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Experiences in deer sperm cryopreservation under practical conditions--a pilot study.

Zoltán Zomborszky·Szabolcs Nagy·László Nánássy·Miklós Szabari·Szilárd Bodó
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Abstract

The genetic potential of the red and fallow deer populations in Hungary is well known. Conserving the variability in this excellent genetic material for game preservation is one of our most important task. The aim of the present pilot study was to test the logistical steps of a sperm processing and storing system in which deer sperm can be stored at a level that meets quality standards accepted for domestic animals. Moreover, two different semen extenders, commercially used for freezing bull semen, were compared from the viewpoint of applicability to freeze fallow deer sperm. Sperm was collected from epididymes of eight red stags (Cervus elaphus hippelaphus) and six fallow bucks (Dama dama) during the rutting season. Red deer samples were washed in Triladyl extender, while fallow deer samples were split and processed in Triladyl or Bioxcell extender. In the samples, which had a shorter time interval between the death of the animal and the sperm collection, the percentage of viable spermatozoa with intact acrosome was typically higher.

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AnimalsCell SurvivalCryopreservationDeerEpididymisHungaryMalePilot ProjectsSeasonsSemen PreservationSpermatozoaTissue and Organ HarvestingJournal ArticleResearch Support, Non-U.S. Gov't

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Zoltán Zomborszky, Szabolcs Nagy, László Nánássy, Miklós Szabari, Szilárd Bodó. 2005. Experiences in deer sperm cryopreservation under practical conditions--a pilot study.. https://doi.org/10.1016/j.anireprosci.2005.01.014

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