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External quality assessment program for Chlamydia trachomatis diagnostic testing by nucleic acid amplification assays.

Sally Land·Sepehr Tabrizi·Anthony Gust·Elizabeth Johnson·Susan Garland·Elizabeth M Dax
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Abstract

We report the results from 57 Australian diagnostic laboratories testing two external quality assessment panels using either the Roche Amplicor Chlamydia trachomatis test (R-PCR) or the Abbott LCx Chlamydia trachomatis assay (A-ligase chain reaction [LCR]). Panel samples were either normal urine spiked with Chlamydia trachomatis antigen or clinical urine specimens. There was no significant difference between laboratories or between assays in detection of C. trachomatis-positive clinical samples. Only at the lower limit of detection of the assays did the R-PCR demonstrate increased sensitivity over the A-LCR in the detection of C. trachomatis antigen. However, it was found that single-sample testing could lead to decreased test sensitivity. Detection of the presence of inhibitors of nucleic acid amplification differed between laboratories.

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AustraliaChlamydia InfectionsChlamydia trachomatisHumansLaboratoriesLigase Chain ReactionNucleic Acid Amplification TechniquesPolymerase Chain ReactionQuality ControlReagent Kits, DiagnosticSensitivity and SpecificityUrineEvaluation StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Sally Land, Sepehr Tabrizi, Anthony Gust, Elizabeth Johnson, Susan Garland, Elizabeth M Dax. 2002. External quality assessment program for Chlamydia trachomatis diagnostic testing by nucleic acid amplification assays.. https://doi.org/10.1128/jcm.40.8.2893-2896.2002

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