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Cascade stacking and cascade electrofocusing: their interconversion and fundamental unity.

N Y Nguyen·D Rodbard·P J Svendsen·A Chrambach
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ComputersEvaluation Studies as TopicGelsHemoglobinsHumansHydrogen-Ion ConcentrationIsoelectric FocusingPhycoerythrinProteinsSerum Albumin, BovineComparative StudyJournal Article

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N Y Nguyen, D Rodbard, P J Svendsen, A Chrambach. 1977. Cascade stacking and cascade electrofocusing: their interconversion and fundamental unity.. https://doi.org/10.1016/0003-2697(77)90288-3

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Molecular orbital study on the glutathione-dependent detoxication of ozonides.

The present paper describes a theoretical study on the mechanism underlying the reaction of cellular glutathione (GSH) with polyunsaturated fatty acid ozonides. The reaction can be catalyzed by glutathione S-transferases and leads to detoxication of the ozonides. Semi-empirical molecular orbital computer calculations suggest that the reaction of glutathione with ozonides involves a nucleophilic attack at one of the carbon atoms of the ozonide ring, instead of at one of the peroxidic oxygen atoms of the ozonide ring. This implies a mechanism different from that of the glutathione S-transferase-mediated reaction with hydroperoxides, previously proposed for the glutathione-dependent detoxication of fatty acid ozonides.

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