PubMed · 14059588
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Y S SHEN. 1963-02-28. CHYLE PERITONITIS.. https://pubmed.ncbi.nlm.nih.gov/14059588/
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To evaluate the diagnostic performance of a commercially available Mycobacterium tuberculosis PCR assay (Amplicor MTB-ROCHE), 2296 respiratory and nonrespiratory specimens from 2296 patients with suspected tuberculosis (TB) were collected prospectively in an 8-year period. Clinical data for each patient were abstracted, and all samples were examined blindly by direct microscopy, culture, and PCR. M. tuberculosis DNA was detected in 93 of 113 culture-positive samples and in 29 of 38 samples from patients with probable TB. The lowest sensitivity was observed in pleural fluid and abscess aspirates. The sensitivity, specificity, and positive predictive value of the assay were 97.2%, 100%, and 100% for smear-positive specimens and 75.3%, 97.0%, and 47.5% for smear-negative specimens, respectively. The PCR cost per additional correct clinical decision was Euro 2826 but would have declined to Euro 308 if the test was applied only to smear-positive specimens. The overall performance of Amplicor MTB test was excellent for smear-positive, but suboptimal for smear-negative specimens.
Since its invasion into the field of small ion analysis, quantitation of trace ionic analytes has always been a challenging task for CE, especially when dealing with highly saline samples. This review summarizes the method's progress and significant developments in the area due to its combination with on-line preconcentration by transient ITP (tITP). Principles of tITP stacking in high-conductivity solutions and operational preconcentration modes are considered. The most important application areas covered in this review include the analysis of seawater and biological fluids (urine, serum, etc.). Examples cited in this review demonstrate that tITP-CE is now on a certain way of becoming a recognized technique when the determination of trace ionic species in loaded matrices is the aim.