PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 2999741

External eye diseases.

E L Stock
View original source ↗Export metadata ↓

Abstract

Diseases of the external eye can have several causes and many have similar symptoms, but a precise diagnosis of the specific disorder is crucial for proper treatment. Evaluation should begin with a complete medical history to ascertain any systemic disease that may affect the eyes. Physical examination should include determination of visual acuity, inspection of the tarsal and bulbar conjunctiva, and microscopic evaluation of the cornea. When external inflammatory disease is present, laboratory testing is required. Smears and cultures are the only reliable methods to determine the specific organism causing the infection. Treatment is then aimed at eradicating the underlying microbes.

Explore related subjects

Adenovirus Infections, HumanAnti-Bacterial AgentsChlamydia InfectionsChlamydia trachomatisConjunctivitis, BacterialConjunctivitis, ViralCorneal UlcerCromolyn SodiumEye DiseasesGlaucomaHerpesviridae InfectionsHumansInfant, NewbornKeratitisKeratoconjunctivitisOphthalmia NeonatorumPrimary Health CareUveitisJournal Article

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

E L Stock. 1985. External eye diseases.. https://doi.org/10.1080/00325481.1985.11699223

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed1996

Transduction of primitive human hematopoietic cells with recombinant adenovirus vectors.

We have examined the ability of recombinant adenoviral vectors to transduce human hematopoietic cells. Our findings indicate that adenovirus readily infects a large proportion of CD34+ cells. Using adenovirus vectors that transduce either a lacZ or an alkaline phosphatase reporter gene, we observed up to 45% of total CD34+ cells infected. Upon more detailed analysis, we observed comparable levels of transduction for CD34+/CD38- cells and for CD34+ cells in G(zero) phase of the cell cycle. Importantly, exposure to adenovirus resulted in negligible levels of toxicity as assayed by propidium iodide staining and colony-forming ability. Using adenovirus vectors, we also describe a model system for regulated gene expression in early hematopoietic tissues. CD34+ cells were simultaneously infected with two viruses, one carrying a TetR/VP16 transactivator (tTA) and the second carrying a tTA-dependent lacZ reporter gene. Using this approach, beta-gal expression was only observed upon coinfection with the transactivator vector. In addition, as shown previously (Gossen and Bujard, Proc Natl Acad Sci USA 89:5547, 1992), tetracycline was able to inhibit tTA mediated induction, thereby providing an effective means to regulate expression of the reporter gene. We conclude that recombinant adenovirus is an effective vehicle for transiently expressing genes in primitive human hematopoietic cells.

Adenovirus Infections, Human↗
PubMed1996

Multiplex polymerase chain reaction for adenovirus and herpes simplex virus in eye swabs.

Adenoviruses and herpes simplex virus (HSV) can cause clinically indistinguishable episodes of acute eye disease. Adenovirus infection is associated with nosocomial outbreaks and HSV may result in episodes of recurrent ocular inflammation. In a comparison of multiplex PCR for the two viral DNAs and virus isolation in cell culture, identical results were obtained for 18 of 20 specimens (positive for adenovirus in 5, HSV in 5, and negative in 8). One specimen was falsely negative for each viral DNA. Inclusion of human beta-globin primers in the adenovirus-HSV reaction was precluded by a consequential 10--100-fold reduction in sensitivity for the two viral targets and by the failure of beta-globin DNA amplification at the annealing temperature (45 degrees C) required to ensure detection of adenoviruses of serotypes 7 and 11 with the selected adenovirus primers. A single-target beta-globin PCR gave positive results with 19 of the 20 specimens prepared by treatment with proteinase K lysis buffer, indicating the effectiveness of this simple DNA extraction procedure. Nonetheless, the availability of effective antiviral therapy for HSV made monitoring for extraction failure using human primers crucial to avoid false-negative results for HSV DNA. Adenovirus-HSV PCR has considerable potential for the rapid diagnosis of viral eye disease particularly if beta-globin primers can be included in the reaction.

Adenovirus Infections, Human↗