Biomedical subjects

V Briese

Publications and source records attributed to V Briese.

At least 73 records · Page 4Linked to original sources

PubMed1989

[Detection of carcinoembryonic antigen (CEA) in cervico-vaginal irrigation fluids].

CEA estimations are effected in serum samples and in cervico vaginal fluids (n = 144) of patients with cervical condylomas, cytological undecided and cytological positive findings and in follow up performed histology. 21 healthy women were used as a control group. For taking cervico-vaginal fluids 5 ml isotonic NaCl solution were instilled in the fornix vaginae. After that 5 ml of the solution mixed with cervico vaginal fluids were aspirated. A polyclonal anti-CEA-antiserum was applied to radioimmunoassay. Remarkable high concentrations were found in cervico vaginal fluids compared with serum values. (Table: see text). In cases of cervical condylomas and cervical intraepithelial neoplasia significant increased CEA levels were determined in fluids compared with the control group. These results must be seen in consideration that the local CEA concentrations are scattered over a brand range. Regarding to CEA serum concentrations only slight increased values were found in cases of CIN I/II. (table; see text) Therefore CEA-serum estimations are not a diagnostic tool in CIN. The normal range of 10 micrograms/l was exceeded in only one sample of CIN II.

Adenocarcinoma↗

PubMed1989

[Placental protein 12 (PP 12) in cyst and peritoneal fluids and serum samples of patients with ovarian cysts and cystic ovarian tumors].

Placental Protein 12 (PP 12) concentrations were measured by a radioimmunoassay in cyst and peritoneal fluids and in serum samples respectively of patients with tumor like conditions and benign and malignant cystic ovarian tumours. PP 12 can not recommended as a serum tumour marker in ovarian cancer. It must be proved the value of PP 12 in follow up of ovarian cancer with help of more extensive sample material. There are many cysts in tumour like conditions with measurable PP 12-concentrations.

Ascitic Fluid↗

PubMed1988

Clinical evidence of secretory IgA and Schwangerschaftsprotein 1 in pleural effusions of lung cancer patients.

Secretory immunoglobulin A, alpha 1-fetoprotein, orosomucoid and alpha 2-macroglobulin have been estimated in pleural effusions of 11 lung cancer patients. Secretory immunoglobulin could be detected in all cancer patients except one with a secondary embolism. Levels could be found between 0.19 and 0.73 g/l. No SIgA could be detected in a control group of 7 patients with pleural effusions of other origin.

Enzyme-Linked Immunosorbent Assay↗

PubMed1988

[Detection of immune complexes in intrauterine retardation].

We tried to find an additional diagnostic parameter for intrauterine growth retardation. The immune complex estimations were carried out by means of precipitation of immune complexes with Polyethylenglykol 6.000 in 203 primi and multiparae suspected for intrauterine growth retardation. The control group (y = 0.073 X + 0.372; r = 0.459) showed an increase of immune complex concentrations towards the end of pregnancy. A possibility for interpretation is the daily penetration of fetal material in to the maternal circulation. Women with suspicion of intrauterine growth retardation (y = -0.045 X + 1.61; r = -0.053) showed a decrease of immune complex concentration at the end of pregnancy. This course is contrary to that one of immune complex concentrations in the control group. The distribution of immunglobulins in the precipitate is corresponding to the proportions in serum. We found mainly IgG and IgM, but also high molecular serumproteins as Alpha-2-Makroglobulin and Orosomucoid. A discreet correlation between intrauterine growth retardation and pathological immune complex values may be present.

Adolescent↗

PubMed1988

[Endometrium proteins].

Research in animal and human reproduction attends with proteins of the endometrium increasingly. Especially in the time of the blastocyst implantation and during survival of the fetal allograft the protein pattern of the endometrium could be important. In the development of the blastocyst of rabbits uteroglobin rendered as a sensitive marker for the biological activity of progesterone. In animal models the desynchronisation of the uteroglobin secretion demonstrated the inhibition of the implantation. In human an alpha 2-globulin was found represented an analogon regarding to rabbits uteroglobin possibly. In the female genital host defense mechanisms of the mucosal barriere especially local produced immunoglobins are important. During pregnancy decidual suppressor factors the maternal tolerance of the fetus.

