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PubMed · 15007450

Sub-second isoelectric focusing in free flow using a microfluidic device.

Yi Xu·Chao-Xuan Zhang·Dirk Janasek·Andreas Manz
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Abstract

Using a microfabricated chip with a bed volume of 0.2 microL we demonstrate the validity of the scaling laws for molecular mass transport of isoelectric focusing (IEF) in free flow. Nano- or microlitre sample volumes can be concentrated within 430 ms by a factor of up to 400. These very fast performances make the chip applicable to proteomic analysis and for continuous monitoring of biochemical processes.

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Angiotensin IAngiotensin IIHydrogen-Ion ConcentrationInsulin-Like Growth Factor IIsoelectric FocusingMicrocomputersNanotechnologyPeptidesJournal ArticleResearch Support, Non-U.S. Gov't

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Yi Xu, Chao-Xuan Zhang, Dirk Janasek, Andreas Manz. 2003-10-07. Sub-second isoelectric focusing in free flow using a microfluidic device.. https://doi.org/10.1039/b308476k

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