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Biomedical subjects

T L Zaets

Publications and source records attributed to T L Zaets.

At least 37 records · Page 2Linked to original sources
PubMed1983

[Effect of water-soluble antioxidants on the permeability of lysosomal membranes and on the structure of the liver in rats with thermal burns].

Experiments on rats were made to study the effect of water-soluble antioxidants on the permeability of lysosomal membranes of liver cells and liver structure under burn. Antioxidants were injected intraperitoneally shortly after burn, whereas examination was performed after one day. It has been discovered that one day after burn there takes place an appreciable destabilization of lysosomal membranes with the release of a lysosomal matrix enzyme, cathepsin D to the cytoplasm. Liver structure had undergone substantial changes by that time. After administration of water-soluble antioxidants lysosomal membranes got stabilized while liver structure manifested but insignificant disorders.

Animals↗
PubMed1982

[Action of a high-molecular toxin from burned skin on the hepatocyte lysosomal apparatus].

Experiments on rats were made to study the action of toxic glycoprotein isolated from burn skin on the lysosomal apparatus of hepatocytes and morphological structure of the liver. It was shown that 2-2.5 h after intraperitoneal injection to normal rats of toxic preparations of varying purity, the animals developed morphological alterations similar to those seen in the liver of the burn animals. At the same time there was an appreciable increase in the percentage of unsedimentable activity of catepsine D of the total one, thereby indicating the toxin-induced injury to the lysosomal apparatus of hepatocytes.

Animals↗
PubMed1980

[Alteration of the permeability and composition of liver cell lysosomal membranes after a thermal burn].

Rat hepatocyte fractions enriched with lysosomes were studied one hour and 7 days after vast thermal burn of the skin. The ratio of free catepsine D activity to its total activity served as an index of lysosomal membrane resistance. The lipid composition was assayed by thin layer chromatography on silica gel. Material increase in lysosomal membrane permeability was recorded both one hour and 7 days after burn. Significant shifts were also seen in the composition of neutral lipids and phospholipids. Burn gave rise to the triglyceride fraction that was not present in the control. Among phospholipids the most labile were minor fractions, that is those of sphyngomyelin, phosphatidylinosite and phosphatidylserine. The changes in the lipids are considered as essential for destructive alterations in the liver in burn disease.

Animals↗
PubMed1977

[Inhibitors of trypsin-like proteolytic enzymes as means of preventing the development of secondary necrosis in burn wounds].

The effect of proteolytic inhibitor contrical on the experimental burn wound healing was studied in rats using biochemical, histological and histochemical methods. In control untreated animals with flame burn of 20% of body surface wound healing was associated with development of secondary necrosis, marked inflammatory reaction, augmented activity of proteases and peptidases. The use of contrical prevented secondary necrosis; this effect was apparently related to reduction of tissue proteolytic enzymes activity.

Acid Phosphatase↗
PubMed1977

[Experimental study of the immunotherapy of burns].

Experiments were conducted on rats. The influence of the serum of a burn convalescent on the toxic properties, the level of proteolytic enzymes activity, and morphological changes following burn were studied. After the burn the blood serum and the extracts of the organs acquired toxic properties; there was an increase in the activity of proteolytic enzymes, and marked morphological changes developed Administration of the serum of aburn convalescent promoted detoxication, diminished the activity of proteolytic enzymes distinctly, and decreased the morphological disturbances.

Animals↗
PubMed1977

[Characteristics of the lactate dehydrogenase isoenzyme spectrum of the skin in the presence of thermal lesions].

Experiments were conducted on rats; a study was made of the activity of lactic dehydrogenase (LDH) and its isoenzymes in the zone of affection and in the adjacent areas of the skin at various periods after the burn infliction; on the 1st--8th day there occurred a reduction of the sum total LDH activity in the scab zone and the underlying tissue by 70--80%, and in the margin and the intact skin--by 50%. These changes were accompanied by shifts in the isoenzymatic LDH spectrum in the affected tissue; the activity of fraction 5 displayed a sharp rise on the 1st day and that of fractions 2 and 3--a reduction, on the contrary, by the 8th day there was seen some diminution of fraction 5 activity and an elevation of fractions 2 and 3 activity. The following picture is observed on the 14th--22nd day after the burn; the sum total LDH activity remains low, the isoenzymatic LDH spectrum in the margin and the scab is largely normalized, whereas in the underlying tissue there persist changes in the LDH enzyme ratio (a reduction of fractions 1--3 activity, and a rise of fraction 5 activity).

Animals↗
PubMed1976

[Toxicity and proteolysis in gnotobiotic rats with thermal burns].

Experiments were conducted on germ-free (gnotobiotic) rats. A study was made of the toxic properties of the organs and the activity of proteolytic enzymes in them after thermal burns. Despite the absence of pathogenic microbial flora the germ-free rats developed the state of toxemia with an increase of the level of proteolytic enzymes. The extent of the toxic and catabolic reaction depended on the severity of the burn. Intoxication and an increase in proteolysis in the gnotobiotic rats were similar, and, in a number of cases greater than in usual animals. A conclusion was drawn on the important role of the tissue sources of intoxication in burns. A correlation between the toxemia and proteolysis was established.

Animals↗