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PubMed · 10486528

Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis.

B S Edwards·F Kuckuck·L A Sklar
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Abstract

BACKGROUND: The tools for high throughput flow cytometry have been limited in part because of the requirement that the samples must flow under pressure. We describe a simple system for sampling repetitively from an open vessel. METHODS: Under computer control, the sample is loaded into a sample loop in a reciprocating eight-way valve by the action of a syringe. When the valve position is switched, the plug of sample in the sample loop is transported to the flow cytometer by a pressure-driven fluid line. By coupling the plug-forming capability to a second multi-port valve, samples can be delivered sequentially from separate vessels. RESULTS: The valve is able to deliver samples at rates ranging up to about 9 samples per minute. Each plug of sample has uniform delivery characteristics with a reproducible coefficient of variation (CV). Even at the highest sampling rate, carryover between samples is limited. CONCLUSIONS: Plug-flow flow cytometry has the potential to automate the delivery of small samples from unpressurized sources at rates compatible with many screening and assay applications.

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AnimalsAutomationCHO CellsCricetinaeFlow CytometryGreen Fluorescent ProteinsLuminescent ProteinsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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B S Edwards, F Kuckuck, L A Sklar. 1999-10-01. Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis.. https://pubmed.ncbi.nlm.nih.gov/10486528/

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