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PubMed · 17132662

Alpha-particle radiobiological experiments using thin CR-39 detectors.

K F Chan·S Y M Siu·K E McClella·A K W Tse·B M F Lau·D Nikezic·B J Richardson·P K S Lam·W F Fong·K N Yu
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Abstract

The present paper studied the feasibility of applying comet assay to evaluate the DNA damage in individual HeLa cervix cancer cells after alpha-particle irradiation. We prepared thin CR-39 detectors (<20 microm) as cell-culture substrates, with UV irradiation to shorten the track formation time. After irradiation of the HeLa cells by alpha particles, the tracks on the underside of the CR-39 detector were developed by chemical etching in (while floating on) a 14 N KOH solution at 37 degrees C. Comet assay was then applied. Diffusion of DNA out of the cells could be generally observed from the images of stained DNA. The alpha-particle tracks corresponding to the comets developed on the underside of the CR-39 detectors could also be observed by just changing the focal plane of the confocal microscope.

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Alpha ParticlesCells, CulturedComet AssayDNADNA DamageEquipment DesignEquipment Failure AnalysisHeLa CellsHumansRadiobiologyRadiometryReproducibility of ResultsSensitivity and SpecificityTransducersEvaluation StudyJournal Article

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K F Chan, S Y M Siu, K E McClella, A K W Tse, B M F Lau, D Nikezic, B J Richardson, P K S Lam, W F Fong, K N Yu. 2006-11-28. Alpha-particle radiobiological experiments using thin CR-39 detectors.. https://doi.org/10.1093/rpd%2Fncl393

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