Microfluidic device for single-cell analysis.

Aaron R Wheeler; William R Throndset; Rebecca J Whelan; Andrew M Leach; Richard N Zare; Yish Hann Liao; Kevin Farrell; Ian D Manger; Antoine Daridon

doi:10.1021/ac0340758

PubMed · 14570213

Microfluidic device for single-cell analysis.

Aaron R Wheeler·William R Throndset·Rebecca J Whelan·Andrew M Leach·Richard N Zare·Yish Hann Liao·Kevin Farrell·Ian D Manger·Antoine Daridon

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Abstract

We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.

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Calcium Cells Cells, Cultured Dimethylpolysiloxanes Fluorescent Dyes Humans Indicators and Reagents Jurkat Cells Microcomputers Microscopy, Fluorescence Nanotechnology Photochemistry Silicones U937 Cells Journal Article

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Aaron R Wheeler, William R Throndset, Rebecca J Whelan, Andrew M Leach, Richard N Zare, Yish Hann Liao, Kevin Farrell, Ian D Manger, Antoine Daridon. 2003-07-15. Microfluidic device for single-cell analysis.. https://doi.org/10.1021/ac0340758

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