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PubMed · 12191919

A comparison of imaging methods for use in an array biosensor.

Joel P Golden·Frances S Ligler
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Abstract

An array biosensor has been developed which uses an actively-cooled, charge-coupled device (CCD) imager. In an effort to save money and space, a complementary metal-oxide semiconductor (CMOS) camera and photodiode were tested as replacements for the cooled CCD imager. Different concentrations of CY5 fluorescent dye in glycerol were imaged using the three different detection systems with the same imaging optics. Signal discrimination above noise was compared for each of the three systems.

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Biosensing TechniquesCold TemperatureDiagnostic ImagingEquipment DesignFluoroimmunoassayMicroscopy, FluorescenceReproducibility of ResultsSemiconductorsSensitivity and SpecificityVideo RecordingNASA Discipline Life Sciences TechnologiesNon-NASA CenterComparative StudyEvaluation StudyJournal Article

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Joel P Golden, Frances S Ligler. 2002. A comparison of imaging methods for use in an array biosensor.. https://doi.org/10.1016/s0956-5663(02)00060-x

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