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Advanced technique for three-dimensional visualization of compound distributions in a rice kernel.

Y Ogawa·J Sugiyama·H Kuensting·T Ohtani·S Hagiwara·X Liu·M Kokubo·A Yamamoto·Kudoh Ki·T Higuchi
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Abstract

A three-dimensional (3D) visualization technique for the compound distribution in a rice kernel was developed. This technique is a combination of sectioning, staining, and digital image postprocessing. By using a special microtome system with adhesive tapes, a set of sequential sections of a rice kernel, which can be preserved with their own set of relative position data, was obtained. A single set of sequential sections was stained by various chemical techniques for the visualization of protein, starch, or lipid content. Each stained section was digitally captured using a CCD imaging device. As the stained areas represent areas containing dye-target complex, the distribution of each compound in the section was visualized in two dimensions. The digitally captured images of a single set of sequential sections were reconstructed to produce a 3D plotting image. As a result, the distributions of various compounds in a rice kernel could be visualized in a new 3D model.

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Y Ogawa, J Sugiyama, H Kuensting, T Ohtani, S Hagiwara, X Liu, M Kokubo, A Yamamoto, Kudoh Ki, T Higuchi. 2001. Advanced technique for three-dimensional visualization of compound distributions in a rice kernel.. https://doi.org/10.1021/jf000997p

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