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S R Pattyn

Publications and source records attributed to S R Pattyn.

At least 109 records · Page 6Linked to original sources
PubMed1981

Parameters influencing the in vitro growth of Mycobacterium lepraemurium.

Investigations were done on the role of several parameters of incubation, carbon sources, fatty acids and Tween 80 on the in vitro growth of M. lm on Ogawa medium. It was found that incubation of aerated tubes at 37 degree C in a humidified atmosphere was optimal. Glycerol is necessary in the medium at concentrations of 0.5 to 2%. Acetate inhibits growth; there is no beneficial effect from Tween 80 or sodium salts of oleic, caproic, caprylic and palmitic acid. The pH of the medium is very critical since optimal growth occurs only between pH 5.8 and 6.3 on Ogawa egg yolk medium.

Culture Media↗
PubMed1981

Activity of the combination of isoniazid, protionamide and dapsone against Mycobacterium leprae and some other mycobacteria.

No additive effect was found at the addition of isoniazid to either dapsone or protionamide against M. leprae and against highly dapsone-sensitive in vitro growing Mycobacterium sp. strains. It is concluded that the indication of the administration of isoniazid in leprosy is extremely limited and could only be envisaged as a companion drug in combined therapy of dapsone-resistant multibacillary leprosy in light-skinned people, when aminoglycosides cannot be administered.

Animals↗
PubMed1980

In vitro cultivation and characterization of Mycobacterium lepraemurium.

Three different strains of M. lm were regularly grown in vitro from suspensions of mouse organs if at least 10(5) organisms were inoculated on Ogawa egg yolk medium and incubated at 35 degrees C in a humidified, CO2 enriched atmosphere. Growth is slow and requires 2-3 months. Colonies are 1-2 mm in diameter, white to pale yellow. Microscopically the bacteria are acid-alcohol-fast pleomorphic rods with branchings and beaded filamentous forms. Mice inoculated with in vitro grown subcultures develop an infection indistinguishable from the one observed after injection with mouse passage strains of M. lm. The in vitro characteristics of the strains are identical and different from all other known mycobacteria.

Animals↗
PubMed1980

Multivariate analysis of the activity of rifamycins against rapidly growing Mycobacteria, to define an in vitro screening model for their activity against mycobacterium leprae.

In an attempt to select a group of rapidly growing Mycobacteria which could serve as a model for the in vitro screening of rifamycin derivatives for their activity against Mycobacterium leprae, the MIC values of 237 strains of rapidly growing Mycobacteria were determined for 4 reference rifamycins with known activity against M. leprae and 19 rifamycins with unknown activity against M. leprae. Study of the results by a multivariate statistical technique, the principal components analysis, defined a group of 16 rapidly growing Mycobacteria (7 strains of M. phlei, 4 strains of M. thermoresistible, 3 strains of M. fortuitum and 2 strains of M. vaccae) showing the lowest distance to M. leprae. The analysis detected among rifamycin derivatives with different activities M. tuberculosis, compounds which should be active against M. leprae, and allows the selection of a limited number of rifamycins for the in vivo screening in the mouse model.

Animals↗
PubMed1979

Comparison of proteins from Mycobacterium fortuitum, Mycobacterium nonchromogenicum and Mycobacterium terrae using flat bed electrophoresis.

Polyacrylamide gel electrophoresis of bacterial lysates in a flat bed gives a linear relationship between 1n mol. wt of the proteins and the square root of their migration distances, thereby allowing standardization of different electrophoresis runs and precise comparison between homologous bands. The results obtained with Mycobacterium fortuitum, M. terrae and M. nonchromogenicum strains were used in numerical analysis. Mycobacterium fortuitum and M. nonchromogenicum showed a greater internal similarity than M. terrae, while two strains of the latter clustered with M. nonchromogenicum. The method described allows the comparison of mycobacteria with different generation times and provides a large number of good characters for numerical taxonomy.

Bacterial Proteins↗