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Long-term changes in rabbit cornea after ionizing radiation.

Takahiro Kiuchi·Hideo Tatsuzaki·Taketoshi Wakabayashi·Fumiki Okamoto·Yuichi Kaji·Tetsuro Oshika
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Abstract

PURPOSE: To investigate long-term changes in the cornea after ionizing irradiation in rabbits. METHODS: Mature albino rabbits (n = 4) were unilaterally irradiated with 20 Gy of x-rays. The contralateral eye served as a control. The rabbits were examined with slit-lamp biomicroscopy for 5 years. The eyes were then enucleated for histopathologic examinations with light microscopy and transmission electron microscopy. RESULTS: On biomicroscopy, there was no corneal abnormality during the 5-year follow-up period. On histopathology, no abnormality was observed in the corneal epithelium. In the endothelium, a vacuole-like structure was recognized by light microscopy, and transmission electron microscopy revealed marked enlargement of intercellular space and anamorphosis of the nuclei. CONCLUSION: Moderate x-ray irradiation to rabbit eye did not induce any long-term damage to the corneal epithelium, but the endothelium demonstrated persistent and irreversible damage, which was observed even 5 years after irradiation.

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AnimalsCell DivisionCorneaEndothelium, CornealEpithelium, CornealRabbitsRadiation Injuries, ExperimentalRadiation, IonizingJournal Article

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Takahiro Kiuchi, Hideo Tatsuzaki, Taketoshi Wakabayashi, Fumiki Okamoto, Yuichi Kaji, Tetsuro Oshika. 2004. Long-term changes in rabbit cornea after ionizing radiation.. https://doi.org/10.1097/01.ico.0000136689.75028.d7

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