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S Hoshi

Publications and source records attributed to S Hoshi.

At least 127 records · Page 7Linked to original sources
PubMed1987

[Chromosome instability observed in cells from monospermic, homozygous moles].

The present study was undertaken to disclose the mechanism which concerned to the propensity to malignancy of complete moles. For this purpose, the incidence of chromosomal aberrations and sister chromatid exchanges(SCE) after the administration of 0-30 ng/ml mitomycin C(MMC) were compared among cells cultured from molar and normal villi, and peripheral blood. Abnormally high induction of chromosomal aberrations and SCE was observed in molar cells without MMC treatment when compared with those of the remaining two cells. Then, higher susceptible responses to the low concentration of MMC were also noticeable in molar cells rather than two kinds of cells. As a result, it was assumed that chromosome instability possibly played an important role for choriocarcinogenesis of molar cells. Homozygosity of paternally derived genes may be responsible for this chromosome fragility. Six chromosomal bands (1q 21, 2p 24, 2p 13, 7q 31, 8q 22, 13q 33) were preferentially involved in the formation of chromosomal aberrations occurred spontaneously in molar cells. Four of these six bands were identical with the break points necessary for the production of the marker chromosomes in various tumors described previously, though one band was consistent with the N-myc oncogene locus. Thus, the possible association between chromosome rearrangements occurred in those six bands and the choriocarcinogenesis was assumed, though further accumulation of cytogenetic and molecular data was absolutely necessary.

Chromosome Aberrations↗
PubMed1986

Expression of Leu-M1 antigens in carcinoma of the urinary bladder.

Anti-Leu-M1 antibody, which is well known to react with a human myelomonocytic antigen, was found to react with bladder carcinoma, but never with the normal bladder epithelium. Eighty-six cases of transitional cell carcinoma of the bladder were tested in sections of formalin-fixed and paraffin-embedded tissues by using an avidin-biotin immunoperoxidase technique. Fifty per cent of these cases were positive in Leu-M1, while the other three monoclonal antibodies (HBA4, HBG9 and HBH8), which were produced against a human bladder carcinoma cell line KU-1, reacted in 17 per cent, 27 per cent and 64 per cent of these cases respectively. Reactivities of these antibodies to 33 cases of normal bladder epithelium were Leu-M1, 0 per cent; HBA4, 0 per cent; HBG9, 0 per cent and HBH8, 30 per cent, respectively. Expression of Leu-M1 antigens in bladder epithelium seems to be specific for malignant change and highly frequent in the carcinoma patients. The relation between Leu-M1 expression and a degree or stage in bladder carcinomas, however, was not significant in our studies.

Animals↗
PubMed1986

Asialogangliosides as differentiation markers of rat erythropoietic and granulopoietic cells in rat bone marrow. Flow cytometric analysis of rat bone marrow cells using anti-asialoganglioside antibodies.

Flow cytometric analysis using anti-glycolipid antiserum was used on rat bone marrow cells to determine the relation between the glycolipid species expressed on cell surfaces and cell differentiation. Four kinds of antibodies against gangliotriaosylceramide (Gg3Cer), gangliotetraosylceramide (Gg4Cer), fucogangliotetraosylceramide (IV2 alpha Fuc-Gg4Cer) and IV3 alpha Gal-fucogangliotetraosylceramide (IV3 alpha GalIV2 alpha Fuc-Gg4Cer, blood group B lipid) were used. The cells sorted out by each anti-glycolipid antiserum were stained with May-Grünwald-Giemsa reagent and identified by microscopy. In the erythropoietic group, only polychromatic erythroblasts had these four glycolipids on their cell surfaces; none appeared on differentiated erythrocytes. These glycolipids were expressed during the early stages of immature granulocytes, especially in the promyelocyte and myelocyte stages of eosinophilic and neutrophilic granulocytes. Very limited populations of lymphocytes were sorted out as asialoganglioside-expressing cells. We concluded that asialogangliosides are useful differentiation markers for the erythropoietic and granulopoietic cells of rat bone marrow, and that anti-asialoganglioside antibody-flow cytometry is a very useful technique with which to isolate immature granulocytes and erythropoietic cells from rat bone marrow cells.

Animals↗
PubMed1985

[Prostatic tissue levels of ceftizoxime].

The concentration of Ceftizoxime (CZX) was determined in the prostatic tissue and serum of 130 patients with benign prostatic hypertrophy. Two grams of CZX was given by intravenous injection prior to TUR. The mean value of CZX levels in prostatic tissue and prostatic level/serum levels ratio (p/s ratio) after administration were 47.2 +/- 2.8 micrograms/g, 49.1% at 30 minutes, 33.3 +/- 2.2 micrograms/g, 51.4% at one hour, 22.2 +/- 2.7 micrograms/g, 60.8% at 2 hours, 13.6 +/- 3.9 micrograms/g, 64.2% at 4 hours, 3.04 +/- 0.54 micrograms/g, 74.5% at 8 hours, respectively. In conclusion, the concentration of CZX in the prostatic tissues attained the minimal inhibitory concentration of 80% for the gram-negative bacteria, the excluding P. aeruginosa. Thus clinical effectiveness of CZX could be expected on bacterial prostatitis and bacterial infection after prostatic operations.

Aged↗
PubMed1985

[Clinical statistics on testicular tumors in the Japanese during the 1970's].

A statistical analysis was made by computer on 511 cases of testicular tumor experienced at 14 facilities in Japan between 1970 and 1979. The age distribution of the patients had two peaks, one under 3 years (15%) and the other between 25 and 34 years (32%). Forty percent of the patients were office workers and 76% were college graduates. Fifty percent of the patients had 0 to 1 sibs. The blood type distribution was similar to that for the whole Japanese population. Six percent of the patients had a past history of trauma, and no relation with cell type was detected. Sixty nine percent of the patients were married and 31% were not married. Sixty seven percent of the patients over 30 had seminoma, and 78% of the patients under 29 years old had non-seminoma. Seminoma was rare in patients under 9 years old. Histologically, 75% were simple type, and 25% were mixed type. Forty three percent of the cases were seminoma and 57% were non-seminoma. Of the stage I cases, 78% and 57%, and of the stage III cases 7 and 28%, respectively, were seminoma and non-seminoma, many of the non-seminoma being at high stage. The 5-year survival rate for the 75 patients under 9 years old was 96%, and that for the 341 patients over 15 years was 70%, the survival rate for the patients under 9 years being significantly higher. The 5-year survival rate for stage I, II and III seminoma was 100, 65 and 0%, respectively, while that for non-seminoma was 93, 56 and 8%, respectively. The survival rate for stage I was higher for seminoma cases than for non-seminoma cases. No difference was detected between survival rate for stage II or III between seminoma and non-seminoma. The survival rate for seminoma did not differ with the time when the surgery was performed, but for the patients with non-seminomatous tumors, the survival rate was higher for the patients operated within one month than those operated later. The survival rate was not related to the weight of the extracted testicle. The 5-year survival rate for the patients with normal body temperature was 78% whereas that for the patients who had fevers was 32%. The 5-year survival rate for the patients not accompanied by abdominal tumors was about 80%, whereas that for the patients with palpated abdominal tumors was 42%.(ABSTRACT TRUNCATED AT 400 WORDS)

Adolescent↗