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Microfluidic system for planar patch clamp electrode arrays.

Xiaohui Li·Kathryn G Klemic·Mark A Reed·Fred J Sigworth
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Abstract

We present a microfluidic system integrated with disposable cell interface partitions for simultaneous patch clamp recordings. Glass-supported poly(dimethylsiloxane) (PDMS) partitions, having a 2 microm air-blown aperture, were reversibly sealed to a microfluidic system including PDMS channels with isolation valves and microfabricated Ag/AgCl electrodes. Gigaseal recordings from RBL-1 cells were obtained with a 24% success rate. Simultaneous whole cell recordings from valve-isolated electrodes were obtained.

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AnimalsCell Culture TechniquesCell LineEquipment DesignEquipment Failure AnalysisFlow Injection AnalysisMast CellsMembrane PotentialsMicroelectrodesMicrofluidic Analytical TechniquesNanotechnologyPatch-Clamp TechniquesRatsEvaluation StudyJournal ArticleResearch Support, N.I.H., Extramural

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Xiaohui Li, Kathryn G Klemic, Mark A Reed, Fred J Sigworth. 2006. Microfluidic system for planar patch clamp electrode arrays.. https://doi.org/10.1021/nl060165r

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