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Polarization-resolved second-harmonic-generation optical coherence tomography in collagen.

Brian E Applegate·Changhuei Yang·Andrew M Rollins·Joseph A Izatt
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Abstract

We describe a novel imaging technique, second-harmonic-generation optical coherence tomography (SHOCT). This technique combines the spatial resolution and depth penetration of optical coherence tomography (OCT) with the molecular sensitivity of second-harmonic-generation spectroscopy. As a consequence of the coherent detection required for OCT, polarization-resolved images arise naturally. We demonstrate this new technique on a skin sample from the belly of Icelandic salmon, acquiring polarization-resolved SHOCT and OCT images simultaneously.

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AnimalsCollagenEquipment DesignEquipment Failure AnalysisMicroscopy, PolarizationSalmonSkinSubtraction TechniqueTissue DistributionTomography, Optical CoherenceEvaluation StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Brian E Applegate, Changhuei Yang, Andrew M Rollins, Joseph A Izatt. 2004-10-01. Polarization-resolved second-harmonic-generation optical coherence tomography in collagen.. https://doi.org/10.1364/ol.29.002252

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