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Receptor radioligand system for measuring digoxin activity.

E L Manchester·E Giesbrecht·S J Soldin
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Abstract

The purpose of this study was to develop and evaluate a receptor radioligand assay (RRA) for the measurement of digoxin, its metabolites, and endoxin and to compare the results of this assay with those of a fluorescence polarization immunoassay (FPIA) for digoxin. The within-run coefficients of variations for the RRA at digoxin concentrations of 2.0 and 5.0 nmol/L were 21 and 10%, respectively. Serum samples collected from 33 volunteers (not on digoxin medication) measured in the RRA resulted in levels less than 1.23 nmol/L digoxin equivalents (1.23 nmol/L is the lower level of sensitivity for this system). Serum samples collected from 29 patients receiving digoxin for therapeutic purposes and measured in the RRA gave a mean of 3.39 nmol/L digoxin equivalents. The samples collected from the patients receiving digoxin were also measured using FPIA with a mean of 1.75 nmol/L digoxin. The interference of progesterone, 11-alpha-hydroxyprogesterone, cortisone, and six digoxin metabolites on the binding of 3H-ouabain in the RRA was also evaluated.

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AnimalsBinding, CompetitiveBiotransformationDigoxinDogsHumansIn Vitro TechniquesKidneyOuabainRadioligand AssayReceptors, DrugSodium-Potassium-Exchanging ATPaseSteroidsJournal ArticleResearch Support, Non-U.S. Gov't

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E L Manchester, E Giesbrecht, S J Soldin. 1987. Receptor radioligand system for measuring digoxin activity.. https://doi.org/10.1097/00007691-198703000-00011

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