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Plane-wave fluorescence tomography with adaptive finite elements.

Amit Joshi·Wolfgang Bangerth·Kildong Hwang·John Rasmussen·Eva M Sevick-Muraca
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Abstract

We present three-dimensional fluorescence yield tomography of a tissue phantom in a noncontact reflectance imaging setup. The method employs planar illumination with modulated light and frequency domain fluorescence measurements made on the illumination plane. An adaptive finite-element algorithm is used to handle the ill-posed and computationally demanding inverse image reconstruction problem. Tomographic images of fluorescent targets buried at 1-2 cm depths from the illumination surface demonstrate the feasibility of fluorescence tomography from reflectance tomography in clinically relevant tissue volumes.

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AlgorithmsComputer SimulationFinite Element AnalysisImage EnhancementImage Interpretation, Computer-AssistedImaging, Three-DimensionalMicroscopy, FluorescenceModels, BiologicalPhantoms, ImagingReproducibility of ResultsSensitivity and SpecificityTomography, OpticalEvaluation StudyJournal Article

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Amit Joshi, Wolfgang Bangerth, Kildong Hwang, John Rasmussen, Eva M Sevick-Muraca. 2006-01-15. Plane-wave fluorescence tomography with adaptive finite elements.. https://doi.org/10.1364/ol.31.000193

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