Animals↗

PubMed1988

[Placental protein 5 and pregnancy zone protein in ovarian cysts and benign cystic ovarian tumors].

Placental protein 5 (PP 5), a serine protease inhibitor, was measured by radioimmunoassay in benign ovarian cyst fluids, especially in follicular and luteal fluids. PP 5 was detectable in 12 out of 29 cystic fluids; the levels ranging from greater than 1.0 to less than 100 micrograms/l. PP 5 was not detectable in the corresponding serum samples. Protein of the "pregnancy zone" (PZ) was found in 3 out of 11 follicular fluids (9-38 mg/l). Local production of PP 5 and PZ in cystic epithelia of the ovary was taken into consideration.

Female↗

PubMed1988

[CEA determination in aspired fluid of cystic ovarian tumors and ovarian cysts and in ascites and serum samples of patients with ovarian tumors].

Carcinoembryonic antigen (CEA) was assayed by radioimmunoassay in serum of 47 patients with cystic ovarian tumors, in 51 cases of cyst fluids of ovarian tumors and ovarian cysts, and in 16 cases of peritoneal effusions (ascites). In order to differentiate between cystic tumors and retentional cysts there was found, in consideration of a changed prevalence (0.65), a sensitivity of 55% and a specificity of 94%. The predictive value in regard to a positive assay result was 94% and in regard to a negative one was 53%. Elevated CEA-levels in ovarian tumor cyst fluids are mainly diagnostic for mucinous ovarian tumors. In regard to elevated CEA-levels in cyst fluids of ovarian tumors it is claimed the laparotomy and histological investigation.

Ascitic Fluid↗

PubMed1988

[Tumor markers in gynecology. General review].

Carcinoembryonic antigen (CEA) and alpha 1-fetoprotein (AFP) should like to be a part of the diagnostic tool of breast and ovarian cancer. HCG is an established specific tumor marker in the monitoring of trophoblastic tumors. The development of a specific HCG-beta-radioimmunoassay led off the possibility to determine the so called subclinical estimation of the tumor tissue. First clinical steps of tissue polypeptide antigen (TPA), cancer antigen 12-5 (CA-12-5), cancer antigen 15-3 (CA-15-3) and D-Dimer would be of great interest to study the correlation between serum concentrations and tumor mass. The following classification is showing other tumor markers in a general review, too dividing molecules produced from the tumor cell itself, so called tumor derived products, and on the other side tumor associated products accompanying the malignancy.

Biomarkers, Tumor↗

PubMed1988

[Protein studies in cystic and peritoneal fluids of ovarian tumors with special reference to CEA].

The levels of total protein, Ig, AFP, beta-HCG, CEA, SC, SP1 and alpha 2-PAG were measured in cystic and peritoneal effusions obtained from 70 females with benign and malignant ovarian tumors. This study was carried out to test out parameters those are useful for tumor monitoring of ovarian cancer. Our results indicate that determining CEA in peritoneal effusions could be one of useful methods in tumor monitoring of mucinous cystadenocarcinomas in cases of known histology and praetherapeutic estimated CEA levels in cystic or peritoneal effusions. Our results demonstrate that CEA-levels greater than 100 micrograms/l are mostly found in mucinous cystadenocarcinomas and in mucinous cystadenomas, too. High cystic CEA levels support the histology aiming for demonstration of mucinous component.

Adolescent↗

PubMed1987

Influenza specific antibodies in the female genital tract of mice after oral administration of live influenza vaccine.

