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PubMed · 2830054

Improved receptor assay for measuring digoxin activity.

B Bednarczyk·S J Soldin·I Gasinska·M D'Costa·L Perrot
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Abstract

We describe a receptor assay of digoxin activity involving Na+/K+-ATPase (EC 3.6.1.37) derived from human heart tissue. The procedure requires 500 microL of serum or plasma and incorporates one purification step, with Sep-Pak C18 cartridges (Waters). The method appears to be considerably more specific for digoxin and its cardio-active metabolites than are conventional immunoassays. Cardio-inactive metabolites and several digoxin-like factors cross react only slightly. The between-day coefficient of variation for the procedure ranged from 8.10% to 1.37% for digoxin concentrations between 0.5 and 5.0 nmol/L.

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AnimalsBlood ProteinsDigoxinDogsFatty AcidsFluorescence PolarizationHumansImmunoassayKidneyMyocardiumRadioligand AssaySodium-Potassium-Exchanging ATPaseSteroidsSwineComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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B Bednarczyk, S J Soldin, I Gasinska, M D'Costa, L Perrot. 1988. Improved receptor assay for measuring digoxin activity.. https://pubmed.ncbi.nlm.nih.gov/2830054/

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