NMR-based metabonomic study of transgenic maize.

Cesare Manetti; Cristiano Bianchetti; Mariano Bizzarri; Lorena Casciani; Cecilia Castro; Giuseppe D'Ascenzo; Maurizio Delfini; Maria Enrica Di Cocco; Aldo Laganà; Alfredo Miccheli; Mario Motto; Filippo Conti

doi:10.1016/j.phytochem.2004.10.015

PubMed · 15561185

NMR-based metabonomic study of transgenic maize.

Cesare Manetti·Cristiano Bianchetti·Mariano Bizzarri·Lorena Casciani·Cecilia Castro·Giuseppe D'Ascenzo·Maurizio Delfini·Maria Enrica Di Cocco·Aldo Laganà·Alfredo Miccheli·Mario Motto·Filippo Conti

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Abstract

The aim of this research was to verify the possibility of identifying and classifying maize seeds obtained from transgenic plants, in different classes according to the modification, on the basis of the concerted variation in metabolite levels detected by NMR spectra. It was possible to recognise the discriminant metabolites of transgenic samples as well as to classify non-a priori defined samples of maize. It is important to underline that the obtained results are useful to point out the metabolic consequences of a specific genic modification on a plant, without using a targeted analysis of the different metabolites, in fact it was possible to classify the seeds also without the complete assignment of the spectra. The analysis was performed by applying multivariate techniques (principal component analysis and partial least squares-discriminant analysis) to NMR data.

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Gene Expression Genetic Variation Least-Squares Analysis Magnetic Resonance Spectroscopy Plant Proteins Plants, Genetically Modified Principal Component Analysis Seeds Zea mays Comparative Study Journal Article

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Cesare Manetti, Cristiano Bianchetti, Mariano Bizzarri, Lorena Casciani, Cecilia Castro, Giuseppe D'Ascenzo, Maurizio Delfini, Maria Enrica Di Cocco, Aldo Laganà, Alfredo Miccheli, Mario Motto, Filippo Conti. 2004. NMR-based metabonomic study of transgenic maize.. https://doi.org/10.1016/j.phytochem.2004.10.015

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