Polyacrylic microspheres as a solid phase for microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) of transferrin.

P Montagne; P Laroche; M L Cuilliere; D Riochet; O Flecheux; P Varcin; J Marchand; B Pau; J Duheille

doi:10.1080/01971529108055065

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Polyacrylic microspheres as a solid phase for microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) of transferrin.

P Montagne·P Laroche·M L Cuilliere·D Riochet·O Flecheux·P Varcin·J Marchand·B Pau·J Duheille

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Abstract

Polyfunctional hydrophilic microspheres (MS) can be produced by copolymerisation with gamma-irradiation of acrylic monomers. Transferrin (TRF) can be covalently bound to these MS by reaction between aldehyde groups of the MS and primary amino groups of the protein. MS-TRF conjugates thus obtained are agglutinated by specific antiserum and this agglutination is inhibited by free TRF. Agglutination and inhibition are quantified by measurement of the light scattered by MS-TRF conjugate clusters with a specially designed nephelometer, a process designated as microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) for TRF. Recovery, correlation and reproducibility studies, simultaneously performed in three different laboratories, show that this TRF immunoassay is accurate for a large concentration range. NEPHELIA (R) may appear as an alternative method for a large variety of molecules.

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Humans Immunoassay Microspheres Nephelometry and Turbidimetry Reference Standards Transferrin Journal Article Research Support, Non-U.S. Gov't

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P Montagne, P Laroche, M L Cuilliere, D Riochet, O Flecheux, P Varcin, J Marchand, B Pau, J Duheille. 1991. Polyacrylic microspheres as a solid phase for microparticle enhanced nephelometric immunoassay (NEPHELIA (R)) of transferrin.. https://doi.org/10.1080/01971529108055065

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