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Biomedical subjects

S Dash

Publications and source records attributed to S Dash.

At least 91 records · Page 5Linked to original sources
PubMed1989

Granular cell variant of acute lymphoblastic leukaemia.

A rare morphological variant of acute lymphoblastic leukaemia in a ten years old child is described. Ninety five percent of the lymphoblasts had significant number of course granules in the cytoplasm. Diagnostic and prognostic significance of this variant is discussed.

Child↗
PubMed1988

The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays.

Genetic and molecular analysis has revealed a specific En-element of deletion derivative (En-I102) which reduces En/Spm-induced mutability. In the presence of En-I102 the excision frequency of both the autonomous En-1 element and the inhibitor element Spm-I5719A is reduced and excision occurs later in development. The 3697 bp long En-I102 element is derived from En-1 by an internal deletion of 4590 bp removing nucleotides 1862-6451. The promoter at the left end and sequences required for polyadenylation are retained in En-I102. It is transcribed to yield predominantly a 1.8 kb poly(A) RNA. cDNA analysis of this transcript indicated that it contains the coding capacity for a 386 amino acid polypeptide. This polypeptide shares homology with En/Spm encoded functions and we suggest that it interferes with transposition at the protein level.

Alleles↗
PubMed1988

Dysplastic eosinophils in three patients with acute promyelocytic leukaemia.

Three cases of acute promyelocytic leukaemia (M3) with dysplastic eosinophils are reported. Promyelocytes from these cases showed classical features of hypergranular acute promyelocytic leukaemia. All the cases had minimal organomegaly and disturbances of coagulation were minimal. All the cases were associated with dysplastic eosinophils in the marrow but very few of these eosinophils were found in peripheral smear. One of the three patients presented with a mass in the vertebral column, a feature not uncommonly seen in a variant form of AML-M2 with eosinophilia. Dysplastic eosinophils appear to arise from leukaemic clone itself. These three cases probably represent hitherto undescribed morphological variant of acute promyelocytic leukaemia where leukaemic cells show limited differentiation capability to dysplastic eosinophils.

Adult↗
PubMed1988

Biomonitoring of low levels of mercurial derivatives in water and soil by Allium micronucleus assay.

The Allium micronucleus (MNC) assay was developed to monitor low levels of mercury in aquatic and terrestrial environments. Four mercurial derivatives namely mercuric chloride (MC), methyl mercuric chloride (MMC), phenyl mercuric acetate (PMA) and a methoxy ethyl mercuric chloride based fungicide, Emisan-6, were tested to assess the sensitivity and versatility of the Allium MNC assay. Allium bulbs were set directly on water and soil contaminated with known levels of mercurial derivatives (0.0001-10.00 ppm). On the 5th day the endpoints measured were root length, mitoses with spindle abnormality and cells with MNC in root meristems. The effective concentrations of the test chemicals that cause 50% of root length as compared to control (EC50) were determined from dose-response curves so obtained. The lowest effective concentration tested (LECT) and highest ineffective concentration tested (HICT) for each of the mercurial derivatives for the induction of spindle malfunction and MNC were determined. It was found that EC50, LECT and HICT values for mercurial derivatives in soil were higher than those in water. The frequencies of cells with MNC and mitoses with spindle abnormality were highly correlated indicating that MNC is a good parameter of spindle malfunction. The present approach increased the sensitivity of the Allium assay by 10-fold, the detection limit being 0.001-0.1 ppm and 0.1-1.0 ppm in aquatic and terrestrial environments respectively, depending on the species of mercury.

Biological Assay↗