Analyzing checkpoint controls in human skin.

Sandra Pavey; Brian G Gabrielli

doi:10.1385/1-59259-788-2:175

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Analyzing checkpoint controls in human skin.

Sandra Pavey·Brian G Gabrielli

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Abstract

A short-term whole-skin organ culture model has been established to enable the investigation of cell cycle perturbations in epidermal layer cells following exposure to ultraviolet radiation (UVR). This model affords the opportunity to manipulate the growth and nutrient conditions, and to perform detailed biochemical and immunohistochemical analysis of skin cells in their normal epidermal layer microenvironment. The use of this model is described in this chapter.

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Bromodeoxyuridine Cell Cycle Coloring Agents Culture Media Epidermis Formaldehyde Genes, cdc Humans Immunohistochemistry Male Organ Culture Techniques Skin Time Factors Ultraviolet Rays Journal Article Research Support, Non-U.S. Gov't

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Sandra Pavey, Brian G Gabrielli. 2004. Analyzing checkpoint controls in human skin.. https://doi.org/10.1385/1-59259-788-2%3A175

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