In order to investigate the possible stimulation of antibodies in the genital tract by immunization female NMRI-mice were given orally a live influenza vaccine (A/PR/8/34, H1N1) on two occasions which were 10 days apart. Subsequently, virus specific IgA antibodies measured by an enzyme immunoassay in homogenates of urinary bladder, uterus and vagina and also in uterine washings. Specific IgA antibodies were not detectable in the sera of immunized mice. The high IgA titer in uterine washings, and in the homogenates suggests enhancement by vaccine of IgA antibody production in the genital tract.

Administration, Oral↗

PubMed1987

Placental protein 5 in benign and malignant ovarian cyst fluids.

Placental protein 5 (PP5), a serine protease inhibitor, was measured by radioimmunoassay in fluids aspirated from benign and malignant cystic ovarian tumours. PP5 was found in 7 out of 9 malignant and in 12 out of 29 benign cyst fluids (p less than 0.01), the levels ranging from 3.0 to 73.4 micrograms/l. No PP5 was found in the corresponding serum samples from 4 patients whose malignant cystadenocarcinomas contained PP5. In those tumours where PP5 was found, no difference was observed in the PP5 levels between benign and malignant, or between serous and mucinous tumours.

Female↗

PubMed1987

[Pregnancy protein-1 in the serum of patients with diseases of the breast].

Pregnancy-specific beta 1-glycoprotein (SP1) was assayed by enzyme immunoassay in serum from 32 patients with breast diseases (11 mastopathies, 6 benign tumors and 15 breast cancers). Healthy blood donors were used as control group. There were significant differences between the SP1-serum concentrations of patient group (mastopathies, benign tumors, breast cancer) and the control group (p less than 0.001). The SP1-values of the groups with mastopathies, benign tumors and breast cancer did not differ significantly. Elevated SP1-concentrations were found in 4 of 11 patients with mastopathy, in 3 of 6 patients with benign tumor and in 10 of 15 patients with breast cancer versus 1 of 24 blood donors. With the help of SP1 determination in serum of 5 breast cancer patients before operation and 6 months post operation we did not found a correlation with further clinical course.

Adenocarcinoma, Scirrhous↗

PubMed1987

[Immunology of fetomaternal interaction: HLA antigens].

The histocompatibility complex is an essential part of the immunology of the fetomaternal interactions. HLA antigen haplotyps are present on fetal lymphocytes. HLA antigens are also localized in the chorion. The paternal HLA antigenicity is decreased by different mechanism. In multiparae HLA antibodies are detectable in 20 to 30 per cent without injuries of the fetal tissues or wellbeing respectively. The placenta possesses the ability of the immunoadsorption of maternal lymphocytotoxic antibodies. In cases of increased compatibility of materno-paternal HLA antigens habitual abortions are more frequent according to some authors. Therapeutic leucocyte transfusion can be used regarding to prevention of recurrent abortions.

Abortion, Habitual↗

PubMed1987

[Regulation of the immunoglobulin content of the mucous membranes of the uterus: effect of estradiol on immunoglobulin A content].

A was used for the animal experimental investigations standard castration mouse model. Vaginal smears indicated no cyclic changes 14 days after the removal of the ovaries. 12.5 micrograms estradiol benzoate have been injected intramuscularly in this time. 3 days later uterus lavage fluids and tissue homogenates were used for the IgA-test, single radial immunodiffusion. The standard was prepared from mouse colostrum because S-IgA is the dominant IgA in secretions. The results were expressed as U/l selected arbitrary. Our findings of significant increased IgA concentrations in uterus lavage fluid after estradiol stimulation and, in contrary, no changed IgA concentrations in homogenates support the hypothesis, that the secretory component synthesis in the epithelial cells of the uterus is controlled by estrogens.

Animals↗

PubMed1987

[Secretory immunoglobulin A (S-IgA) in the amniotic fluid supports the fetal maturity profile].

