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PubMed · 12485759

Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection.

Ilaria Mannelli·Maria Minunni·Sara Tombelli·Marco Mascini
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Abstract

A DNA piezoelectric sensor has been developed for the detection of genetically modified organisms (GMOs). Single stranded DNA (ssDNA) probes were immobilised on the sensor surface of a quartz crystal microbalance (QCM) device and the hybridisation between the immobilised probe and the target complementary sequence in solution was monitored. The probe sequences were internal to the sequence of the 35S promoter (P) and Nos terminator (T), which are inserted sequences in the genome of GMOs regulating the transgene expression. Two different probe immobilisation procedures were applied: (a) a thiol-dextran procedure and (b) a thiol-derivatised probe and blocking thiol procedure. The system has been optimised using synthetic oligonucleotides, which were then applied to samples of plasmidic and genomic DNA isolated from the pBI121 plasmid, certified reference materials (CRM), and real samples amplified by the polymerase chain reaction (PCR). The analytical parameters of the sensor have been investigated (sensitivity, reproducibility, lifetime etc.). The results obtained showed that both immobilisation procedures enabled sensitive and specific detection of GMOs, providing a useful tool for screening analysis in food samples.

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Affinity LabelsAgrobacterium tumefaciensBiosensing TechniquesCaulimovirusCoated Materials, BiocompatibleCrystallizationDNADNA ProbesDNA, RibosomalElectrochemistryEquipment DesignFood ContaminationMicroelectrodesOrganisms, Genetically ModifiedPolymerase Chain ReactionQuality ControlQuartzReproducibility of ResultsSensitivity and SpecificityTransducersComparative StudyJournal Article

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Ilaria Mannelli, Maria Minunni, Sara Tombelli, Marco Mascini. 2003. Quartz crystal microbalance (QCM) affinity biosensor for genetically modified organisms (GMOs) detection.. https://doi.org/10.1016/s0956-5663(02)00166-5

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