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PubMed · 15846362

Standardizing global gene expression analysis between laboratories and across platforms.

Theodore Bammler·Richard P Beyer·Sanchita Bhattacharya·Gary A Boorman·Abee Boyles·Blair U Bradford·Roger E Bumgarner·Pierre R Bushel·Kabir Chaturvedi·Dongseok Choi·Michael L Cunningham·Shibing Deng·Holly K Dressman·Rickie D Fannin·Fredrico M Farin·Jonathan H Freedman·Rebecca C Fry·Angel Harper·Michael C Humble·Patrick Hurban·Terrance J Kavanagh·William K Kaufmann·Kathleen F Kerr·Li Jing·Jodi A Lapidus·Michael R Lasarev·Jianying Li·Yi-Ju Li·Edward K Lobenhofer·Xinfang Lu·Renae L Malek·Sean Milton·Srinivasa R Nagalla·Jean P O'malley·Valerie S Palmer·Patrick Pattee·Richard S Paules·Charles M Perou·Ken Phillips·Li-Xuan Qin·Yang Qiu·Sean D Quigley·Matthew Rodland·Ivan Rusyn·Leona D Samson·David A Schwartz·Yan Shi·Jung-Lim Shin·Stella O Sieber·Susan Slifer·Marcy C Speer·Peter S Spencer·Dean I Sproles·James A Swenberg·William A Suk·Robert C Sullivan·Ru Tian·Raymond W Tennant·Signe A Todd·Charles J Tucker·Bennett Van Houten·Brenda K Weis·Shirley Xuan·Helmut Zarbl·Members of the Toxicogenomics Research Consortium
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Abstract

To facilitate collaborative research efforts between multi-investigator teams using DNA microarrays, we identified sources of error and data variability between laboratories and across microarray platforms, and methods to accommodate this variability. RNA expression data were generated in seven laboratories, which compared two standard RNA samples using 12 microarray platforms. At least two standard microarray types (one spotted, one commercial) were used by all laboratories. Reproducibility for most platforms within any laboratory was typically good, but reproducibility between platforms and across laboratories was generally poor. Reproducibility between laboratories increased markedly when standardized protocols were implemented for RNA labeling, hybridization, microarray processing, data acquisition and data normalization. Reproducibility was highest when analysis was based on biological themes defined by enriched Gene Ontology (GO) categories. These findings indicate that microarray results can be comparable across multiple laboratories, especially when a common platform and set of procedures are used.

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Gene Expression ProfilingLaboratoriesOligonucleotide Array Sequence AnalysisReproducibility of ResultsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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Theodore Bammler, Richard P Beyer, Sanchita Bhattacharya, Gary A Boorman, Abee Boyles, Blair U Bradford, Roger E Bumgarner, Pierre R Bushel, Kabir Chaturvedi, Dongseok Choi, Michael L Cunningham, Shibing Deng, Holly K Dressman, Rickie D Fannin, Fredrico M Farin, Jonathan H Freedman, Rebecca C Fry, Angel Harper, Michael C Humble, Patrick Hurban, Terrance J Kavanagh, William K Kaufmann, Kathleen F Kerr, Li Jing, Jodi A Lapidus, Michael R Lasarev, Jianying Li, Yi-Ju Li, Edward K Lobenhofer, Xinfang Lu, Renae L Malek, Sean Milton, Srinivasa R Nagalla, Jean P O'malley, Valerie S Palmer, Patrick Pattee, Richard S Paules, Charles M Perou, Ken Phillips, Li-Xuan Qin, Yang Qiu, Sean D Quigley, Matthew Rodland, Ivan Rusyn, Leona D Samson, David A Schwartz, Yan Shi, Jung-Lim Shin, Stella O Sieber, Susan Slifer, Marcy C Speer, Peter S Spencer, Dean I Sproles, James A Swenberg, William A Suk, Robert C Sullivan, Ru Tian, Raymond W Tennant, Signe A Todd, Charles J Tucker, Bennett Van Houten, Brenda K Weis, Shirley Xuan, Helmut Zarbl, Members of the Toxicogenomics Research Consortium. 2005-04-21. Standardizing global gene expression analysis between laboratories and across platforms.. https://doi.org/10.1038/nmeth754

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