PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 2811187

[Selected immunologic parameters in uveitis].

E Wieczorek·P Hańczyc·E Prastowska
View original source ↗Export metadata ↓

Abstract

The behaviour of selected immunological parameters was checked in 38 patients with uveitis. The presence of immunological complexes was found in 23 patients and in 9 a positive Waaler-Rose's reaction. All patients showed a considerable fall in the percentage of the T lymphocytes with an increase of the B lymphocytes without markers as well as an increased concentration of the serum immunoglobulins of the M, A and G class.

Explore related subjects

Antigen-Antibody ComplexB-LymphocytesHumansImmunoglobulinsLeukocyte CountT-LymphocytesUveitisEnglish AbstractJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

E Wieczorek, P Hańczyc, E Prastowska. 1989. [Selected immunologic parameters in uveitis].. https://pubmed.ncbi.nlm.nih.gov/2811187/

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1992

Characterization of a lung-cancer-associated auto-antigen.

An antigen, protein X (Px), was purified from immune complexes isolated from malignant pleural effusions from patients with adenocarcinoma of the lung by EDTA treatment, PEG 8000 precipitation, protein A affinity chromatography, and Sephadex G-200 separation in the presence of 3 M NaCl. The purified antigen had a M(r) 17,000 by SDS-PAGE, and consisted of isoelectric species of pI 6.3 and 6.6. Purified Px recombined with Ig isolated from pleural fluids from patients with lung adenocarcinoma, but not with Ig from patients with breast carcinoma. Using an autologous human and heterologous chicken antibody, Px was found, by immunohistology, in the cytoplasm of some of the well-differentiated lung adenocarcinoma cells, but was not seen in normal lung or a variety of other malignant tissues. A liquid-phase competitive-inhibition RIA was developed. Over 30 ng/ml of Px were found in 9 of 15 pleural fluids from patients with lung carcinoma, none of 20 from patients with breast, ovary, stomach or colon cancer, and in 3 of 15 patients with unknown primary tumor. Our data suggest that Px may be a lung-cancer-associated autoantigen which can elicit a host humoral response in vivo.

Antigen-Antibody Complex↗
PubMed1992

Kinetic chromatographic sequential addition immunoassays using protein A affinity chromatography.

A new type of chromatographic immunoassay based on sequential addition is described. On a protein A column, the antibody, the sample containing the antigen, and then a known amount of antigen are sequentially injected. This assay is designed to shorten analysis times and reduce complexity of dual-column chromatographic immunoassays, circumvent desorption buffer interferences common to affinity chromatography, and eliminate the need for tagged molecules. This new technique is named kinetic immunochromatography sequential addition (KICQA). Because of its kinetic nature, flow rate will have a large effect on KICQA, and the impact of changing flow rate is studied extensively. By use of various amounts of antibody, the dynamic range of KICQA is shown to be selectable over 2.5 orders of magnitude. Finally, KICQA was used to determine transferrin and albumin in human serum. Both analytes show good agreement with their respective reference methods, and an albumin assay was performed in under 1 min.

Antigen-Antibody Complex↗