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Variable low-mass filtering using an electrodynamic ion funnel.

Jason S Page·Aleksey V Tolmachev·Keqi Tang·Richard D Smith
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Abstract

An adjustable low-mass filter has been developed for an electrospray ionization (ESI) source to block ions associated with unwanted background species from entering the mass spectrometer. The low-mass filter is made by using an adjustable potential energy barrier from the conductance-limiting plate of an electrodynamic ion funnel, which prohibits species with higher ion mobilities from exiting the ESI source. We show that this arrangement provides a linear voltage adjustment for low-mass filtering from m/z 0 to 500. Mass filtering above m/z 500 is also performed; however, higher-mass species are attenuated. The mass filter was tested with a liquid chromatography/mass spectrometry (LC/MS) analysis of a bovine serum albumin (BSA) tryptic digest and resulted in the ability to block low-mass, background species, which accounted for 40-70% of the total ion current immediately behind the ESI source during peak elution and detection.

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AnimalsCattleChromatography, LiquidPeptidesSerum Albumin, BovineSpectrometry, Mass, Electrospray IonizationTrypsinJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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Jason S Page, Aleksey V Tolmachev, Keqi Tang, Richard D Smith. 2005. Variable low-mass filtering using an electrodynamic ion funnel.. https://doi.org/10.1002/jms.900

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