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PubMed · 16674096

Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target.

Simon Ekström·Lars Wallman·Daniel Hök·György Marko-Varga·Thomas Laurell
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Abstract

A microfabricated proteomic sample preparation and sample presentation device, Integrated Selective Enrichment Target, (ISET), comprising an array of 96 perforated nanovials is described. Each perforated nanovial can be filled with solid-phase extraction media for purification and concentration of peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The validity of the ISET sample preparation is shown by analysis of low nM-pM standard samples, as well as biological samples. The ISET solid-phase extraction sample preparation was compared to ZipTip and MassPREP PROtarget sample preparation, demonstrating a superior performance with respect to number of detected peptides and signal intensity of detected peptides.

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CaseinsCrystallizationMicrofluidic Analytical TechniquesMiniaturizationNanotechnologyProteomicsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Simon Ekström, Lars Wallman, Daniel Hök, György Marko-Varga, Thomas Laurell. 2006. Miniaturized solid-phase extraction and sample preparation for MALDI MS using a microfabricated integrated selective enrichment target.. https://doi.org/10.1021/pr050434z

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