. In its place intravenous anesthesia can be used, with or without relaxation, with air breathing or artificial respiration. Reports in the literature of studies and experience attribute particular importance to ketamine in this respect.

Analgesics, Opioid↗
PubMed1981

[Changes of pressure within the middle ear during various kinds of anaesthesia (author's transl)].

Measurements of the middle ear pressure were taken during anaesthesia in 18 patients. Using nitrous oxide the middle ear pressure increased considerably. Other anaesthetics (i.e. anaesthesia with neuroleptics) did not show this remarkable change in middle ear pressure. To avoid complications by middle ear operations (tympanoplasty) we combine both methods of anaesthesia.

Acoustic Impedance Tests↗
PubMed1981

The role of endorphins during parturition.

The concomitant in vivo release of immunoreactive ACTH and beta-endorphin was investigated by measuring the plasma levels of both peptides in the plasma collected during labor and parturition of female human subjects. Maternal and fetal beta-endorphin circulation was investigated by estimating the plasma levels in umbilical vein and artery and comparing them with the maternal plasma content of the peptide. The peptides beta-LPH and epsilon beta-endorphin (which both react with the beta-endorphin antiserum) were separated chromatographically on Sephadex G-50. In the first, and particularly the second stages of labor, levels of both ACTH and beta-endorphin were significantly elevated. The beta-endorphin-like immunoreactivity was also elevated in the umbilical vein and artery, although these values were not as high as in the maternal vein. Neither oxytocin nor the mechanical stress produced by dilatation and curettage played a large role in releasing ACTH and beta-endorphin. The normal pathways of releasing pituitary trophic hormones (disinhibition of inhibitory control of releasing factors in the hypothalamus) must be assumed for the mothers, while fetal production of both beta-endorphin and beta-LPH was demonstrated.

Adrenocorticotropic Hormone↗
PubMed1981

[Neurophysiological correlates of spinal opiate analgesia (author's transl)].

The dorsal horn of the mammalian spinal cord contains interneurones which synthetize opioid peptides (endorphins) and release them on synaptic activation. Cells of origin of the spino-thalamic tract are in close functional relationship with these interneurones. The activation of the postulated endorphinergic mechanisms in the spinal cord could explain the selective reduction of painful stimuli following epidural application of opiate agonist.

Anesthesia, Spinal↗