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Mannose selection system used for cucumber transformation.

Zhengquan He·Zhenzhen Duan·Wei Liang·Faju Chen·Wei Yao·Hongwei Liang·Chaoyin Yue·Zongxiu Sun·Fan Chen·Jianwu Dai
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Abstract

The selectable marker system, which utilizes the pmi gene encoding for phosphomannose-isomerase that converts mannose-6-phosphate to fructose-6-phosphate, was adapted for Agrobacterium-mediated transformation of cucumber (Cucumis sativus L.). Only transformed cells were capable of utilizing mannose as a carbon source. The highest transformation frequency of 23% was obtained with 10 g/l mannose and 10 g/l sucrose in the medium. Molecular, genetic analysis, and PMI activity assay showed that the regenerated shoots contained the pmi gene and the gene was transmitted to the progeny in a Mendelian fashion. The results indicated that the mannose selection system, which is devoid of the disadvantages of antibiotic or herbicide selection, could be used for cucumber Agrobacterium-mediated transformation.

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Cucumis sativusGenetic VectorsGerminationMannosePlant TumorsPlants, Genetically ModifiedSeedsTransformation, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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Zhengquan He, Zhenzhen Duan, Wei Liang, Faju Chen, Wei Yao, Hongwei Liang, Chaoyin Yue, Zongxiu Sun, Fan Chen, Jianwu Dai. 2006-03-25. Mannose selection system used for cucumber transformation.. https://doi.org/10.1007/s00299-006-0156-z

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