Amniotic fluid levels of secretory immunoglobulin A(S-IgA) were measured by single radial immunodiffusion according to the method of Mancini using a monospecific antiserum against the human secretory component. 114 amniotic fluid samples were examined. The S-IgA values showed a correlation (r = 0.49) with the phospholipid levels. Additionally, it was observed no correlation between Serum and amniotic fluid S-IgA concentrations estimated as parallel investigations in 20 patients. S-IgA values greater than or equal to 82 mg/l were used to diagnose a sufficient fetal lung maturity. False negative values of S-IgA are possible. In 19 cases of newborns without signs of respiratory distress syndrome amniotic fluid S-IgA estimations were performed up to 32 hours before the delivery. False negative phospholipid levels were estimated in 2 cases, false negative S-IgA levels in 4 cases. The measurement of S-IgA in amniotic fluid will serve as an additional parameter for the estimation of fetal lung maturity.

Amniocentesis↗

PubMed1987

[Behavior of some tumor markers (secretory immunoglobulin A, pregnancy-associated alpha 2-glycoprotein, circulating immune complexes in the serum of patients with breast tumors].

Concentrations of secretory immunoglobulin A (S-IgA), pregnancy associated alpha 2-glycoprotein (alpha 2-PAG) and circulating immune complexes (CIC) were estimated in serum samples of 20 blood donors, 14 patients with benign mamma tumors, 44 patients with mastopathies and 33 patients with breast cancer disease. The proteins were determined by means of the single radial immunodiffusion and the polyethylene glycol precipitation. S-IgA serum concentrations of the cancer group compared with those of the benign tumors, were increased significantly (benign tumors: mean = 40.27 mg/l; s = 15.61; carcinoma disease: mean = 78.87 mg/l; s = 72.80). alpha 2-PAG serum concentrations of the cancer-and benign tumor group as well as the mastopathy group were also increased significantly in comparison with the blood donor group (blood donors: mean = 143.75 mg/l; s = 198.50; benign tumors: mean = 295.85 mg/l; s = 232.64; mastopathies: mean = 190.86 mg/l; s = 177.77; carcinoma disease: mean = 192.52 mg/l; s = 172.88).

Adolescent↗

PubMed1987

[Detection of immunoglobulins in cervical secretions in an inflammatory adnexal process].

Single radial immunodiffusion test was used for estimations of IgG, M, A and secretory immunoglobulin A (S-IgA) in cervical secretions of patients with pelvic inflammations. Salpingo-oophoritis was diagnosed and staged by laparoscopy and an activity index aimed controlling the during therapy and decreased after therapy, but the decrease after therapy was significant for IgA only. Immunoglobulin values before and after therapy were as follows: IgG (mean = 0.63 g/l, s = 0.54, n = 32 and x = 0.24 g/l, s = 0.27, n = 7), IgM (mean = 0.028 g/l, s = 0.028, n = 33 and mean = 0.17 g/l, s = 0.023, n = 7), IgA (mean = 0.12 g/l, s = 0.09, n = 30 and mean = 0.04 g/l, s = 0.035, n = 7), S-IgA (mean = 0.77 g/l, s = 0.66 n = 30 and mean = 0.28 g/l, s = 0.41, n = 7). In no case we found an S-IgA deficiency. On the other side IgM could not be estimated in cervical secretions of 8 patients with long time applicated intrauterine device. Therefore IgM in cervical secretions may be a sign of acute ascending inflammations.

Adolescent↗

PubMed1987

[Detection of specific antibodies to influenza viruses of the genital tract of mice following oral immunization].

Balb c-mice were immunized orally twice within 10 days with a live influenza vaccine (A PR 8/34, H1 N1). Specific antibodies were determined in sera, uterus homogenates and uterus lavage fluids. In all compartments specific antibodies of IgG class were estimated by means of an enzymimmunoassay. Regarding estrogen cyclus of mice the antibody titers were divided into diestrus, proestrus, estrus and metestrus. We have not seen any cyclic changes of antibody titers. The results support the concept of a common immune mucosal system. The continuous antibody titers during genital cyclus are evident for using oral vaccination aiming to induce secretory antibodies in genital tract.

Administration, Oral↗