PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 1710700

[Morphometric analysis of immunocompetent cells].

A A Chumak·D A Bazyka·N V Beliaeva·A V Savran·V K Prakhova·Iu N Beliaev
View original source ↗Export metadata ↓

Abstract

The authors have developed the technique of morphometric analysis of human peripheral blood immunocompetent cells by means of Laborscal PSA-1, PSL-1 conductometric cytoanalyzer and DBK-010 computer-processing of the resultant histograms. The method was tried in examinations of more than 400 patients with various diseases and in studies of lymphocyte activation changes. Formulae for calculation of cellular diameters and volumes are offered, as is the program for histogram processing.

Explore related subjects

Cytological TechniquesElectronic Data ProcessingHumansIn Vitro TechniquesLymphocytesSoftwareJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

A A Chumak, D A Bazyka, N V Beliaeva, A V Savran, V K Prakhova, Iu N Beliaev. 1990. [Morphometric analysis of immunocompetent cells].. https://pubmed.ncbi.nlm.nih.gov/1710700/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2006

Optical tweezer micromanipulation of filamentous fungi.

Optical tweezers have been little used in experimental studies on filamentous fungi. We have built a simple, compact, easy-to-use, safe and robust optical tweezer system that can be used with brightfield, phase contrast, differential interference contrast and fluorescence optics on a standard research grade light microscope. We have used this optical tweezer system in a range of cell biology applications to trap and micromanipulate whole fungal cells, organelles within cells, and beads. We have demonstrated how optical tweezers can be used to: unambiguously determine whether hyphae are actively homing towards each other; move the Spitzenkörper and change the pattern of hyphal morphogenesis; make piconewton force measurements; mechanically stimulate hyphal tips; and deliver chemicals to localized regions of hyphae. Significant novel experimental findings from our study were that germ tubes generated significantly smaller growth forces than leading hyphae, and that both hyphal types exhibited growth responses to mechanical stimulation with optically trapped polystyrene beads. Germinated spores that had been optically trapped for 25min exhibited no deleterious effects with regard to conidial anastomosis tube growth, homing or fusion.

Cytological Techniques↗