PubMed · 2491992
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J W Goldzieher, R L Young. 1989. Luteal phase deficiency.. https://doi.org/10.1016/s0015-0282(16)60460-0
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The haematopoietic stem cells remaining in the placenta after the umbilical cord has been cut can substitute for bone marrow in transplantations. It is argued that these cells should be retrieved and kept frozen as the child's personal biological spare part. A pilot, population-based collection of cord blood was carried out in a small provincial in Denmark. It was found that the pregnant women supported the concept (89%), that the midwives and technicians could carry out sampling and freezing after a short instruction period, and that the cost of sampling and storing for 75 years approx. DKK 12,000 per child. The project group is now seeking partners for further development of the concept.
Literature values for human plasma GSH vary over 10-fold despite the use of apparently valid analytical procedures for GSH measurement. The purpose of this study was to develop a procedure to minimize error in sample collection, processing and storage that could contribute to such differences. HPLC with fluorescence detection of dansyl derivatives was used for quantification. The results show that collection of blood with a butterfly needle and syringe reduces overestimation due to limited hemolysis and that use of a preservation solution designed to inhibit autooxidation and enzymatic degradation allows quantitative recovery of both GSH and GSSG. Stability tests showed that non-derivatized samples were stable for at least 2 months at - 80 degrees while dansyl derivatives were stable in the dark at 0-4 degrees for 12 months. Results from 59 healthy individuals (20-43 years) provided a mean (+/-1 SD) GSH value of 2.09+/-1.14 micromolar.
Sedimentation field-flow fractionation (SdFFF) offers great potential for the separation of submicrometer and micrometer-sized species. The availability of commercial instrumentation and the versatility of this method originated its success. At this stage of development, SdFFF techniques are mature enough for use in analytical research, development and even routine work. However, prior to their use, these techniques like any other methodologies, have to be validated. As the application of SdFFF techniques to cell separation is being constantly developed, we have investigated separation performance according to validation rules classically defined for separation methods (chromatography) in the case of cellular materials.